DOI: 10.1091/mbc.9.4.931. Homology-dependent Gene Silencing in<i>Paramecium</i>

Homology-dependent Gene Silencing inParamecium

10.1091/mbc.9.4.931

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for allParamecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation of specific mutant phenotypes thus providing a potentially powerful tool to study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant.

Bibliography

Ruiz, F., VayssiÃ©, L., Klotz, C., Sperling, L., & Madeddu, L. (1998). Homology-dependent Gene Silencing inParamecium. Molecular Biology of the Cell, 9(4), 931â943.

Authors 5

Françoise Ruiz (first)
Laurence Vayssié (additional)
Catherine Klotz (additional)
Linda Sperling (additional)
Luisa Madeddu (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:36 p.m.)
Indexed	1 day, 13 hours ago (Sept. 3, 2025, 6:33 a.m.)
Issued	27 years, 5 months ago (April 1, 1998)
Published	27 years, 5 months ago (April 1, 1998)
Published Print	27 years, 5 months ago (April 1, 1998)

Funders 0

None

BibTeX

@article{Ruiz_1998, title={Homology-dependent Gene Silencing inParamecium}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.4.931}, DOI={10.1091/mbc.9.4.931}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Ruiz, Françoise and Vayssié, Laurence and Klotz, Catherine and Sperling, Linda and Madeddu, Luisa}, year={1998}, month=apr, pages={931–943} }

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  "abstract": "<jats:p>Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for allParamecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation of specific mutant phenotypes thus providing a potentially powerful tool to study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant.</jats:p>",
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