DOI: 10.1091/mbc.9.3.585. SNAP-25 Palmitoylation and Plasma Membrane Targeting Require a Functional Secretory Pathway

SNAP-25 Palmitoylation and Plasma Membrane Targeting Require a Functional Secretory Pathway

10.1091/mbc.9.3.585

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Synaptosomal-associated protein of 25 kDa (SNAP-25) is a palmitoylated membrane protein essential for neurotransmitter release from synaptic terminals. We used neuronal cell lines to study the biosynthesis and posttranslational processing of SNAP-25 to investigate how palmitoylation contributes to the subcellular localization of the protein. SNAP-25 was synthesized as a soluble protein that underwent palmitoylation approximately 20 min after synthesis. Palmitoylation of the protein coincided with its stable membrane association. Treatment of cells with brefeldin A or other disrupters of transport inhibited palmitoylation of newly synthesized SNAP-25 and abolished membrane association. These results demonstrate that the processing of SNAP-25 and its targeting to the plasma membrane depend on an intact transport mechanism along the exocytic pathway. The kinetics of SNAP-25 palmitoylation and membrane association and the sensitivity of these parameters to brefeldin A suggest a novel trafficking pathway for targeting proteins to the plasma membrane. In vitro, SNAP-25 stably associated with membranes was not released from the membrane after chemical deacylation. We propose that palmitoylation of SNAP-25 is required for initial membrane targeting of the protein but that other interactions can maintain membrane association in the absence of fatty acylation.

Bibliography

Gonzalo, S., & Linder, M. E. (1998). SNAP-25 Palmitoylation and Plasma Membrane Targeting Require a Functional Secretory Pathway. Molecular Biology of the Cell, 9(3), 585â597.

Authors 2

Susana Gonzalo (first)
Maurine E. Linder (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:35 p.m.)
Indexed	3 weeks, 6 days ago (July 28, 2025, 2:27 a.m.)
Issued	27 years, 5 months ago (March 1, 1998)
Published	27 years, 5 months ago (March 1, 1998)
Published Print	27 years, 5 months ago (March 1, 1998)

Funders 0

None

BibTeX

@article{Gonzalo_1998, title={SNAP-25 Palmitoylation and Plasma Membrane Targeting Require a Functional Secretory Pathway}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.3.585}, DOI={10.1091/mbc.9.3.585}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Gonzalo, Susana and Linder, Maurine E.}, year={1998}, month=mar, pages={585–597} }

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