DOI: 10.1091/mbc.9.10.2839. cut11+: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe

cut11+: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe

10.1091/mbc.9.10.2839

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The “cut” mutants of Schizosaccharomyces pombeare defective in spindle formation and/or chromosome segregation, but they proceed through the cell cycle, resulting in lethality. Analysis of temperature-sensitive alleles of cut11+suggests that this gene is required for the formation of a functional bipolar spindle. Defective spindle structure was revealed with fluorescent probes for tubulin and DNA. Three-dimensional reconstruction of mutant spindles by serial sectioning and electron microscopy showed that the spindle pole bodies (SPBs) either failed to complete normal duplication or were free floating in the nucleoplasm. Localization of Cut11p tagged with the green fluorescent protein showed punctate nuclear envelope staining throughout the cell cycle and SPBs staining from early prophase to mid anaphase. This SPB localization correlates with the time in the cell cycle when SPBs are inserted into the nuclear envelope. Immunoelectron microscopy confirmed the localization of Cut11p to mitotic SPBs and nuclear pore complexes. Cloning and sequencing showed thatcut11+encodes a novel protein with seven putative membrane-spanning domains and homology to theSaccharomyces cerevisiae gene NDC1. These data suggest that Cut11p associates with nuclear pore complexes and mitotic SPBs as an anchor in the nuclear envelope; this role is essential for mitosis.

Bibliography

West, R. R., Vaisberg, E. V., Ding, R., Nurse, P., & McIntosh, J. R. (1998). cut11+: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe. Molecular Biology of the Cell, 9(10), 2839â2855.

Authors 5

Robert R. West (first)
Elena V. Vaisberg (additional)
Rubai Ding (additional)
Paul Nurse (additional)
J. Richard McIntosh (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:12 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:34 p.m.)
Indexed	2 months, 1 week ago (June 7, 2025, 6:43 a.m.)
Issued	26 years, 10 months ago (Oct. 1, 1998)
Published	26 years, 10 months ago (Oct. 1, 1998)
Published Print	26 years, 10 months ago (Oct. 1, 1998)

Funders 0

None

BibTeX

@article{West_1998, title={cut11+: A Gene Required for Cell Cycle-dependent Spindle Pole Body Anchoring in the Nuclear Envelope and Bipolar Spindle Formation inSchizosaccharomyces pombe}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.10.2839}, DOI={10.1091/mbc.9.10.2839}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={West, Robert R. and Vaisberg, Elena V. and Ding, Rubai and Nurse, Paul and McIntosh, J. Richard}, editor={Yanagida, Mitsuhiro}, year={1998}, month=oct, pages={2839–2855} }

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