DOI: 10.1091/mbc.9.1.173. Morphology of the Yeast Endocytic Pathway

Morphology of the Yeast Endocytic Pathway

10.1091/mbc.9.1.173

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Positively charged Nanogold (Nanoprobes, Stony Brook, NY) has been developed as a new marker to follow the endocytic pathway in yeast. Positively charged Nanogold binds extensively to the surface of yeast spheroplasts and is internalized in an energy-dependent manner. Internalization of gold is blocked in the end3 mutant. During a time course of incubation of yeast spheroplasts with positively charged Nanogold at 15°C, the gold was detected sequentially in small vesicles, a peripheral, vesicular/tubular compartment that we designate as an early endosome, a multivesicular body corresponding to the late endosome near the vacuole, and in the vacuole. Experiments examining endocytosis in the sec18mutant showed an accumulation of positively charged Nanogold in approximately 30–50 nm diameter vesicles. These vesicles most likely represent the primary endocytic vesicles as no other intermediates were detected in the mutant cells, and they correspond in size to the first vesicles detected in wild-type spheroplasts at 15°C. These data lend strong support to the idea that the internalization step of endocytosis in yeast involves formation of small vesicles of uniform size from the plasma membrane.

Bibliography

Prescianotto-Baschong, C., & Riezman, H. (1998). Morphology of the Yeast Endocytic Pathway. Molecular Biology of the Cell, 9(1), 173â189.

Authors 2

Cristina Prescianotto-Baschong (first)
Howard Riezman (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:12 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:34 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 8, 2024, 7:46 p.m.)
Issued	27 years, 8 months ago (Jan. 1, 1998)
Published	27 years, 8 months ago (Jan. 1, 1998)
Published Print	27 years, 8 months ago (Jan. 1, 1998)

Funders 0

None

BibTeX

@article{Prescianotto_Baschong_1998, title={Morphology of the Yeast Endocytic Pathway}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.1.173}, DOI={10.1091/mbc.9.1.173}, number={1}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Prescianotto-Baschong, Cristina and Riezman, Howard}, year={1998}, month=jan, pages={173–189} }

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