DOI: 10.1091/mbc.8.12.2487. Yeast Cycloheximide-resistant<i>crl</i>Mutants Are Proteasome Mutants Defective in Protein Degradation

Yeast Cycloheximide-resistantcrlMutants Are Proteasome Mutants Defective in Protein Degradation

10.1091/mbc.8.12.2487

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

In 1988 McCusker and Haber generated a series of mutants which are resistant to the minimum inhibitory concentration of the protein synthesis inhibitor cycloheximide. These cycloheximide-resistant, temperature-sensitive (crl) mutants, in addition, exhibited other pleiotropic phenotypes, e.g., incorrect response to starvation, hypersensitivity against amino acid analogues, and other protein synthesis inhibitors. Temperature sensitivity of one of these mutants, crl3–2, had been found to be suppressed by a mutation, SCL1–1, which resided in an α-type subunit of the 20S proteasome. We cloned the CRL3 gene by complementation and found CRL3 to be identical to theSUG1/CIM3 gene coding for a subunit of the 19S cap complex of the 26S proteasome. Another mutation, crl21, revealed to be allelic with the 20S proteasomal gene PRE3. crl3–2 and crl21 mutant cells show significant defects in proteasome-dependent proteolysis, whereas theSCL1–1 suppressor mutation causes partial restoration of crl3–2-induced proteolytic defects. Notably, cycloheximide resistance was also detected for other proteolytically deficient proteasome mutants (pre1–1,pre2–1, pre3–1, pre4–1). Moreover, proteasomal genes were found within genomic sequences of 9 of 13 chromosomal loci to which crl mutations had been mapped. We therefore assume that most if not all crlmutations reside in the proteasome and that phenotypes found are a result of defective protein degradation.

Bibliography

Gerlinger, U.-M., GÃ¼ckel, R., Hoffmann, M., Wolf, D. H., & Hilt, W. (1997). Yeast Cycloheximide-resistantcrlMutants Are Proteasome Mutants Defective in Protein Degradation. Molecular Biology of the Cell, 8(12), 2487â2499.

Authors 5

Uwe-M. Gerlinger (first)
Roland Gückel (additional)
Michael Hoffmann (additional)
Dieter H. Wolf (additional)
Wolfgang Hilt (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:12 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:34 p.m.)
Indexed	2 months, 2 weeks ago (June 3, 2025, 12:57 a.m.)
Issued	27 years, 8 months ago (Dec. 1, 1997)
Published	27 years, 8 months ago (Dec. 1, 1997)
Published Print	27 years, 8 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Gerlinger_1997, title={Yeast Cycloheximide-resistantcrlMutants Are Proteasome Mutants Defective in Protein Degradation}, volume={8}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.8.12.2487}, DOI={10.1091/mbc.8.12.2487}, number={12}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Gerlinger, Uwe-M. and Gückel, Roland and Hoffmann, Michael and Wolf, Dieter H. and Hilt, Wolfgang}, year={1997}, month=dec, pages={2487–2499} }

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  "abstract": "<jats:p>In 1988 McCusker and Haber generated a series of mutants which are resistant to the minimum inhibitory concentration of the protein synthesis inhibitor cycloheximide. These cycloheximide-resistant, temperature-sensitive (crl) mutants, in addition, exhibited other pleiotropic phenotypes, e.g., incorrect response to starvation, hypersensitivity against amino acid analogues, and other protein synthesis inhibitors. Temperature sensitivity of one of these mutants, crl3\u20132, had been found to be suppressed by a mutation, SCL1\u20131, which resided in an \u03b1-type subunit of the 20S proteasome. We cloned the CRL3 gene by complementation and found CRL3 to be identical to theSUG1/CIM3 gene coding for a subunit of the 19S cap complex of the 26S proteasome. Another mutation, crl21, revealed to be allelic with the 20S proteasomal gene PRE3. crl3\u20132 and crl21 mutant cells show significant defects in proteasome-dependent proteolysis, whereas theSCL1\u20131 suppressor mutation causes partial restoration of crl3\u20132-induced proteolytic defects. Notably, cycloheximide resistance was also detected for other proteolytically deficient proteasome mutants (pre1\u20131,pre2\u20131, pre3\u20131, pre4\u20131). Moreover, proteasomal genes were found within genomic sequences of 9 of 13 chromosomal loci to which crl mutations had been mapped. We therefore assume that most if not all crlmutations reside in the proteasome and that phenotypes found are a result of defective protein degradation.</jats:p>",
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