DOI: 10.1091/mbc.8.11.2145. Coordinate Regulation of G- and C Strand Length during New Telomere Synthesis

Coordinate Regulation of G- and C Strand Length during New Telomere Synthesis

10.1091/mbc.8.11.2145

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

We have used the ciliate Euplotes to study the role of DNA polymerase in telomeric C strand synthesis.Euplotes provides a unique opportunity to study C strand synthesis without the complication of simultaneous DNA replication because millions of new telomeres are made at a stage in the life cycle when no general DNA replication takes place. Previously we showed that the C-strands of newly synthesized telomeres have a precisely controlled length while the G-strands are more heterogeneous. This finding suggested that, although synthesis of the G-strand (by telomerase) is the first step in telomere addition, a major regulatory step occurs during subsequent C strand synthesis. We have now examined whether G- and C strand synthesis might be regulated coordinately rather than by two independent mechanisms. We accomplished this by determining what happens to G- and C strand length if C strand synthesis is partially inhibited by aphidicolin. Aphidicolin treatment caused a general lengthening of the G-strands and a large increase in C strand heterogeneity. This concomitant change in both the G- and C strand length indicates that synthesis of the two strands is coordinated. Since aphidicolin is a very specific inhibitor of DNA polα and polδ, our results suggest that this coordinate length regulation is mediated by DNA polymerase.

Bibliography

Fan, X., & Price, C. M. (1997). Coordinate Regulation of G- and C Strand Length during New Telomere Synthesis. Molecular Biology of the Cell, 8(11), 2145â2155.

Authors 2

Xinqing Fan (first)
Carolyn Mary Price (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:12 p.m.)
Deposited	5 years ago (July 29, 2020, 11:21 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 5, 2025, 8:33 a.m.)
Issued	27 years, 9 months ago (Nov. 1, 1997)
Published	27 years, 9 months ago (Nov. 1, 1997)
Published Print	27 years, 9 months ago (Nov. 1, 1997)

Funders 0

None

BibTeX

@article{Fan_1997, title={Coordinate Regulation of G- and C Strand Length during New Telomere Synthesis}, volume={8}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.8.11.2145}, DOI={10.1091/mbc.8.11.2145}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Fan, Xinqing and Price, Carolyn Mary}, year={1997}, month=nov, pages={2145–2155} }

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  "abstract": "<jats:p>We have used the ciliate Euplotes to study the role of DNA polymerase in telomeric C strand synthesis.Euplotes provides a unique opportunity to study C strand synthesis without the complication of simultaneous DNA replication because millions of new telomeres are made at a stage in the life cycle when no general DNA replication takes place. Previously we showed that the C-strands of newly synthesized telomeres have a precisely controlled length while the G-strands are more heterogeneous. This finding suggested that, although synthesis of the G-strand (by telomerase) is the first step in telomere addition, a major regulatory step occurs during subsequent C strand synthesis. We have now examined whether G- and C strand synthesis might be regulated coordinately rather than by two independent mechanisms. We accomplished this by determining what happens to G- and C strand length if C strand synthesis is partially inhibited by aphidicolin. Aphidicolin treatment caused a general lengthening of the G-strands and a large increase in C strand heterogeneity. This concomitant change in both the G- and C strand length indicates that synthesis of the two strands is coordinated. Since aphidicolin is a very specific inhibitor of DNA pol\u03b1 and pol\u03b4, our results suggest that this coordinate length regulation is mediated by DNA polymerase.</jats:p>",
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