DOI: 10.1091/mbc.8.11.2119. Nuclear Pore Complex Number and Distribution throughout the<i>Saccharomyces cerevisiae</i>Cell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes

Nuclear Pore Complex Number and Distribution throughout theSaccharomyces cerevisiaeCell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes

10.1091/mbc.8.11.2119

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of serially sectioned cells. Nuclei of 32 haploid cells at various points in the cell cycle were modeled and found to contain between 65 and 182 NPCs. Morphological markers, such as cell shape and nuclear shape, were used to determine the cell cycle stage of the cell being examined. NPC number was correlated with cell cycle stage to reveal that the number of NPCs increases steadily, beginning in G1-phase, suggesting that NPC assembly occurs continuously throughout the cell cycle. However, the accumulation of nuclear envelope observed during the cell cycle, indicated by nuclear surface area, is not continuous at the same rate, such that the density of NPCs per unit area of nuclear envelope peaks in apparent S-phase cells. Analysis of the nuclear envelope reconstructions also revealed no preferred NPC-to-NPC distance. However, NPCs were found in large clusters over regions of the nuclear envelope. Interestingly, clusters of NPCs were most pronounced in early mitotic nuclei and were found to be associated with the spindle pole bodies, but the functional significance of this association is unknown.

Bibliography

Winey, M., Yarar, D., Giddings, T. H., & Mastronarde, D. N. (1997). Nuclear Pore Complex Number and Distribution throughout theSaccharomyces cerevisiaeCell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes. Molecular Biology of the Cell, 8(11), 2119â2132.

Authors 4

Mark Winey (first)
Defne Yarar (additional)
Thomas H. Giddings (additional)
David N. Mastronarde (additional)

References 20 Referenced 201

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:12 p.m.)
Deposited	5 years ago (July 29, 2020, 11:21 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 3, 2025, 12:16 a.m.)
Issued	27 years, 9 months ago (Nov. 1, 1997)
Published	27 years, 9 months ago (Nov. 1, 1997)
Published Print	27 years, 9 months ago (Nov. 1, 1997)

Funders 0

None

BibTeX

@article{Winey_1997, title={Nuclear Pore Complex Number and Distribution throughout theSaccharomyces cerevisiaeCell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes}, volume={8}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.8.11.2119}, DOI={10.1091/mbc.8.11.2119}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Winey, Mark and Yarar, Defne and Giddings, Thomas H. and Mastronarde, David N.}, year={1997}, month=nov, pages={2119–2132} }

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