DOI: 10.1091/mbc.2.9.699. Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells.

Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells.

10.1091/mbc.2.9.699

Crossref journal-article

American Society for Cell Biology (ASCB)

Cell Regulation (1076)

Abstract

The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high molecular weight (HMW) forms of bFGF, the 18-kDa form, or all forms were examined. Cells producing the 18 kDa or all forms of bFGF were transformed at high levels of growth factor expression but were nontransformed at low levels. Cell producing low levels of HMW forms of bFGF were growth impaired when compared with the parental cells. These cells tended to form multinucleated giant cells, did not grow in soft agar, were nontumorigenic, had a normal bFGF receptor number, and had a nontransformed morphology. Cells expressing high levels of HMW bFGFs had a transformed morphology and were tumorigenic. These data suggest a specific functional role for HMWbFGF.

Bibliography

Quarto, N., Talarico, D., Florkiewicz, R., & Rifkin, D. B. (1991). Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells. Cell Regulation, 2(9), 699â708.

Authors 4

N Quarto (first)
D Talarico (additional)
R Florkiewicz (additional)
D B Rifkin (additional)

References 0 Referenced 81

None

Dates

Type	When
Created	12 years ago (Aug. 16, 2013, 6:47 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 11:10 a.m.)
Indexed	4 hours, 14 minutes ago (Sept. 4, 2025, 9:50 a.m.)
Issued	34 years ago (Sept. 1, 1991)
Published	34 years ago (Sept. 1, 1991)
Published Print	34 years ago (Sept. 1, 1991)

Funders 0

None

BibTeX

@article{Quarto_1991, title={Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells.}, volume={2}, ISSN={1044-2030}, url={http://dx.doi.org/10.1091/mbc.2.9.699}, DOI={10.1091/mbc.2.9.699}, number={9}, journal={Cell Regulation}, publisher={American Society for Cell Biology (ASCB)}, author={Quarto, N and Talarico, D and Florkiewicz, R and Rifkin, D B}, year={1991}, month=sep, pages={699–708} }

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