DOI: 10.1091/mbc.2.10.851. An ATP-binding membrane protein is required for protein translocation across the endoplasmic reticulum membrane.

An ATP-binding membrane protein is required for protein translocation across the endoplasmic reticulum membrane.

10.1091/mbc.2.10.851

Crossref journal-article

American Society for Cell Biology (ASCB)

Cell Regulation (1076)

Abstract

The role of nucleotides in providing energy for polypeptide transfer across the endoplasmic reticulum (ER) membrane is still unknown. To address this question, we treated ER-derived mammalian microsomal vesicles with a photoactivatable analogue of ATP, 8-N3ATP. This treatment resulted in a progressive inhibition of translocation activity. Approximately 20 microsomal membrane proteins were labeled by [alpha 32P]8-N3ATP. Two of these were identified as proteins with putative roles in translocation, alpha signal sequence receptor (SSR), the 35-kDa subunit of the signal sequence receptor complex, and ER-p180, a putative ribosome receptor. We found that there was a positive correlation between inactivation of translocation activity and photolabeling of alpha SSR. In contrast, our data demonstrate that the ATP-binding domain of ER-p180 is dispensable for translocation activity and does not contribute to the observed 8-N3ATP sensitivity of the microsomal vesicles.

Bibliography

Zimmerman, D. L., & Walter, P. (1991). An ATP-binding membrane protein is required for protein translocation across the endoplasmic reticulum membrane. Cell Regulation, 2(10), 851â859.

Authors 2

D L Zimmerman (first)
P Walter (additional)

References 0 Referenced 13

None

Dates

Type	When
Created	12 years ago (Aug. 16, 2013, 6:42 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 11:09 a.m.)
Indexed	1 year, 7 months ago (Jan. 10, 2024, 5 p.m.)
Issued	33 years, 10 months ago (Oct. 1, 1991)
Published	33 years, 10 months ago (Oct. 1, 1991)
Published Print	33 years, 10 months ago (Oct. 1, 1991)

Funders 0

None

BibTeX

@article{Zimmerman_1991, title={An ATP-binding membrane protein is required for protein translocation across the endoplasmic reticulum membrane.}, volume={2}, ISSN={1044-2030}, url={http://dx.doi.org/10.1091/mbc.2.10.851}, DOI={10.1091/mbc.2.10.851}, number={10}, journal={Cell Regulation}, publisher={American Society for Cell Biology (ASCB)}, author={Zimmerman, D L and Walter, P}, year={1991}, month=oct, pages={851–859} }

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