DOI: 10.1091/mbc.12.9.2567. <i>ASH1</i>mRNA Localization in Three Acts

10.1091/mbc.12.9.2567

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Novel green fluorescent protein (GFP) labeling techniques targeting specific mRNA transcripts reveal discrete phases of mRNA localization in yeast: packaging, transport, and docking. In budding yeast,ASH1 mRNA is translocated via actin and myosin to the tip of growing cells. A GFP-decorated reporter transcript containing the ASH1 3′ untranslated region gRNAASH1forms spots of fluorescence localized to a cortical domain at the bud tip, relocates to the mother-bud neck before cell separation, and finally migrates to the incipient bud site before the next budding cycle. The correct positioning of the mRNA requires at least six proteins: She1p-5p and Bud6p/Aip3p. gRNAASH1localization in mutant strains identified three functional categories for the She proteins: mRNA particle formation (She2p and She4p), mRNA transport into the bud (She1p/Myo4p and She3p), and mRNA tethering at the bud tip (She5p/Bni1p and Bud6p/Aip3p). Because localization of the mRNA within the bud does not a priori restrict the translated protein, we examine the distribution of a mother-specific protein (Yta6p) translated from a mRNA directed into the bud. Yta6p remains associated with the mother cortex despite localization of the mRNA to the bud. This video essay traces the life history of a localized mRNA transcript, describes the roles of proteins required to polarize and anchor the mRNA, and demonstrates at least one instance where mRNA localization does not effect protein localization.

Bibliography

Beach, D. L., & Bloom, K. (2001). ASH1mRNA Localization in Three Acts. Molecular Biology of the Cell, 12(9), 2567â2577.

Authors 2

Dale L. Beach (first)
Kerry Bloom (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 8:20 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:46 p.m.)
Indexed	1 year, 1 month ago (July 3, 2024, 11:53 a.m.)
Issued	24 years ago (Sept. 1, 2001)
Published	24 years ago (Sept. 1, 2001)
Published Print	24 years ago (Sept. 1, 2001)

Funders 0

None

BibTeX

@article{Beach_2001, title={ASH1mRNA Localization in Three Acts}, volume={12}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.12.9.2567}, DOI={10.1091/mbc.12.9.2567}, number={9}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Beach, Dale L. and Bloom, Kerry}, editor={Koshland, Douglas}, year={2001}, month=sep, pages={2567–2577} }

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