DOI: 10.1091/mbc.12.8.2290. Focal Adhesion Kinase and p130Cas Mediate Both Sarcomeric Organization and Activation of Genes Associated with Cardiac Myocyte Hypertrophy

Focal Adhesion Kinase and p130Cas Mediate Both Sarcomeric Organization and Activation of Genes Associated with Cardiac Myocyte Hypertrophy

10.1091/mbc.12.8.2290

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Hypertrophic terminally differentiated cardiac myocytes show increased sarcomeric organization and altered gene expression. Previously, we established a role for the nonreceptor tyrosine kinase Src in signaling cardiac myocyte hypertrophy. Here we report evidence that p130Cas (Cas) and focal adhesion kinase (FAK) regulate this process. In neonatal cardiac myocytes, tyrosine phosphorylation of Cas and FAK increased upon endothelin (ET) stimulation. FAK, Cas, and paxillin were localized in sarcomeric Z-lines, suggesting that the Z-line is an important signaling locus in these cells. Cas, alone or in cooperation with Src, modulated basal and ET-stimulated atrial natriuretic peptide (ANP) gene promoter activity, a marker of cardiac hypertrophy. Expression of the C-terminal focal adhesion-targeting domain of FAK interfered with localization of endogenous FAK to Z-lines. Expression of the Cas-binding proline-rich region 1 of FAK hindered association of Cas with FAK and impaired the structural stability of sarcomeres. Collectively, these results suggest that interaction of Cas with FAK, together with their localization to Z-lines, is critical to assembly of sarcomeric units in cardiac myocytes in culture. Moreover, expression of the focal adhesion-targeting and/or the Cas-binding proline-rich regions of FAK inhibited ANP promoter activity and suppressed ET-induced ANP and brain natriuretic peptide gene expression. In summary, assembly of signaling complexes that include the focal adhesion proteins Cas, FAK, and paxillin at Z-lines in the cardiac myocyte may regulate, either directly or indirectly, both cytoskeletal organization and gene expression associated with cardiac myocyte hypertrophy.

Bibliography

KovacÌicÌ-MilivojeviÄ, B., Roediger, F., Almeida, E. A. C., Damsky, C. H., Gardner, D. G., & IliÄ, D. (2001). Focal Adhesion Kinase and p130Cas Mediate Both Sarcomeric Organization and Activation of Genes Associated with Cardiac Myocyte Hypertrophy. Molecular Biology of the Cell, 12(8), 2290â2307.

Authors 6

Branka Kovac̆ic̆-Milivojević (first)
Frederick Roediger (additional)
Eduardo A.C. Almeida (additional)
Caroline H. Damsky (additional)
David G. Gardner (additional)
Duško Ilić (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 8:18 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:45 p.m.)
Indexed	1 year, 1 month ago (July 17, 2024, 6:32 a.m.)
Issued	24 years, 1 month ago (Aug. 1, 2001)
Published	24 years, 1 month ago (Aug. 1, 2001)
Published Print	24 years, 1 month ago (Aug. 1, 2001)

Funders 0

None

BibTeX

@article{Kovac_ic_Milivojevi__2001, title={Focal Adhesion Kinase and p130Cas Mediate Both Sarcomeric Organization and Activation of Genes Associated with Cardiac Myocyte Hypertrophy}, volume={12}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.12.8.2290}, DOI={10.1091/mbc.12.8.2290}, number={8}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Kovac̆ic̆-Milivojević, Branka and Roediger, Frederick and Almeida, Eduardo A.C. and Damsky, Caroline H. and Gardner, David G. and Ilić, Duško}, editor={Beckerle, Mary C.}, year={2001}, month=aug, pages={2290–2307} }

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