DOI: 10.1091/mbc.12.5.1293. Involvement of the Ubiquitin/Proteasome System in Sorting of the Interleukin 2 Receptor β Chain to Late Endocytic Compartments

Involvement of the Ubiquitin/Proteasome System in Sorting of the Interleukin 2 Receptor β Chain to Late Endocytic Compartments

10.1091/mbc.12.5.1293

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Down-regulation of cell surface growth factor receptors plays a key role in the tight control of cellular responses. Recent reports suggest that the ubiquitin system, in addition to participating in degradation by the proteasome of cytosolic and nuclear proteins, might also be involved in the down-regulation of various membrane receptors. We have previously characterized a signal in the cytosolic part of the interleukin 2 receptor β chain (IL2Rβ) responsible for its targeting to late endosomes/lysosomes. In this report, the role of the ubiquitin/proteasome system on the intracellular fate of IL2Rβ was investigated. Inactivation of the cellular ubiquitination machinery in ts20 cells, which express a thermolabile ubiquitin-activating enzyme E1, leads to a significant decrease in the degradation rate of IL2Rβ, with little effect on its internalization. In addition, we show that a fraction of IL2Rβ can be monoubiquitinated. Furthermore, mutation of the lysine residues of the cytosolic region of a chimeric receptor carrying the IL2Rβ targeting signal resulted in a decreased degradation rate. When cells expressing IL2Rβ were treated either by proteasome or lysosome inhibitors, a significant decrease in receptor degradation was observed. Our data show that ubiquitination is required for the sorting of IL2Rβ toward degradation. They also indicate that impairment of proteasome function might more generally affect intracellular routing.

Bibliography

Rocca, A., Lamaze, C., Subtil, A., & Dautry-Varsat, A. (2001). Involvement of the Ubiquitin/Proteasome System in Sorting of the Interleukin 2 Receptor Î² Chain to Late Endocytic Compartments. Molecular Biology of the Cell, 12(5), 1293â1301.

Authors 4

Anna Rocca (first)
Christophe Lamaze (additional)
Agathe Subtil (additional)
Alice Dautry-Varsat (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 8:13 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:45 p.m.)
Indexed	3 months ago (May 29, 2025, 3:45 p.m.)
Issued	24 years, 4 months ago (May 1, 2001)
Published	24 years, 4 months ago (May 1, 2001)
Published Print	24 years, 4 months ago (May 1, 2001)

Funders 0

None

BibTeX

@article{Rocca_2001, title={Involvement of the Ubiquitin/Proteasome System in Sorting of the Interleukin 2 Receptor β Chain to Late Endocytic Compartments}, volume={12}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.12.5.1293}, DOI={10.1091/mbc.12.5.1293}, number={5}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Rocca, Anna and Lamaze, Christophe and Subtil, Agathe and Dautry-Varsat, Alice}, editor={Riezman, Howard}, year={2001}, month=may, pages={1293–1301} }

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  "abstract": "<jats:p>Down-regulation of cell surface growth factor receptors plays a key role in the tight control of cellular responses. Recent reports suggest that the ubiquitin system, in addition to participating in degradation by the proteasome of cytosolic and nuclear proteins, might also be involved in the down-regulation of various membrane receptors. We have previously characterized a signal in the cytosolic part of the interleukin 2 receptor \u03b2 chain (IL2R\u03b2) responsible for its targeting to late endosomes/lysosomes. In this report, the role of the ubiquitin/proteasome system on the intracellular fate of IL2R\u03b2 was investigated. Inactivation of the cellular ubiquitination machinery in ts20 cells, which express a thermolabile ubiquitin-activating enzyme E1, leads to a significant decrease in the degradation rate of IL2R\u03b2, with little effect on its internalization. In addition, we show that a fraction of IL2R\u03b2 can be monoubiquitinated. Furthermore, mutation of the lysine residues of the cytosolic region of a chimeric receptor carrying the IL2R\u03b2 targeting signal resulted in a decreased degradation rate. When cells expressing IL2R\u03b2 were treated either by proteasome or lysosome inhibitors, a significant decrease in receptor degradation was observed. Our data show that ubiquitination is required for the sorting of IL2R\u03b2 toward degradation. They also indicate that impairment of proteasome function might more generally affect intracellular routing.</jats:p>",
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