DOI: 10.1091/mbc.12.3.539. The Nuclear Export Receptor Xpo1p Forms Distinct Complexes with NES Transport Substrates and the Yeast Ran Binding Protein 1 (Yrb1p)

The Nuclear Export Receptor Xpo1p Forms Distinct Complexes with NES Transport Substrates and the Yeast Ran Binding Protein 1 (Yrb1p)

10.1091/mbc.12.3.539

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Xpo1p (Crm1p) is the nuclear export receptor for proteins containing a leucine-rich nuclear export signal (NES). Xpo1p, the NES-containing protein, and GTP-bound Ran form a complex in the nucleus that translocates across the nuclear pore. We have identified Yrb1p as the major Xpo1p-binding protein in Saccharomyces cerevisiae extracts in the presence of GTP-bound Gsp1p (yeast Ran). Yrb1p is cytoplasmic at steady-state but shuttles continuously between the cytoplasm and the nucleus. Nuclear import of Yrb1p is mediated by two separate nuclear targeting signals. Export from the nucleus requires Xpo1p, but Yrb1p does not contain a leucine-rich NES. Instead, the interaction of Yrb1p with Xpo1p is mediated by Gsp1p-GTP. This novel type of export complex requires the acidic C-terminus of Gsp1p, which is dispensable for the binding to importin β-like transport receptors. A similar complex with Xpo1p and Gsp1p-GTP can be formed by Yrb2p, a relative of Yrb1p predominantly located in the nucleus. Yrb1p also functions as a disassembly factor for NES/Xpo1p/Gsp1p-GTP complexes by displacing the NES protein from Xpo1p/Gsp1p. This Yrb1p/Xpo1p/Gsp1p complex is then completely dissociated after GTP hydrolysis catalyzed by the cytoplasmic GTPase activating protein Rna1p.

Bibliography

Maurer, P., Redd, M., Solsbacher, J., Bischoff, F. R., Greiner, M., Podtelejnikov, A. V., Mann, M., Stade, K., Weis, K., & Schlenstedt, G. (2001). The Nuclear Export Receptor Xpo1p Forms Distinct Complexes with NES Transport Substrates and the Yeast Ran Binding Protein 1 (Yrb1p). Molecular Biology of the Cell, 12(3), 539â549.

Authors 10

Patrick Maurer (first)
Michael Redd (additional)
Jens Solsbacher (additional)
F. Ralf Bischoff (additional)
Markus Greiner (additional)
Alexandre V. Podtelejnikov (additional)
Matthias Mann (additional)
Katrin Stade (additional)
Karsten Weis (additional)
Gabriel Schlenstedt (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 8:09 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:44 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:35 p.m.)
Issued	24 years, 5 months ago (March 1, 2001)
Published	24 years, 5 months ago (March 1, 2001)
Published Print	24 years, 5 months ago (March 1, 2001)

Funders 0

None

BibTeX

@article{Maurer_2001, title={The Nuclear Export Receptor Xpo1p Forms Distinct Complexes with NES Transport Substrates and the Yeast Ran Binding Protein 1 (Yrb1p)}, volume={12}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.12.3.539}, DOI={10.1091/mbc.12.3.539}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Maurer, Patrick and Redd, Michael and Solsbacher, Jens and Bischoff, F. Ralf and Greiner, Markus and Podtelejnikov, Alexandre V. and Mann, Matthias and Stade, Katrin and Weis, Karsten and Schlenstedt, Gabriel}, editor={Pringle, John}, year={2001}, month=mar, pages={539–549} }

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