DOI: 10.1091/mbc.12.10.3242. Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval

Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval

10.1091/mbc.12.10.3242

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Endosome-to-Golgi retrieval of the carboxypeptidase Y receptor Vps10p is mediated by a recently discovered membrane coat complex termed retromer. Retromer comprises five conserved proteins: Vps35p, Vps29p, Vps5p, Vps17p, and Vps26p. Vps35p recognizes cargo molecules such as Vps10p and interacts strongly with Vps29p. Vps5p forms a subcomplex with Vps17p and has been proposed to play a structural role by self-assembling into large multimeric structures. The function of Vps26p is currently unknown. We have investigated the role that Vps26p plays in retromer-mediated endosome-to-Golgi transport by analyzing dominant negative alleles of Vps26p. These mutants have identified a crucial region of Vps26p that plays an important role in its function. Functional domains of Vps26p have been investigated by the creation of yeast-mouse hybrid molecules in which domains of Vps26p have been replaced by the similar domain in the protein encoded by the mouse VPS26 gene, Hβ58. These domain swap experiments have shown that Vps26p promotes the interactions between the cargo-selective component Vps35p and the structural components Vps5p/Vps17p.

Bibliography

Reddy, J. V., & Seaman, M. N. J. (2001). Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval. Molecular Biology of the Cell, 12(10), 3242â3256.

Authors 2

Jonathan V. Reddy (first)
Matthew N.J. Seaman (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 8:03 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:43 p.m.)
Indexed	3 months, 1 week ago (May 24, 2025, 8:22 a.m.)
Issued	23 years, 11 months ago (Oct. 1, 2001)
Published	23 years, 11 months ago (Oct. 1, 2001)
Published Print	23 years, 11 months ago (Oct. 1, 2001)

Funders 0

None

BibTeX

@article{Reddy_2001, title={Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval}, volume={12}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.12.10.3242}, DOI={10.1091/mbc.12.10.3242}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Reddy, Jonathan V. and Seaman, Matthew N.J.}, editor={Malhotra, Vivek}, year={2001}, month=oct, pages={3242–3256} }

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