DOI: 10.1091/mbc.11.9.3137. Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome

Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome–Lysosome Fusion

10.1091/mbc.11.9.3137

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Protein traffic from the cell surface or thetrans-Golgi network reaches the lysosome via a series of endosomal compartments. One of the last steps in the endocytic pathway is the fusion of late endosomes with lysosomes. This process has been reconstituted in vitro and has been shown to require NSF, α and γ SNAP, and a Rab GTPase based on inhibition by Rab GDI. InSaccharomyces cerevisiae, fusion events to the lysosome-like vacuole are mediated by the syntaxin protein Vam3p, which is localized to the vacuolar membrane. In an effort to identify the molecular machinery that controls fusion events to the lysosome, we searched for mammalian homologues of Vam3p. One such candidate is syntaxin 7. Here we show that syntaxin 7 is concentrated in late endosomes and lysosomes. Coimmunoprecipitation experiments show that syntaxin 7 is associated with the endosomal v-SNARE Vamp 8, which partially colocalizes with syntaxin 7. Importantly, we show that syntaxin 7 is specifically required for the fusion of late endosomes with lysosomes in vitro, resulting in a hybrid organelle. Together, these data identify a SNARE complex that functions in the late endocytic system of animal cells.

Bibliography

Mullock, B. M., Smith, C. W., Ihrke, G., Bright, N. A., Lindsay, M., Parkinson, E. J., Brooks, D. A., Parton, R. G., James, D. E., Luzio, J. P., & Piper, R. C. (2000). Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late EndosomeâLysosome Fusion. Molecular Biology of the Cell, 11(9), 3137â3153.

Authors 11

Barbara M. Mullock (first)
Chez W. Smith (additional)
Gudrun Ihrke (additional)
Nicholas A. Bright (additional)
Margaret Lindsay (additional)
Emma J. Parkinson (additional)
Doug A. Brooks (additional)
Robert G. Parton (additional)
David E. James (additional)
J. Paul Luzio (additional)
Robert C. Piper (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:59 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:42 p.m.)
Indexed	1 week, 4 days ago (Aug. 19, 2025, 6:55 a.m.)
Issued	24 years, 11 months ago (Sept. 1, 2000)
Published	24 years, 11 months ago (Sept. 1, 2000)
Published Print	24 years, 11 months ago (Sept. 1, 2000)

Funders 0

None

BibTeX

@article{Mullock_2000, title={Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome–Lysosome Fusion}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.9.3137}, DOI={10.1091/mbc.11.9.3137}, number={9}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Mullock, Barbara M. and Smith, Chez W. and Ihrke, Gudrun and Bright, Nicholas A. and Lindsay, Margaret and Parkinson, Emma J. and Brooks, Doug A. and Parton, Robert G. and James, David E. and Luzio, J. Paul and Piper, Robert C.}, editor={Pelham, Hugh R.B.}, year={2000}, month=sep, pages={3137–3153} }

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