DOI: 10.1091/mbc.11.8.2821. Cell Cycle-Dependent Expression of Mammalian E2-C Regulated by the Anaphase-Promoting Complex/Cyclosome

Cell Cycle-Dependent Expression of Mammalian E2-C Regulated by the Anaphase-Promoting Complex/Cyclosome

10.1091/mbc.11.8.2821

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Progression through mitosis requires the precisely timed ubiquitin-dependent degradation of specific substrates. E2-C is a ubiquitin-conjugating enzyme that plays a critical role with anaphase-promoting complex/cyclosome (APC/C) in progression of and exit from M phase. Here we report that mammalian E2-C is expressed in late G2/M phase and is degraded as cells exit from M phase. The mammalian E2-C shows an autoubiquitinating activity leading to covalent conjugation to itself with several ubiquitins. The ubiquitination of E2-C is strongly enhanced by APC/C, resulting in the formation of a polyubiquitin chain. The polyubiquitination of mammalian E2-C occurs only when cells exit from M phase. Furthermore, mammalian E2-C contains two putative destruction boxes that are believed to act as recognition motifs for APC/C. The mutation of this motif reduced the polyubiquitination of mammalian E2-C, resulting in its stabilization. These results suggest that mammalian E2-C is itself a substrate of the APC/C-dependent proteolysis machinery, and that the periodic expression of mammalian E2-C may be a novel autoregulatory system for the control of the APC/C activity and its substrate specificity.

Bibliography

Yamanaka, A., Hatakeyama, S., Kominami, K., Kitagawa, M., Matsumoto, M., & Nakayama, K. (2000). Cell Cycle-Dependent Expression of Mammalian E2-C Regulated by the Anaphase-Promoting Complex/Cyclosome. Molecular Biology of the Cell, 11(8), 2821â2831.

Authors 6

Atsushi Yamanaka (first)
Shigetsugu Hatakeyama (additional)
Kin-ichiro Kominami (additional)
Masatoshi Kitagawa (additional)
Masaki Matsumoto (additional)
Kei-ichi Nakayama (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:57 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:42 p.m.)
Indexed	1 year, 10 months ago (Oct. 16, 2023, 2:27 p.m.)
Issued	25 years ago (Aug. 1, 2000)
Published	25 years ago (Aug. 1, 2000)
Published Print	25 years ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Yamanaka_2000, title={Cell Cycle-Dependent Expression of Mammalian E2-C Regulated by the Anaphase-Promoting Complex/Cyclosome}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.8.2821}, DOI={10.1091/mbc.11.8.2821}, number={8}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Yamanaka, Atsushi and Hatakeyama, Shigetsugu and Kominami, Kin-ichiro and Kitagawa, Masatoshi and Matsumoto, Masaki and Nakayama, Kei-ichi}, editor={Solomon, Mark J.}, year={2000}, month=aug, pages={2821–2831} }

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