DOI: 10.1091/mbc.11.7.2315. The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination

The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination

10.1091/mbc.11.7.2315

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin ligase that mediates mitotic progression. Here, we provide evidence that theSaccharomyces cerevisiae RING-H2 finger protein Apc11 defines the minimal ubiquitin ligase activity of the APC. We found that the integrity of the Apc11p RING-H2 finger was essential for budding yeast cell viability, Using purified, recombinant proteins we showed that Apc11p interacted directly with the Ubc4 ubiquitin conjugating enzyme (E2). Furthermore, purified Apc11p was capable of mediating E1- and E2-dependent ubiquitination of protein substrates, including Clb2p, in vitro. The ability of Apc11p to act as an E3 was dependent on the integrity of the RING-H2 finger, but did not require the presence of the cullin-like APC subunit Apc2p. We suggest that Apc11p is responsible for recruiting E2s to the APC and for mediating the subsequent transfer of ubiquitin to APC substrates in vivo.

Bibliography

Leverson, J. D., Joazeiro, C. A. P., Page, A. M., Huang, H., Hieter, P., & Hunter, T. (2000). The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination. Molecular Biology of the Cell, 11(7), 2315â2325.

Authors 6

Joel D. Leverson (first)
Claudio A.P. Joazeiro (additional)
Andrew M. Page (additional)
Han-kuei Huang (additional)
Philip Hieter (additional)
Tony Hunter (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:55 p.m.)
Deposited	5 years, 1 month ago (July 29, 2020, 11:31 a.m.)
Indexed	4 months ago (April 29, 2025, 9:31 a.m.)
Issued	25 years, 1 month ago (July 1, 2000)
Published	25 years, 1 month ago (July 1, 2000)
Published Print	25 years, 1 month ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Leverson_2000, title={The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.7.2315}, DOI={10.1091/mbc.11.7.2315}, number={7}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Leverson, Joel D. and Joazeiro, Claudio A.P. and Page, Andrew M. and Huang, Han-kuei and Hieter, Philip and Hunter, Tony}, editor={Kirschner, Marc W.}, year={2000}, month=jul, pages={2315–2325} }

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