DOI: 10.1091/mbc.11.3.983. Sec24p and Iss1p Function Interchangeably in Transport Vesicle Formation from the Endoplasmic Reticulum in<i>Saccharomyces cerevisiae</i>

Sec24p and Iss1p Function Interchangeably in Transport Vesicle Formation from the Endoplasmic Reticulum inSaccharomyces cerevisiae

10.1091/mbc.11.3.983

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The Sec23p/Sec24p complex functions as a component of the COPII coat in vesicle transport from the endoplasmic reticulum. Here we characterize Saccharomyces cerevisiae SEC24, which encodes a protein of 926 amino acids (YIL109C), and a close homologue, ISS1 (YNL049C), which is 55% identical to SEC24. SEC24 is essential for vesicular transport in vivo because depletion of Sec24p is lethal, causing exaggeration of the endoplasmic reticulum and a block in the maturation of carboxypeptidase Y. Overproduction of Sec24p suppressed the temperature sensitivity of sec23-2, and overproduction of both Sec24p and Sec23p suppressed the temperature sensitivity of sec16-2. SEC24 gene disruption could be complemented by overexpression ofISS1, indicating functional redundancy between the two homologous proteins. Deletion of ISS1 had no significant effect on growth or secretion; however, iss1Δ mutants were found to be synthetically lethal with mutations in the v-SNARE genes SEC22 and BET1. Moreover, overexpression of ISS1 could suppress mutations inSEC22. These genetic interactions suggest that Iss1p may be specialized for the packaging or the function of COPII v-SNAREs. Iss1p tagged with His6at its C terminus copurified with Sec23p. Pure Sec23p/Iss1p could replace Sec23p/Sec24p in the packaging of a soluble cargo molecule (α-factor) and v-SNAREs (Sec22p and Bet1p) into COPII vesicles. Abundant proteins in the purified vesicles produced with Sec23p/Iss1p were indistinguishable from those in the regular COPII vesicles produced with Sec23p/Sec24p.

Bibliography

Kurihara, T., Hamamoto, S., Gimeno, R. E., Kaiser, C. A., Schekman, R., & Yoshihisa, T. (2000). Sec24p and Iss1p Function Interchangeably in Transport Vesicle Formation from the Endoplasmic Reticulum inSaccharomyces cerevisiae. Molecular Biology of the Cell, 11(3), 983â998.

Authors 6

Tatsuo Kurihara (first)
Susan Hamamoto (additional)
Ruth E. Gimeno (additional)
Chris A. Kaiser (additional)
Randy Schekman (additional)
Tohru Yoshihisa (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:48 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:41 p.m.)
Indexed	1 year, 2 months ago (June 27, 2024, 3:13 a.m.)
Issued	25 years, 6 months ago (March 1, 2000)
Published	25 years, 6 months ago (March 1, 2000)
Published Print	25 years, 6 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Kurihara_2000, title={Sec24p and Iss1p Function Interchangeably in Transport Vesicle Formation from the Endoplasmic Reticulum inSaccharomyces cerevisiae}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.3.983}, DOI={10.1091/mbc.11.3.983}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Kurihara, Tatsuo and Hamamoto, Susan and Gimeno, Ruth E. and Kaiser, Chris A. and Schekman, Randy and Yoshihisa, Tohru}, editor={Bonifacino, Juan}, year={2000}, month=mar, pages={983–998} }

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        2000,
        3
      ]
    ]
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  "journal-issue": {
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    "published-print": {
      "date-parts": [
        [
          2000,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.11.3.983"
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  "URL": "http://dx.doi.org/10.1091/mbc.11.3.983",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        2000,
        3
      ]
    ]
  }
}