DOI: 10.1091/mbc.11.2.613. <i>VPS21</i>Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment

VPS21Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment

10.1091/mbc.11.2.613

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Mutations in the VPS (vacuolar protein sorting) genes of Saccharomyces cerevisiae have been used to define the trafficking steps that soluble vacuolar hydrolases take en route from the late Golgi to the vacuole. The class DVPS genes include VPS21,PEP12, and VPS45, which appear to encode components of a membrane fusion complex involved in Golgi-to-endosome transport. Vps21p is a member of the Rab family of small Ras-like GTPases and shows strong homology to the mammalian Rab5 protein, which is involved in endocytosis and the homotypic fusion of early endosomes. Although Rab5 and Vps21p appear homologous at the sequence level, it has not been clear if the functions of these two Rabs are similar. We find that Vps21p is an endosomal protein that is involved in the delivery of vacuolar and endocytosed proteins to the vacuole. Vacuolar and endocytosed proteins accumulate in distinct transport intermediates in cells that lack Vps21p function. Therefore, it appears that Vps21p is involved in two trafficking steps into the prevacuolar/late endosomal compartment.

Bibliography

Gerrard, S. R., Bryant, N. J., & Stevens, T. H. (2000). VPS21Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment. Molecular Biology of the Cell, 11(2), 613â626.

Authors 3

Sonja R. Gerrard (first)
Nia J. Bryant (additional)
Tom H. Stevens (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:47 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:41 p.m.)
Indexed	2 months ago (June 21, 2025, 10:23 a.m.)
Issued	25 years, 6 months ago (Feb. 1, 2000)
Published	25 years, 6 months ago (Feb. 1, 2000)
Published Print	25 years, 6 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{Gerrard_2000, title={VPS21Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.2.613}, DOI={10.1091/mbc.11.2.613}, number={2}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Gerrard, Sonja R. and Bryant, Nia J. and Stevens, Tom H.}, editor={Kaiser, Chris}, year={2000}, month=feb, pages={613–626} }

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