DOI: 10.1091/mbc.11.12.4241. Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes

Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes

10.1091/mbc.11.12.4241

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

We explored genomic expression patterns in the yeastSaccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. A large set of genes (∼ 900) showed a similar drastic response to almost all of these environmental changes. Additional features of the genomic responses were specialized for specific conditions. Promoter analysis and subsequent characterization of the responses of mutant strains implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators. Physiological themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell.

Bibliography

Gasch, A. P., Spellman, P. T., Kao, C. M., Carmel-Harel, O., Eisen, M. B., Storz, G., Botstein, D., & Brown, P. O. (2000). Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes. Molecular Biology of the Cell, 11(12), 4241â4257.

Authors 8

Audrey P. Gasch (first)
Paul T. Spellman (additional)
Camilla M. Kao (additional)
Orna Carmel-Harel (additional)
Michael B. Eisen (additional)
Gisela Storz (additional)
David Botstein (additional)
Patrick O. Brown (additional)

References 44 Referenced 3,934

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:44 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:40 p.m.)
Indexed	26 minutes ago (Aug. 27, 2025, 8:19 p.m.)
Issued	24 years, 8 months ago (Dec. 1, 2000)
Published	24 years, 8 months ago (Dec. 1, 2000)
Published Print	24 years, 8 months ago (Dec. 1, 2000)

Funders 0

None

BibTeX

@article{Gasch_2000, title={Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.12.4241}, DOI={10.1091/mbc.11.12.4241}, number={12}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Gasch, Audrey P. and Spellman, Paul T. and Kao, Camilla M. and Carmel-Harel, Orna and Eisen, Michael B. and Storz, Gisela and Botstein, David and Brown, Patrick O.}, editor={Silver, Pamela A.}, year={2000}, month=dec, pages={4241–4257} }

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