DOI: 10.1091/mbc.11.12.4131. Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands

Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands

10.1091/mbc.11.12.4131

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Occludin is an integral membrane protein with four transmembrane domains that is exclusively localized at tight junction (TJ) strands. Here, we describe the generation and analysis of mice carrying a null mutation in the occludin gene. Occludin −/− mice were born with no gross phenotype in the expected Mendelian ratios, but they showed significant postnatal growth retardation. Occludin −/− males produced no litters with wild-type females, whereas occludin −/− females produced litters normally when mated with wild-type males but did not suckle them. In occludin −/− mice, TJs themselves did not appear to be affected morphologically, and the barrier function of intestinal epithelium was normal as far as examined electrophysiologically. However, histological abnormalities were found in several tissues, i.e., chronic inflammation and hyperplasia of the gastric epithelium, calcification in the brain, testicular atrophy, loss of cytoplasmic granules in striated duct cells of the salivary gland, and thinning of the compact bone. These phenotypes suggested that the functions of TJs as well as occludin are more complex than previously supposed.

Bibliography

Saitou, M., Furuse, M., Sasaki, H., Schulzke, J.-D., Fromm, M., Takano, H., Noda, T., & Tsukita, S. (2000). Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands. Molecular Biology of the Cell, 11(12), 4131â4142.

Authors 8

Mitinori Saitou (first)
Mikio Furuse (additional)
Hiroyuki Sasaki (additional)
Jörg-Dieter Schulzke (additional)
Michael Fromm (additional)
Hiroshi Takano (additional)
Tetsuo Noda (additional)
Shoichiro Tsukita (additional)

References 34 Referenced 987

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:44 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:40 p.m.)
Indexed	1 day ago (Sept. 2, 2025, 6:45 a.m.)
Issued	24 years, 9 months ago (Dec. 1, 2000)
Published	24 years, 9 months ago (Dec. 1, 2000)
Published Print	24 years, 9 months ago (Dec. 1, 2000)

Funders 0

None

BibTeX

@article{Saitou_2000, title={Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.12.4131}, DOI={10.1091/mbc.11.12.4131}, number={12}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Saitou, Mitinori and Furuse, Mikio and Sasaki, Hiroyuki and Schulzke, Jörg-Dieter and Fromm, Michael and Takano, Hiroshi and Noda, Tetsuo and Tsukita, Shoichiro}, editor={Nelson, W. James}, year={2000}, month=dec, pages={4131–4142} }

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