DOI: 10.1091/mbc.11.11.3977. Membrane Potential-Driven Protein Import into Mitochondria

Membrane Potential-Driven Protein Import into Mitochondria

10.1091/mbc.11.11.3977

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The transport of preproteins into or across the mitochondrial inner membrane requires the membrane potential Δψ across this membrane. Two roles of Δψ in the import of cleavable preproteins have been described: an electrophoretic effect on the positively charged matrix-targeting sequences and the activation of the translocase subunit Tim23. We report the unexpected finding that deletion of a segment within the sorting sequence of cytochromeb2, which is located behind the matrix-targeting sequence, strongly influenced the Δψ-dependence of import. The differential Δψ-dependence was independent of the submitochondrial destination of the preprotein and was not attributable to the requirement for mitochondrial Hsp70 or Tim23. With a series of preprotein constructs, the net charge of the sorting sequence was altered, but the Δψ-dependence of import was not affected. These results suggested that the sorting sequence contributed to the import driving mechanism in a manner distinct from the two known roles of Δψ. Indeed, a charge-neutral amino acid exchange in the hydrophobic segment of the sorting sequence generated a preprotein with an even better import, i.e. one with lower Δψ-dependence than the wild-type preprotein. The sorting sequence functioned early in the import pathway since it strongly influenced the efficiency of translocation of the matrix-targeting sequence across the inner membrane. These results suggest a model whereby an electrophoretic effect of Δψ on the matrix-targeting sequence is complemented by an import-stimulating activity of the sorting sequence.

Bibliography

Geissler, A., Krimmer, T., BÃ¶mer, U., Guiard, B., Rassow, J., & Pfanner, N. (2000). Membrane Potential-Driven Protein Import into Mitochondria. Molecular Biology of the Cell, 11(11), 3977â3991.

Authors 6

Andreas Geissler (first)
Thomas Krimmer (additional)
Ulf Bömer (additional)
Bernard Guiard (additional)
Joachim Rassow (additional)
Nikolaus Pfanner (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:43 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:40 p.m.)
Indexed	1 hour, 9 minutes ago (Sept. 3, 2025, 1:18 a.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Geissler_2000, title={Membrane Potential-Driven Protein Import into Mitochondria: The Sorting Sequence of Cytochromeb2Modulates the Δψ-Dependence of Translocation of the Matrix-targeting Sequence}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.11.3977}, DOI={10.1091/mbc.11.11.3977}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Geissler, Andreas and Krimmer, Thomas and Bömer, Ulf and Guiard, Bernard and Rassow, Joachim and Pfanner, Nikolaus}, editor={Fox, Thomas D.}, year={2000}, month=nov, pages={3977–3991} }

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