DOI: 10.1091/mbc.11.11.3885. The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import

The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import

10.1091/mbc.11.11.3885

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The bidirectional nucleocytoplasmic transport of macromolecules is mediated by the nuclear pore complex (NPC) which, in yeast, is composed of ∼30 different proteins (nucleoporins). Pre-embedding immunogold-electron microscopy revealed that Nic96p, an essential yeast nucleoporin, is located about the cytoplasmic and the nuclear periphery of the central channel, and near or at the distal ring of the yeast NPC. Genetic approaches further implicated Nic96p in nuclear protein import. To more specifically explore the potential role of Nic96p in nuclear protein import, we performed a two-hybrid screen withNIC96 as the bait against a yeast genomic library to identify transport factors and/or nucleoporins involved in nuclear protein import interacting with Nic96p. By doing so, we identified the yeast nucleoporin Nup53p, which also exhibits multiple locations within the yeast NPC and colocalizes with Nic96p in all its locations. Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export.

Bibliography

Fahrenkrog, B., HÃ¼bner, W., Mandinova, A., PantÃ©, N., Keller, W., & Aebi, U. (2000). The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import. Molecular Biology of the Cell, 11(11), 3885â3896.

Authors 6

Birthe Fahrenkrog (first)
Wolfgang Hübner (additional)
Anna Mandinova (additional)
Nelly Panté (additional)
Walter Keller (additional)
Ueli Aebi (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:43 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:40 p.m.)
Indexed	1 year ago (Aug. 3, 2024, 1:36 p.m.)
Issued	24 years, 9 months ago (Nov. 1, 2000)
Published	24 years, 9 months ago (Nov. 1, 2000)
Published Print	24 years, 9 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Fahrenkrog_2000, title={The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.11.3885}, DOI={10.1091/mbc.11.11.3885}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Fahrenkrog, Birthe and Hübner, Wolfgang and Mandinova, Anna and Panté, Nelly and Keller, Walter and Aebi, Ueli}, editor={Silver, Pamela A.}, year={2000}, month=nov, pages={3885–3896} }

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