DOI: 10.1091/mbc.11.11.3791. A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex

A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex

10.1091/mbc.11.11.3791

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The microtubule motor cytoplasmic dynein performs multiple cellular functions; however, the regulation and targeting of the motor to different cargoes is not well understood. A biochemical interaction between the dynein intermediate chain subunit and the p150-Glued component of the dynein regulatory complex, dynactin, has supported the hypothesis that the intermediate chain is a key modulator of dynein attachment to cellular cargoes. In this report, we identify multiple intermediate chain polypeptides that cosediment with the 19S dynein complex and two differentially expressed transcripts derived from the single cytoplasmic dynein intermediate chain (Cdic) gene that differ in the 3′ untranslated region sequence. These results support previous observations of multiple Cdic gene products that may contribute to the specialization of dynein function. Most significantly, we provide genetic evidence that the interaction between the dynein intermediate chain and p150-Glued is functionally relevant. We use a genomic Cdic transgene to show that extra copies of the dynein intermediate chain gene act to suppress the rough eye phenotype of the mutant Glued1, a mutation in the p150-Glued subunit of dynactin. Furthermore, we show that the interaction between the dynein intermediate chain and p150-Glued is dependent on the dosage of the Cdic gene. This result suggests that the dynein intermediate chain may be a limiting component in the assembly of the dynein complex and that the regulation of the interaction between the dynein intermediate chain and dynactin is critical for dynein function.

Bibliography

Boylan, K., Serr, M., & Hays, T. (2000). A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex. Molecular Biology of the Cell, 11(11), 3791â3803.

Authors 3

Kristin Boylan (first)
Madeline Serr (additional)
Tom Hays (additional)

References 63 Referenced 30

10.1126/science.287.5461.2185 / Science by Adams M.D. (2000)
10.1016/0092-8674(95)90377-1 / Cell by Ashfar K. (1995)
10.1089/dna.1995.14.349 / DNA Cell Biol. by Benevolenskaya E.V. (1995)
{'key': 'B4', 'first-page': '165', 'volume': '146', 'author': 'Bowman A.B.', 'year': '1999', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. by Bowman A.B. (1999)
10.1016/0092-8674(90)90452-K / Cell by Carthew R.W. (1990)
10.1126/science.282.5396.2012 / Science by C. elegans Genome Sequencing Consortium (1998)
10.1242/dev.114.3.653 / Development by Cheung H.K. (1992)
10.1006/geno.1998.5665 / Genomics by Crackower M.A. (1999)
10.1242/jcs.109.7.1891 / J. Cell Sci. by Criswell P.S. (1996)
10.1083/jcb.127.6.1671 / J. Cell Biol. by Dillman J.F. (1994)
10.1006/bbrc.1997.6379 / Biochem. Biophys. Res. Commun. by Farshori P. (1997)
10.1093/genetics/142.3.865 / Genetics. by Gepner J. (1996)
10.1091/mbc.8.6.1035
10.1083/jcb.115.6.1639 / J. Cell Biol. by Gill S.R. (1991)
10.1146/annurev.ge.27.120193.001535 / Annu. Rev. Genet. by Goldstein L.S.B. (1993)
10.1083/jcb.150.2.F63 / J. Cell Biol. by Goldstein L.S.B. (2000)
10.1073/pnas.91.22.10752 / Proc. Natl. Acad. Sci. USA by Hazelrigg T. (1994)
10.1016/0076-6879(87)55014-5 / Methods Enzymol. by Henikoff S. (1987)
10.1016/S0074-7696(08)62168-3 / Int. Rev. Cytol. by Holleran E.A. (1998)
10.1083/jcb.135.6.1815 / J. Cell Biol. by Holleran E.A. (1996)
10.1074/jbc.274.20.14262 / J. Biol. Chem. by Huang C.-Y.F. (1999)
10.1091/mbc.9.7.1741
10.1074/jbc.270.48.28806 / J. Biol. Chem. by Karki S. (1995)
10.1016/S0955-0674(99)80006-4 / Curr. Opin. Cell Biol. by Karki S. (1999)
10.1038/71338 / Nat. Cell Biol. by King S.J. (2000)
10.1016/S0167-4889(00)00009-4 / Biochim. Biophys. Acta by King S.M. (2000)
10.1021/bi9810813 / Biochemistry by King S.M. (1998)
10.1074/jbc.271.32.19358 / J. Biol. Chem. by King S.M. (1996)
10.1074/jbc.270.19.11445 / J. Biol. Chem. by King S.M. (1995)
10.1016/S0021-9258(18)92989-3 / J. Biol. Chem. by King S.M. (1991)
10.1038/227680a0 / Nature by Laemmli U.K. (1970)
10.1083/jcb.126.6.1475 / J. Cell Biol. by Li M.-G. (1994)
10.1093/genetics/139.4.1631 / Genetics by Maduñeo E. (1995)
10.1083/jcb.131.2.411 / J. Cell Biol. by McGrail M. (1995)
10.1083/jcb.113.4.835 / J. Cell Biol. by Mitchell D.R. (1991)
10.1002/cm.970060510 / Cell Motil. Cytoskeleton by Mitchell D.R. (1986)
10.1002/bies.950180308 / BioEssays by Moore J.D. (1996)
10.1128/MCB.18.11.6816 / Mol. Cell. Biol. by Nurminsky D.I. (1998)
10.1091/mbc.6.6.685
10.1083/jcb.118.5.1133 / J. Cell Biol. by Paschal B.M. (1992)
10.1083/jcb.144.3.473 / J. Cell Biol. by Pazour G.J. (1999)
10.1074/jbc.271.3.1687 / J. Biol. Chem. by Pfister K.K. (1996)
10.1091/mbc.10.3.693
10.1016/S1097-2765(00)80456-6 / Mol. Cell by Puthalakath H. (1999)
10.1091/mbc.5.1.45
10.1093/genetics/147.4.1635 / Genetics by Sandrock T.M. (1997)
10.1073/pnas.74.12.5463 / Proc. Natl. Acad. Sci. USA by Sanger F. (1977)
10.1083/jcb.115.5.1309 / J. Cell Biol. by Schroer T.A. (1991)
10.1016/S0959-437X(96)80083-9 / Curr. Opin. Genet. Dev. by Seydoux G. (1996)
10.1091/mbc.8.10.2077
10.1073/pnas.82.6.1751 / Proc. Natl. Acad. Sci. USA by Swaroop A. (1985)
10.1073/pnas.84.18.6501 / Proc. Natl. Acad. Sci. USA by Swaroop A. (1987)
10.1016/S0092-8674(00)80800-4 / Cell by Tai A.W. (1999)
10.1073/pnas.88.8.3120 / Proc. Natl. Acad. Sci. USA by Tamkun J.W. (1991)
10.1016/0378-1119(88)90177-1 / Gene by Thummel C.S. (1988)
10.1073/pnas.76.9.4350 / Proc. Natl. Acad. Sci. USA by Towbin H. (1979)
10.1083/jcb.133.4.831 / J. Cell Biol. by Vaisberg E.A. (1996)
10.1038/285674a0 / Nature by Vaslet C.A. (1980)
10.1083/jcb.131.6.1507 / J. Cell Biol. by Vaughan K.T. (1995)
10.1073/pnas.92.5.1634 / Proc. Natl. Acad. Sci. USA by Waterman-Storer C.M. (1995)
10.1016/S0959-437X(97)80132-3 / Curr. Opin. Genet. Dev. by Wickens M. (1997)
10.1083/jcb.129.1.169 / J. Cell Biol. by Wilkerson C.G. (1995)
10.1091/mbc.9.12.3335

Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:43 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:40 p.m.)
Indexed	1 year, 3 months ago (May 6, 2024, 12:34 p.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Boylan_2000, title={A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.11.3791}, DOI={10.1091/mbc.11.11.3791}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Boylan, Kristin and Serr, Madeline and Hays, Tom}, editor={McIntosh, J. Richard}, year={2000}, month=nov, pages={3791–3803} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        5,
        6
      ]
    ],
    "date-time": "2024-05-06T16:34:47Z",
    "timestamp": 1715013287306
  },
  "reference-count": 63,
  "publisher": "American Society for Cell Biology (ASCB)",
  "issue": "11",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        11
      ]
    ]
  },
  "abstract": "<jats:p>The microtubule motor cytoplasmic dynein performs multiple cellular functions; however, the regulation and targeting of the motor to different cargoes is not well understood. A biochemical interaction between the dynein intermediate chain subunit and the p150-Glued component of the dynein regulatory complex, dynactin, has supported the hypothesis that the intermediate chain is a key modulator of dynein attachment to cellular cargoes. In this report, we identify multiple intermediate chain polypeptides that cosediment with the 19S dynein complex and two differentially expressed transcripts derived from the single cytoplasmic dynein intermediate chain (Cdic) gene that differ in the 3\u2032 untranslated region sequence. These results support previous observations of multiple Cdic gene products that may contribute to the specialization of dynein function. Most significantly, we provide genetic evidence that the interaction between the dynein intermediate chain and p150-Glued is functionally relevant. We use a genomic Cdic transgene to show that extra copies of the dynein intermediate chain gene act to suppress the rough eye phenotype of the mutant Glued<jats:sup>1</jats:sup>, a mutation in the p150-Glued subunit of dynactin. Furthermore, we show that the interaction between the dynein intermediate chain and p150-Glued is dependent on the dosage of the Cdic gene. This result suggests that the dynein intermediate chain may be a limiting component in the assembly of the dynein complex and that the regulation of the interaction between the dynein intermediate chain and dynactin is critical for dynein function.</jats:p>",
  "DOI": "10.1091/mbc.11.11.3791",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2013,
        7,
        2
      ]
    ],
    "date-time": "2013-07-02T23:43:02Z",
    "timestamp": 1372808582000
  },
  "page": "3791-3803",
  "source": "Crossref",
  "is-referenced-by-count": 30,
  "title": "A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex",
  "prefix": "10.1091",
  "volume": "11",
  "author": [
    {
      "given": "Kristin",
      "family": "Boylan",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Genetics, Cell Biology, and Development, University of Minnesota, St. Paul, Minnesota 55108"
        }
      ]
    },
    {
      "given": "Madeline",
      "family": "Serr",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Genetics, Cell Biology, and Development, University of Minnesota, St. Paul, Minnesota 55108"
        }
      ]
    },
    {
      "given": "Tom",
      "family": "Hays",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Genetics, Cell Biology, and Development, University of Minnesota, St. Paul, Minnesota 55108"
        }
      ]
    }
  ],
  "member": "1076",
  "reference": [
    {
      "key": "B1",
      "doi-asserted-by": "crossref",
      "first-page": "2185",
      "DOI": "10.1126/science.287.5461.2185",
      "volume": "287",
      "author": "Adams M.D.",
      "year": "2000",
      "journal-title": "Science"
    },
    {
      "key": "B2",
      "doi-asserted-by": "crossref",
      "first-page": "129",
      "DOI": "10.1016/0092-8674(95)90377-1",
      "volume": "81",
      "author": "Ashfar K.",
      "year": "1995",
      "journal-title": "Cell"
    },
    {
      "key": "B3",
      "doi-asserted-by": "crossref",
      "first-page": "349",
      "DOI": "10.1089/dna.1995.14.349",
      "volume": "14",
      "author": "Benevolenskaya E.V.",
      "year": "1995",
      "journal-title": "DNA Cell Biol."
    },
    {
      "key": "B4",
      "first-page": "165",
      "volume": "146",
      "author": "Bowman A.B.",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B5",
      "doi-asserted-by": "crossref",
      "first-page": "561",
      "DOI": "10.1016/0092-8674(90)90452-K",
      "volume": "63",
      "author": "Carthew R.W.",
      "year": "1990",
      "journal-title": "Cell"
    },
    {
      "key": "B6",
      "doi-asserted-by": "crossref",
      "first-page": "2012",
      "DOI": "10.1126/science.282.5396.2012",
      "volume": "282",
      "author": "C. elegans Genome Sequencing Consortium",
      "year": "1998",
      "journal-title": "Science"
    },
    {
      "key": "B7",
      "doi-asserted-by": "crossref",
      "first-page": "653",
      "DOI": "10.1242/dev.114.3.653",
      "volume": "114",
      "author": "Cheung H.K.",
      "year": "1992",
      "journal-title": "Development"
    },
    {
      "key": "B8",
      "doi-asserted-by": "crossref",
      "first-page": "257",
      "DOI": "10.1006/geno.1998.5665",
      "volume": "55",
      "author": "Crackower M.A.",
      "year": "1999",
      "journal-title": "Genomics"
    },
    {
      "key": "B9",
      "doi-asserted-by": "crossref",
      "first-page": "1891",
      "DOI": "10.1242/jcs.109.7.1891",
      "volume": "109",
      "author": "Criswell P.S.",
      "year": "1996",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "B11",
      "doi-asserted-by": "crossref",
      "first-page": "1671",
      "DOI": "10.1083/jcb.127.6.1671",
      "volume": "127",
      "author": "Dillman J.F.",
      "year": "1994",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B12",
      "doi-asserted-by": "crossref",
      "first-page": "810",
      "DOI": "10.1006/bbrc.1997.6379",
      "volume": "232",
      "author": "Farshori P.",
      "year": "1997",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "B13",
      "doi-asserted-by": "crossref",
      "first-page": "865",
      "DOI": "10.1093/genetics/142.3.865",
      "volume": "142",
      "author": "Gepner J.",
      "year": "1996",
      "journal-title": "Genetics."
    },
    {
      "key": "B14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.6.1035"
    },
    {
      "key": "B15",
      "doi-asserted-by": "crossref",
      "first-page": "1639",
      "DOI": "10.1083/jcb.115.6.1639",
      "volume": "115",
      "author": "Gill S.R.",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B16",
      "doi-asserted-by": "crossref",
      "first-page": "319",
      "DOI": "10.1146/annurev.ge.27.120193.001535",
      "volume": "27",
      "author": "Goldstein L.S.B.",
      "year": "1993",
      "journal-title": "Annu. Rev. Genet."
    },
    {
      "key": "B17",
      "doi-asserted-by": "crossref",
      "first-page": "F63",
      "DOI": "10.1083/jcb.150.2.F63",
      "volume": "150",
      "author": "Goldstein L.S.B.",
      "year": "2000",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B20",
      "doi-asserted-by": "crossref",
      "first-page": "10752",
      "DOI": "10.1073/pnas.91.22.10752",
      "volume": "91",
      "author": "Hazelrigg T.",
      "year": "1994",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B21",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1016/0076-6879(87)55014-5",
      "volume": "155",
      "author": "Henikoff S.",
      "year": "1987",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "B22",
      "doi-asserted-by": "crossref",
      "first-page": "69",
      "DOI": "10.1016/S0074-7696(08)62168-3",
      "volume": "182",
      "author": "Holleran E.A.",
      "year": "1998",
      "journal-title": "Int. Rev. Cytol."
    },
    {
      "key": "B23",
      "doi-asserted-by": "crossref",
      "first-page": "1815",
      "DOI": "10.1083/jcb.135.6.1815",
      "volume": "135",
      "author": "Holleran E.A.",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B24",
      "doi-asserted-by": "crossref",
      "first-page": "14262",
      "DOI": "10.1074/jbc.274.20.14262",
      "volume": "274",
      "author": "Huang C.-Y.F.",
      "year": "1999",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.9.7.1741"
    },
    {
      "key": "B27",
      "doi-asserted-by": "crossref",
      "first-page": "28806",
      "DOI": "10.1074/jbc.270.48.28806",
      "volume": "270",
      "author": "Karki S.",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B28",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1016/S0955-0674(99)80006-4",
      "volume": "11",
      "author": "Karki S.",
      "year": "1999",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "B29",
      "doi-asserted-by": "crossref",
      "first-page": "20",
      "DOI": "10.1038/71338",
      "volume": "2",
      "author": "King S.J.",
      "year": "2000",
      "journal-title": "Nat. Cell Biol."
    },
    {
      "key": "B30",
      "doi-asserted-by": "crossref",
      "first-page": "60",
      "DOI": "10.1016/S0167-4889(00)00009-4",
      "volume": "1496",
      "author": "King S.M.",
      "year": "2000",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "B31",
      "doi-asserted-by": "crossref",
      "first-page": "15033",
      "DOI": "10.1021/bi9810813",
      "volume": "37",
      "author": "King S.M.",
      "year": "1998",
      "journal-title": "Biochemistry"
    },
    {
      "key": "B32",
      "doi-asserted-by": "crossref",
      "first-page": "19358",
      "DOI": "10.1074/jbc.271.32.19358",
      "volume": "271",
      "author": "King S.M.",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B33",
      "doi-asserted-by": "crossref",
      "first-page": "11445",
      "DOI": "10.1074/jbc.270.19.11445",
      "volume": "270",
      "author": "King S.M.",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B34",
      "doi-asserted-by": "crossref",
      "first-page": "8401",
      "DOI": "10.1016/S0021-9258(18)92989-3",
      "volume": "266",
      "author": "King S.M.",
      "year": "1991",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B36",
      "doi-asserted-by": "crossref",
      "first-page": "680",
      "DOI": "10.1038/227680a0",
      "volume": "227",
      "author": "Laemmli U.K.",
      "year": "1970",
      "journal-title": "Nature"
    },
    {
      "key": "B37",
      "doi-asserted-by": "crossref",
      "first-page": "1475",
      "DOI": "10.1083/jcb.126.6.1475",
      "volume": "126",
      "author": "Li M.-G.",
      "year": "1994",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B39",
      "doi-asserted-by": "crossref",
      "first-page": "1631",
      "DOI": "10.1093/genetics/139.4.1631",
      "volume": "139",
      "author": "Madu\u00f1eo E.",
      "year": "1995",
      "journal-title": "Genetics"
    },
    {
      "key": "B40",
      "doi-asserted-by": "crossref",
      "first-page": "411",
      "DOI": "10.1083/jcb.131.2.411",
      "volume": "131",
      "author": "McGrail M.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B42",
      "doi-asserted-by": "crossref",
      "first-page": "835",
      "DOI": "10.1083/jcb.113.4.835",
      "volume": "113",
      "author": "Mitchell D.R.",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B43",
      "doi-asserted-by": "crossref",
      "first-page": "510",
      "DOI": "10.1002/cm.970060510",
      "volume": "6",
      "author": "Mitchell D.R.",
      "year": "1986",
      "journal-title": "Cell Motil. Cytoskeleton"
    },
    {
      "key": "B44",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1002/bies.950180308",
      "volume": "18",
      "author": "Moore J.D.",
      "year": "1996",
      "journal-title": "BioEssays"
    },
    {
      "key": "B45",
      "doi-asserted-by": "crossref",
      "first-page": "6816",
      "DOI": "10.1128/MCB.18.11.6816",
      "volume": "18",
      "author": "Nurminsky D.I.",
      "year": "1998",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "B47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.6.6.685"
    },
    {
      "key": "B48",
      "doi-asserted-by": "crossref",
      "first-page": "1133",
      "DOI": "10.1083/jcb.118.5.1133",
      "volume": "118",
      "author": "Paschal B.M.",
      "year": "1992",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B49",
      "doi-asserted-by": "crossref",
      "first-page": "473",
      "DOI": "10.1083/jcb.144.3.473",
      "volume": "144",
      "author": "Pazour G.J.",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B50",
      "doi-asserted-by": "crossref",
      "first-page": "1687",
      "DOI": "10.1074/jbc.271.3.1687",
      "volume": "271",
      "author": "Pfister K.K.",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.10.3.693"
    },
    {
      "key": "B52",
      "doi-asserted-by": "crossref",
      "first-page": "287",
      "DOI": "10.1016/S1097-2765(00)80456-6",
      "volume": "3",
      "author": "Puthalakath H.",
      "year": "1999",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "B53",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.5.1.45"
    },
    {
      "key": "B55",
      "doi-asserted-by": "crossref",
      "first-page": "1635",
      "DOI": "10.1093/genetics/147.4.1635",
      "volume": "147",
      "author": "Sandrock T.M.",
      "year": "1997",
      "journal-title": "Genetics"
    },
    {
      "key": "B56",
      "doi-asserted-by": "crossref",
      "first-page": "5463",
      "DOI": "10.1073/pnas.74.12.5463",
      "volume": "74",
      "author": "Sanger F.",
      "year": "1977",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B57",
      "doi-asserted-by": "crossref",
      "first-page": "1309",
      "DOI": "10.1083/jcb.115.5.1309",
      "volume": "115",
      "author": "Schroer T.A.",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B58",
      "doi-asserted-by": "crossref",
      "first-page": "555",
      "DOI": "10.1016/S0959-437X(96)80083-9",
      "volume": "6",
      "author": "Seydoux G.",
      "year": "1996",
      "journal-title": "Curr. Opin. Genet. Dev."
    },
    {
      "key": "B59",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.10.2077"
    },
    {
      "key": "B60",
      "doi-asserted-by": "crossref",
      "first-page": "1751",
      "DOI": "10.1073/pnas.82.6.1751",
      "volume": "82",
      "author": "Swaroop A.",
      "year": "1985",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B61",
      "doi-asserted-by": "crossref",
      "first-page": "6501",
      "DOI": "10.1073/pnas.84.18.6501",
      "volume": "84",
      "author": "Swaroop A.",
      "year": "1987",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B62",
      "doi-asserted-by": "crossref",
      "first-page": "877",
      "DOI": "10.1016/S0092-8674(00)80800-4",
      "volume": "97",
      "author": "Tai A.W.",
      "year": "1999",
      "journal-title": "Cell"
    },
    {
      "key": "B63",
      "doi-asserted-by": "crossref",
      "first-page": "3120",
      "DOI": "10.1073/pnas.88.8.3120",
      "volume": "88",
      "author": "Tamkun J.W.",
      "year": "1991",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B64",
      "doi-asserted-by": "crossref",
      "first-page": "445",
      "DOI": "10.1016/0378-1119(88)90177-1",
      "volume": "74",
      "author": "Thummel C.S.",
      "year": "1988",
      "journal-title": "Gene"
    },
    {
      "key": "B65",
      "doi-asserted-by": "crossref",
      "first-page": "4350",
      "DOI": "10.1073/pnas.76.9.4350",
      "volume": "76",
      "author": "Towbin H.",
      "year": "1979",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B66",
      "doi-asserted-by": "crossref",
      "first-page": "831",
      "DOI": "10.1083/jcb.133.4.831",
      "volume": "133",
      "author": "Vaisberg E.A.",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B67",
      "doi-asserted-by": "crossref",
      "first-page": "674",
      "DOI": "10.1038/285674a0",
      "volume": "285",
      "author": "Vaslet C.A.",
      "year": "1980",
      "journal-title": "Nature"
    },
    {
      "key": "B68",
      "doi-asserted-by": "crossref",
      "first-page": "1507",
      "DOI": "10.1083/jcb.131.6.1507",
      "volume": "131",
      "author": "Vaughan K.T.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B69",
      "doi-asserted-by": "crossref",
      "first-page": "1634",
      "DOI": "10.1073/pnas.92.5.1634",
      "volume": "92",
      "author": "Waterman-Storer C.M.",
      "year": "1995",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B70",
      "doi-asserted-by": "crossref",
      "first-page": "220",
      "DOI": "10.1016/S0959-437X(97)80132-3",
      "volume": "7",
      "author": "Wickens M.",
      "year": "1997",
      "journal-title": "Curr. Opin. Genet. Dev."
    },
    {
      "key": "B71",
      "doi-asserted-by": "crossref",
      "first-page": "169",
      "DOI": "10.1083/jcb.129.1.169",
      "volume": "129",
      "author": "Wilkerson C.G.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B72",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.9.12.3335"
    }
  ],
  "container-title": "Molecular Biology of the Cell",
  "original-title": [],
  "language": "en",
  "deposited": {
    "date-parts": [
      [
        2022,
        2,
        27
      ]
    ],
    "date-time": "2022-02-27T02:40:15Z",
    "timestamp": 1645929615000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.molbiolcell.org/doi/10.1091/mbc.11.11.3791"
    }
  },
  "subtitle": [],
  "editor": [
    {
      "given": "J. Richard",
      "family": "McIntosh",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        11
      ]
    ]
  },
  "references-count": 63,
  "journal-issue": {
    "issue": "11",
    "published-print": {
      "date-parts": [
        [
          2000,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.11.11.3791"
  ],
  "URL": "http://dx.doi.org/10.1091/mbc.11.11.3791",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        2000,
        11
      ]
    ]
  }
}