DOI: 10.1091/mbc.11.10.3381. Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay

Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay

10.1091/mbc.11.10.3381

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The study of the nuclear pore in vertebrates would benefit from a strategy to directly identify new nucleoporins and interactions between those nucleoporins. We have developed a novel two-step “organelle trap” assay involving affinity selection and in vitro pore assembly. In the first step, soluble proteins derived from Xenopusegg extracts are applied to a column containing a ligand of interest. The bound proteins are then tagged by biotinylation and eluted. In the second step, potential nucleoporins are selected for by virtue of their ability to assemble into annulate lamellae, a cytoplasmic mimic of nuclear pores. The incorporated proteins are then recognized by their biotin tag. Here we use the lectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has been shown to directly bind three known nucleoporins from Xenopus extract, Nup62, Nup98, and Nup214, all of which containN-acetylglucosamine residues. Under reduced-stringency conditions, three additional proteins bind to WGA–Sepharose and are revealed by the organelle trap assay. We identified all three as partner nucleoporins. Two were discovered to be XenopusNup93 and Nup205. The third is a novel vertebrate nucleoporin, Nup188. This new vertebrate protein, Xenopus Nup188, exists in a complex with xNup93 and xNup205. The Nup93-Nup188-Nup205 complex does not bind directly to WGA but binds indirectly via theN-acetylglucosamine–modified nucleoporins. A gene encoding human Nup188 was also identified. The discovery of vertebrate Nup188, related to a yeast nucleoporin, and its novel protein–protein interactions illustrates the power of the two-step organelle trap assay and identifies new building blocks for constructing the nuclear pore.

Bibliography

Miller, B. R., Powers, M., Park, M., Fischer, W., & Forbes, D. J. (2000). Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay. Molecular Biology of the Cell, 11(10), 3381â3396.

Authors 5

Brian R. Miller (first)
Maureen Powers (additional)
Minkyu Park (additional)
Wolfgang Fischer (additional)
Douglass J. Forbes (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:41 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:40 p.m.)
Indexed	2 months ago (July 2, 2025, 2:35 p.m.)
Issued	24 years, 11 months ago (Oct. 1, 2000)
Published	24 years, 11 months ago (Oct. 1, 2000)
Published Print	24 years, 11 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{Miller_2000, title={Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.10.3381}, DOI={10.1091/mbc.11.10.3381}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Miller, Brian R. and Powers, Maureen and Park, Minkyu and Fischer, Wolfgang and Forbes, Douglass J.}, editor={Silver, Pamela A.}, year={2000}, month=oct, pages={3381–3396} }

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  "abstract": "<jats:p>The study of the nuclear pore in vertebrates would benefit from a strategy to directly identify new nucleoporins and interactions between those nucleoporins. We have developed a novel two-step \u201corganelle trap\u201d assay involving affinity selection and in vitro pore assembly. In the first step, soluble proteins derived from Xenopusegg extracts are applied to a column containing a ligand of interest. The bound proteins are then tagged by biotinylation and eluted. In the second step, potential nucleoporins are selected for by virtue of their ability to assemble into annulate lamellae, a cytoplasmic mimic of nuclear pores. The incorporated proteins are then recognized by their biotin tag. Here we use the lectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has been shown to directly bind three known nucleoporins from Xenopus extract, Nup62, Nup98, and Nup214, all of which containN-acetylglucosamine residues. Under reduced-stringency conditions, three additional proteins bind to WGA\u2013Sepharose and are revealed by the organelle trap assay. We identified all three as partner nucleoporins. Two were discovered to be XenopusNup93 and Nup205. The third is a novel vertebrate nucleoporin, Nup188. This new vertebrate protein, Xenopus Nup188, exists in a complex with xNup93 and xNup205. The Nup93-Nup188-Nup205 complex does not bind directly to WGA but binds indirectly via theN-acetylglucosamine\u2013modified nucleoporins. A gene encoding human Nup188 was also identified. The discovery of vertebrate Nup188, related to a yeast nucleoporin, and its novel protein\u2013protein interactions illustrates the power of the two-step organelle trap assay and identifies new building blocks for constructing the nuclear pore.</jats:p>",
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