DOI: 10.1091/mbc.11.1.1. Regulation of Mitotic Inhibitor Mik1 Helps to Enforce the DNA Damage Checkpoint

Regulation of Mitotic Inhibitor Mik1 Helps to Enforce the DNA Damage Checkpoint

10.1091/mbc.11.1.1

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The protein kinase Chk1 enforces the DNA damage checkpoint. This checkpoint delays mitosis until damaged DNA is repaired. Chk1 regulates the activity and localization of Cdc25, the tyrosine phosphatase that activates the cdk Cdc2. Here we report that Mik1, a tyrosine kinase that inhibits Cdc2, is positively regulated by the DNA damage checkpoint. Mik1 is required for checkpoint response in strains that lack Cdc25. Long-term DNA damage checkpoint arrest fails inΔmik1 cells. DNA damage increases Mik1 abundance in a Chk1-dependent manner. Ubiquitinated Mik1 accumulates in a proteasome mutant, which indicates that Mik1 normally has a short half-life. Thus, the DNA damage checkpoint might regulate Mik1 degradation. Mik1 protein and mRNA oscillate during the unperturbed cell cycle, with peak amounts detected around S phase. These data indicate that regulation of Mik1 abundance helps to couple mitotic onset to the completion of DNA replication and repair. Coordinated negative regulation of Cdc25 and positive regulation of Mik1 ensure the effective operation of the DNA damage checkpoint.

Bibliography

Baber-Furnari, B. A., Rhind, N., Boddy, M. N., Shanahan, P., Lopez-Girona, A., & Russell, P. (2000). Regulation of Mitotic Inhibitor Mik1 Helps to Enforce the DNA Damage Checkpoint. Molecular Biology of the Cell, 11(1), 1â11.

Authors 6

Beth A. Baber-Furnari (first)
Nick Rhind (additional)
Michael N. Boddy (additional)
Paul Shanahan (additional)
Antonia Lopez-Girona (additional)
Paul Russell (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:39 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 2:33 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 1:19 p.m.)
Issued	25 years, 8 months ago (Jan. 1, 2000)
Published	25 years, 8 months ago (Jan. 1, 2000)
Published Print	25 years, 8 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Baber_Furnari_2000, title={Regulation of Mitotic Inhibitor Mik1 Helps to Enforce the DNA Damage Checkpoint}, volume={11}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.11.1.1}, DOI={10.1091/mbc.11.1.1}, number={1}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Baber-Furnari, Beth A. and Rhind, Nick and Boddy, Michael N. and Shanahan, Paul and Lopez-Girona, Antonia and Russell, Paul}, editor={Yanagida, Mitsuhiro}, year={2000}, month=jan, pages={1–11} }

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  "abstract": "<jats:p> The protein kinase Chk1 enforces the DNA damage checkpoint. This checkpoint delays mitosis until damaged DNA is repaired. Chk1 regulates the activity and localization of Cdc25, the tyrosine phosphatase that activates the cdk Cdc2. Here we report that Mik1, a tyrosine kinase that inhibits Cdc2, is positively regulated by the DNA damage checkpoint. Mik1 is required for checkpoint response in strains that lack Cdc25. Long-term DNA damage checkpoint arrest fails in\u0394mik1 cells. DNA damage increases Mik1 abundance in a Chk1-dependent manner. Ubiquitinated Mik1 accumulates in a proteasome mutant, which indicates that Mik1 normally has a short half-life. Thus, the DNA damage checkpoint might regulate Mik1 degradation. Mik1 protein and mRNA oscillate during the unperturbed cell cycle, with peak amounts detected around S phase. These data indicate that regulation of Mik1 abundance helps to couple mitotic onset to the completion of DNA replication and repair. Coordinated negative regulation of Cdc25 and positive regulation of Mik1 ensure the effective operation of the DNA damage checkpoint. </jats:p>",
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