DOI: 10.1091/mbc.10.6.1973. The PDZ Domain of the LIM Protein Enigma Binds to β-Tropomyosin

The PDZ Domain of the LIM Protein Enigma Binds to β-Tropomyosin

10.1091/mbc.10.6.1973

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

PDZ and LIM domains are modular protein interaction motifs present in proteins with diverse functions. Enigma is representative of a family of proteins composed of a series of conserved PDZ and LIM domains. The LIM domains of Enigma and its most related family member, Enigma homology protein, bind to protein kinases, whereas the PDZ domains of Enigma and family member actin-associated LIM protein bind to actin filaments. Enigma localizes to actin filaments in fibroblasts via its PDZ domain, and actin-associated LIM protein binds to and colocalizes with the actin-binding protein α-actinin-2 at Z lines in skeletal muscle. We show that Enigma is present at the Z line in skeletal muscle and that the PDZ domain of Enigma binds to a skeletal muscle target, the actin-binding protein tropomyosin (skeletal β-TM). The interaction between Enigma and skeletal β-TM was specific for the PDZ domain of Enigma, was abolished by mutations in the PDZ domain, and required the PDZ-binding consensus sequence (Thr-Ser-Leu) at the extreme carboxyl terminus of skeletal β-TM. Enigma interacted with isoforms of tropomyosin expressed in C2C12 myotubes and formed an immunoprecipitable complex with skeletal β-TM in transfected cells. The association of Enigma with skeletal β-TM suggests a role for Enigma as an adapter protein that directs LIM-binding proteins to actin filaments of muscle cells.

Bibliography

Guy, P. M., Kenny, D. A., & Gill, G. N. (1999). The PDZ Domain of the LIM Protein Enigma Binds to Î²-Tropomyosin. Molecular Biology of the Cell, 10(6), 1973â1984.

Authors 3

Pamela M. Guy (first)
Daryn A. Kenny (additional)
Gordon N. Gill (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:32 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:39 p.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 7:31 a.m.)
Issued	26 years, 2 months ago (June 1, 1999)
Published	26 years, 2 months ago (June 1, 1999)
Published Print	26 years, 2 months ago (June 1, 1999)

Funders 0

None

BibTeX

@article{Guy_1999, title={The PDZ Domain of the LIM Protein Enigma Binds to β-Tropomyosin}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.6.1973}, DOI={10.1091/mbc.10.6.1973}, number={6}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Guy, Pamela M. and Kenny, Daryn A. and Gill, Gordon N.}, editor={Matsudaira, Paul T.}, year={1999}, month=jun, pages={1973–1984} }

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  "abstract": "<jats:p>PDZ and LIM domains are modular protein interaction motifs present in proteins with diverse functions. Enigma is representative of a family of proteins composed of a series of conserved PDZ and LIM domains. The LIM domains of Enigma and its most related family member, Enigma homology protein, bind to protein kinases, whereas the PDZ domains of Enigma and family member actin-associated LIM protein bind to actin filaments. Enigma localizes to actin filaments in fibroblasts via its PDZ domain, and actin-associated LIM protein binds to and colocalizes with the actin-binding protein \u03b1-actinin-2 at Z lines in skeletal muscle. We show that Enigma is present at the Z line in skeletal muscle and that the PDZ domain of Enigma binds to a skeletal muscle target, the actin-binding protein tropomyosin (skeletal \u03b2-TM). The interaction between Enigma and skeletal \u03b2-TM was specific for the PDZ domain of Enigma, was abolished by mutations in the PDZ domain, and required the PDZ-binding consensus sequence (Thr-Ser-Leu) at the extreme carboxyl terminus of skeletal \u03b2-TM. Enigma interacted with isoforms of tropomyosin expressed in C2C12 myotubes and formed an immunoprecipitable complex with skeletal \u03b2-TM in transfected cells. The association of Enigma with skeletal \u03b2-TM suggests a role for Enigma as an adapter protein that directs LIM-binding proteins to actin filaments of muscle cells.</jats:p>",
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    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1999,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.10.6.1973"
  ],
  "URL": "http://dx.doi.org/10.1091/mbc.10.6.1973",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        1999,
        6
      ]
    ]
  }
}