DOI: 10.1091/mbc.10.5.1367. Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy

Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy

10.1091/mbc.10.5.1367

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

In the yeast Saccharomyces cerevisiae, the Apg12p–Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this article, we investigated the function of Apg7p as an Apg12p-activating enzyme. Hemagglutinin-tagged Apg12p was coimmunoprecipitated with c-myc–tagged Apg7p. A two-hybrid experiment confirmed the interaction. The coimmunoprecipitation was sensitive to a thiol-reducing reagent. Furthermore, a thioester conjugate of Apg7p was detected in a lysate of cells overexpressing both Apg7p and Apg12p. These results indicated that Apg12p interacts with Apg7p via a thioester bond. Mutational analyses of Apg7p suggested that Cys507of Apg7p is an active site cysteine and that both the ATP-binding domain and the cysteine residue are essential for the conjugation of Apg7p with Apg12p to form the Apg12p–Apg5p conjugate. Cells expressing mutant Apg7ps, Apg7pG333A, or Apg7pC507Ashowed defects in autophagy and cytoplasm-to-vacuole targeting of aminopeptidase I. These results indicated that Apg7p functions as a novel protein-activating enzyme necessary for Apg12p–Apg5p conjugation.

Bibliography

Tanida, I., Mizushima, N., Kiyooka, M., Ohsumi, M., Ueno, T., Ohsumi, Y., & Kominami, E. (1999). Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy. Molecular Biology of the Cell, 10(5), 1367â1379.

Authors 7

Isei Tanida (first)
Noboru Mizushima (additional)
Miho Kiyooka (additional)
Mariko Ohsumi (additional)
Takashi Ueno (additional)
Yoshinori Ohsumi (additional)
Eiki Kominami (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:30 p.m.)
Deposited	2 years, 1 month ago (July 2, 2023, 5:06 p.m.)
Indexed	4 weeks ago (July 30, 2025, 11:02 a.m.)
Issued	26 years, 3 months ago (May 1, 1999)
Published	26 years, 3 months ago (May 1, 1999)
Published Print	26 years, 3 months ago (May 1, 1999)

Funders 0

None

BibTeX

@article{Tanida_1999, title={Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.5.1367}, DOI={10.1091/mbc.10.5.1367}, number={5}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Tanida, Isei and Mizushima, Noboru and Kiyooka, Miho and Ohsumi, Mariko and Ueno, Takashi and Ohsumi, Yoshinori and Kominami, Eiki}, editor={Schekman, Randy W.}, year={1999}, month=may, pages={1367–1379} }

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  "abstract": "<jats:p>In the yeast Saccharomyces cerevisiae, the Apg12p\u2013Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this article, we investigated the function of Apg7p as an Apg12p-activating enzyme. Hemagglutinin-tagged Apg12p was coimmunoprecipitated with c-myc\u2013tagged Apg7p. A two-hybrid experiment confirmed the interaction. The coimmunoprecipitation was sensitive to a thiol-reducing reagent. Furthermore, a thioester conjugate of Apg7p was detected in a lysate of cells overexpressing both Apg7p and Apg12p. These results indicated that Apg12p interacts with Apg7p via a thioester bond. Mutational analyses of Apg7p suggested that Cys<jats:sup>507</jats:sup>of Apg7p is an active site cysteine and that both the ATP-binding domain and the cysteine residue are essential for the conjugation of Apg7p with Apg12p to form the Apg12p\u2013Apg5p conjugate. Cells expressing mutant Apg7ps, Apg7p<jats:sup>G333A</jats:sup>, or Apg7p<jats:sup>C507A</jats:sup>showed defects in autophagy and cytoplasm-to-vacuole targeting of aminopeptidase I. These results indicated that Apg7p functions as a novel protein-activating enzyme necessary for Apg12p\u2013Apg5p conjugation.</jats:p>",
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