DOI: 10.1091/mbc.10.5.1325. Crosstalk between the Ras2p-controlled Mitogen-activated Protein Kinase and cAMP Pathways during Invasive Growth of<i>Saccharomyces cerevisiae</i>

Crosstalk between the Ras2p-controlled Mitogen-activated Protein Kinase and cAMP Pathways during Invasive Growth ofSaccharomyces cerevisiae

10.1091/mbc.10.5.1325

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The two highly conserved RAS genes of the budding yeast Saccharomyces cerevisiae are redundant for viability. Here we show that haploid invasive growth development depends on RAS2 but not RAS1. Ras1p is not sufficiently expressed to induce invasive growth. Ras2p activates invasive growth using either of two downstream signaling pathways, the filamentation MAPK (Cdc42p/Ste20p/MAPK) cascade or the cAMP-dependent protein kinase (Cyr1p/cAMP/PKA) pathway. This signal branch point can be uncoupled in cells expressing Ras2p mutant proteins that carry amino acid substitutions in the adenylyl cyclase interaction domain and therefore activate invasive growth solely dependent on the MAPK cascade. Both Ras2p-controlled signaling pathways stimulate expression of the filamentation response element-driven reporter gene depending on the transcription factors Ste12p and Tec1p, indicating a crosstalk between the MAPK and the cAMP signaling pathways in haploid cells during invasive growth.

Bibliography

MÃ¶sch, H.-U., KÃ¼bler, E., Krappmann, S., Fink, G. R., & Braus, G. H. (1999). Crosstalk between the Ras2p-controlled Mitogen-activated Protein Kinase and cAMP Pathways during Invasive Growth ofSaccharomyces cerevisiae. Molecular Biology of the Cell, 10(5), 1325â1335.

Authors 5

Hans-Ulrich Mösch (first)
Eric Kübler (additional)
Sven Krappmann (additional)
Gerald R. Fink (additional)
Gerhard H. Braus (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:30 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:38 p.m.)
Indexed	3 months ago (May 27, 2025, 1:39 p.m.)
Issued	26 years, 4 months ago (May 1, 1999)
Published	26 years, 4 months ago (May 1, 1999)
Published Print	26 years, 4 months ago (May 1, 1999)

Funders 0

None

BibTeX

@article{M_sch_1999, title={Crosstalk between the Ras2p-controlled Mitogen-activated Protein Kinase and cAMP Pathways during Invasive Growth ofSaccharomyces cerevisiae}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.5.1325}, DOI={10.1091/mbc.10.5.1325}, number={5}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Mösch, Hans-Ulrich and Kübler, Eric and Krappmann, Sven and Fink, Gerald R. and Braus, Gerhard H.}, editor={Botstein, David}, year={1999}, month=may, pages={1325–1335} }

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    "date-parts": [
      [
        1999,
        5
      ]
    ]
  },
  "references-count": 42,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          1999,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.10.5.1325"
  ],
  "URL": "http://dx.doi.org/10.1091/mbc.10.5.1325",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        1999,
        5
      ]
    ]
  }
}