DOI: 10.1091/mbc.10.4.875. Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment

Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment

10.1091/mbc.10.4.875

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Resident membrane proteins of the trans-Golgi network (TGN) ofSaccharomyces cerevisiae are selectively retrieved from a prevacuolar/late endosomal compartment. Proper cycling of the carboxypeptidase Y receptor Vps10p between the TGN and prevacuolar compartment depends on Vps35p, a hydrophilic peripheral membrane protein. In this study we use a temperature-sensitivevps35 allele to show that loss of Vps35p function rapidly leads to mislocalization of A-ALP, a model TGN membrane protein, to the vacuole. Vps35p is required for the prevacuolar compartment-to-TGN transport of both A-ALP and Vps10p. This was demonstrated by phenotypic analysis of vps35 mutant strains expressing A-ALP mutants lacking either the retrieval or static retention signals and by an assay for prevacuolar compartment-to-TGN transport. A novel vps35 allele was identified that was defective for retrieval of A-ALP but functional for retrieval of Vps10p. Moreover, several other vps35 alleles were identified with the opposite characteristics: they were defective for Vps10p retrieval but near normal for A-ALP localization. These data suggest a model in which distinct structural features within Vps35p are required for associating with the cytosolic domains of each cargo protein during the retrieval process.

Bibliography

Nothwehr, S. F., Bruinsma, P., & Strawn, L. A. (1999). Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment. Molecular Biology of the Cell, 10(4), 875â890.

Authors 3

Steven F. Nothwehr (first)
Paul Bruinsma (additional)
Lisa A. Strawn (additional)

References 62 Referenced 103

10.1074/jbc.273.19.11719 / J. Biol. Chem. by Abeliovich H. (1998)
10.1091/mbc.7.4.579
10.1016/0092-8674(95)90144-2 / Cell by Bednarek S.Y. (1995)
10.1091/mbc.5.9.1023
10.1007/BF00330984 / Mol. Gen. Genet. by Boeke J.D. (1984)
10.1083/jcb.139.1.23 / J. Cell Biol. by Brickner J.H. (1997)
10.1083/jcb.136.2.287 / J. Cell Biol. by Bryant N.J. (1997)
10.1101/gr.2.1.28 / PCR Methods Appl. by Cadwell R.C. (1992)
10.1091/mbc.6.9.1089
10.1002/j.1460-2075.1994.tb06817.x / EMBO J. by Chapman R.E. (1994)
10.1002/j.1460-2075.1994.tb06514.x / EMBO J. by Chapman R.E. (1994)
10.1083/jcb.119.6.1459 / J. Cell Biol. by Cooper A. (1992)
10.1083/jcb.133.3.529 / J. Cell Biol. by Cooper A.A. (1996)
10.1093/emboj/16.10.2769 / EMBO J. by Cowles C.R. (1997)
10.1083/jcb.111.2.369 / J. Cell Biol. by Dean N. (1990)
10.1016/S0092-8674(00)81171-X / Cell by Diaz E. (1998)
10.1083/jcb.106.3.617 / J. Cell Biol. by Duncan J.R. (1988)
10.1083/jcb.137.7.1511 / J. Cell Biol. by Fischer von Mollard G. (1997)
10.1146/annurev.ph.50.030188.002021 / Annu. Rev. Physiol. by Fuller R.S. (1988)
10.1083/jcb.127.3.653 / J. Cell Biol. by Gaynor E.C. (1994)
10.1093/emboj/17.1.113 / EMBO J. by Holthuis J.C.M. (1998)
10.1091/mbc.8.8.1529
10.1002/yea.320060502 / Yeast by Jones J.S. (1990)
10.1016/S0955-0674(97)80024-5 / Curr. Opin. Cell Biol. by Kirchhausen T. (1997)
10.1002/j.1460-2075.1989.tb08348.x / EMBO J. by Klionsky D.J. (1989)
10.1016/S0021-9258(18)54188-0 / J. Biol. Chem. by Köhrer K. (1993)
10.1146/annurev.bi.61.070192.001515 / Annu. Rev. Biochem. by Kornfeld S. (1992)
10.1016/0076-6879(87)54085-X / Methods Enzymol. by Kunkel T.A. (1987)
10.1016/0092-8674(94)90219-4 / Cell by Marcusson E.G. (1994)
10.1002/j.1460-2075.1994.tb06231.x / EMBO J. by Molloy S.S. (1994)
10.1002/j.1460-2075.1994.tb06294.x / EMBO J. by Nilsson T. (1994)
10.1128/MCB.16.6.2700 / Mol. Cell. Biol. by Nothwehr S.F. (1996)
10.1083/jcb.129.1.35 / J. Cell Biol. by Nothwehr S.F. (1995)
10.1242/jcs.110.9.1063 / J. Cell Sci. by Nothwehr S.F. (1997)
10.1083/jcb.121.6.1197 / J. Cell Biol. by Nothwehr S.F. (1993)
10.1091/mbc.3.4.415
10.1016/0092-8674(93)90479-A / Cell by Pelham H.R. (1993)
10.1083/jcb.131.3.603 / J. Cell Biol. by Piper R.C. (1995)
10.1091/mbc.3.12.1389
10.1083/jcb.113.3.527 / J. Cell Biol. by Redding K. (1991)
10.1083/jcb.126.5.1133 / J. Cell Biol. by Rexach M.F. (1994)
10.1083/jcb.125.3.573 / J. Cell Biol. by Riederer M.A. (1994)
10.1083/jcb.119.1.69 / J. Cell Biol. by Roberts C.J. (1992)
10.1016/0076-6879(91)94047-G / Methods Enzymol. by Roberts C.J. (1991)
10.1128/MCB.8.11.4936 / Mol. Cell. Biol. by Robinson J.S. (1988)
10.1016/0378-1119(87)90232-0 / Gene by Rose M.D. (1987)
10.1126/science.272.5259.227 / Science by Rothman J.E. (1996)
10.1016/0092-8674(86)90819-6 / Cell by Rothman J.H. (1986)
10.1002/j.1460-2075.1995.tb07240.x / EMBO J. by Schäfer W. (1995)
10.1146/annurev.biochem.66.1.511 / Annu. Rev. Biochem. by Schmid S.L. (1997)
10.1083/jcb.137.1.79 / J. Cell Biol. by Seaman M.N.J. (1997)
10.1083/jcb.142.3.665 / J. Cell Biol. by Seaman M.N.J. (1998)
10.1093/genetics/122.1.19 / Genetics by Sikorski R.S. (1989)
10.1016/0962-8924(93)90046-4 / Trends Cell Biol. by Stanely K.K. (1993)
10.1074/jbc.270.47.28397 / J. Biol. Chem. by Takahashi S. (1995)
10.1083/jcb.127.1.21 / J. Cell Biol. by Townsley F.M. (1994)
10.1083/jcb.119.4.773 / J. Cell Biol. by Vater C.A. (1992)
10.1002/j.1460-2075.1995.tb00179.x / EMBO J. by Voorhees P. (1995)
10.1083/jcb.140.3.577 / J. Cell Biol. by Voos W. (1998)
10.1016/S0092-8674(00)81420-8 / Cell by Wan L. (1998)
10.1083/jcb.122.6.1185 / J. Cell Biol. by Weisz O.A. (1993)
10.1091/mbc.3.12.1353

Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:28 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:38 p.m.)
Indexed	3 weeks ago (Aug. 2, 2025, 1:23 a.m.)
Issued	26 years, 4 months ago (April 1, 1999)
Published	26 years, 4 months ago (April 1, 1999)
Published Print	26 years, 4 months ago (April 1, 1999)

Funders 0

None

BibTeX

@article{Nothwehr_1999, title={Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.4.875}, DOI={10.1091/mbc.10.4.875}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Nothwehr, Steven F. and Bruinsma, Paul and Strawn, Lisa A.}, editor={Schekman, Randy W.}, year={1999}, month=apr, pages={875–890} }

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      "first-page": "28",
      "DOI": "10.1101/gr.2.1.28",
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    {
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      "year": "1997",
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      "year": "1993",
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      "DOI": "10.1083/jcb.113.3.527",
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      "author": "Redding K.",
      "year": "1991",
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      "doi-asserted-by": "crossref",
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      "year": "1994",
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