DOI: 10.1091/mbc.10.10.3289. Nascent Polypeptide–associated Complex Stimulates Protein Import into Yeast Mitochondria

Nascent Polypeptide–associated Complex Stimulates Protein Import into Yeast Mitochondria

10.1091/mbc.10.10.3289

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

To identify yeast cytosolic proteins that mediate targeting of precursor proteins to mitochondria, we developed an in vitro import system consisting of purified yeast mitochondria and a radiolabeled mitochondrial precursor protein whose C terminus was still attached to the ribosome. In this system, the N terminus of the nascent chain was translocated across both mitochondrial membranes, generating a translocation intermediate spanning both membranes. The nascent chain could then be completely chased into the mitochondrial matrix after release from the ribosome. Generation of this import intermediate was dependent on a mitochondrial membrane potential, mitochondrial surface proteins, and was stimulated by proteins that could be released from the ribosomes by high salt. The major salt-released stimulatory factor was yeast nascent polypeptide–associated complex (NAC). Purified NAC fully restored import of salt-washed ribosome-bound nascent chains by enhancing productive binding of the chains to mitochondria. We propose that ribosome-associated NAC facilitates recognition of nascent precursor chains by the mitochondrial import machinery.

Bibliography

FÃ¼nfschilling, U., & Rospert, S. (1999). Nascent Polypeptideâassociated Complex Stimulates Protein Import into Yeast Mitochondria. Molecular Biology of the Cell, 10(10), 3289â3299.

Authors 2

Ursula Fünfschilling (first)
Sabine Rospert (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:19 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:37 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 9:22 a.m.)
Issued	25 years, 10 months ago (Oct. 1, 1999)
Published	25 years, 10 months ago (Oct. 1, 1999)
Published Print	25 years, 10 months ago (Oct. 1, 1999)

Funders 0

None

BibTeX

@article{F_nfschilling_1999, title={Nascent Polypeptide–associated Complex Stimulates Protein Import into Yeast Mitochondria}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.10.3289}, DOI={10.1091/mbc.10.10.3289}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Fünfschilling, Ursula and Rospert, Sabine}, editor={Walter, Peter}, year={1999}, month=oct, pages={3289–3299} }

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