DOI: 10.1091/mbc.10.10.3171. TheSchizosaccharomyces pombe hst4+Gene Is aSIR2Homologue with Silencing and Centromeric Functions

TheSchizosaccharomyces pombe hst4+Gene Is aSIR2Homologue with Silencing and Centromeric Functions

10.1091/mbc.10.10.3171

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Although silencing is a significant form of transcriptional regulation, the functional and mechanistic limits of its conservation have not yet been established. We have identified theSchizosaccharomyces pombe hst4+gene as a member of the SIR2/HST silencing gene family that is defined in organisms ranging from bacteria to humans.hst4Δ mutants grow more slowly than wild-type cells and have abnormal morphology and fragmented DNA. Mutant strains show decreased silencing of reporter genes at both telomeres and centromeres. hst4+appears to be important for centromere function as well because mutants have elevated chromosome-loss rates and are sensitive to a microtubule-destabilizing drug. Consistent with a role in chromatin structure, Hst4p localizes to the nucleus and appears concentrated in the nucleolus.hst4Δ mutant phenotypes, including growth and silencing phenotypes, are similar to those of the Saccharomyces cerevisiae HSTs, and at a molecular level,hst4+is most similar toHST4. Furthermore, hst4+is a functional homologue of S. cerevisiae HST3 andHST4 in that overexpression ofhst4+rescues the temperature-sensitivity and telomeric silencing defects of an hst3Δ hst4Δdouble mutant. These results together demonstrate that aSIR-like silencing mechanism is conserved in the distantly related yeasts and is likely to be found in other organisms from prokaryotes to mammals.

Bibliography

Freeman-Cook, L. L., Sherman, J. M., Brachmann, C. B., Allshire, R. C., Boeke, J. D., & Pillus, L. (1999). TheSchizosaccharomyces pombe hst4+Gene Is aSIR2Homologue with Silencing and Centromeric Functions. Molecular Biology of the Cell, 10(10), 3171â3186.

Authors 6

Lisa L. Freeman-Cook (first)
Joyce M. Sherman (additional)
Carrie B. Brachmann (additional)
Robin C. Allshire (additional)
Jef D. Boeke (additional)
Lorraine Pillus (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:19 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:37 p.m.)
Indexed	1 year, 1 month ago (July 26, 2024, 3:58 p.m.)
Issued	25 years, 10 months ago (Oct. 1, 1999)
Published	25 years, 10 months ago (Oct. 1, 1999)
Published Print	25 years, 10 months ago (Oct. 1, 1999)

Funders 0

None

BibTeX

@article{Freeman_Cook_1999, title={TheSchizosaccharomyces pombe hst4+Gene Is aSIR2Homologue with Silencing and Centromeric Functions}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.10.3171}, DOI={10.1091/mbc.10.10.3171}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Freeman-Cook, Lisa L. and Sherman, Joyce M. and Brachmann, Carrie B. and Allshire, Robin C. and Boeke, Jef D. and Pillus, Lorraine}, editor={Gall, Joseph}, year={1999}, month=oct, pages={3171–3186} }

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  "abstract": "<jats:p>Although silencing is a significant form of transcriptional regulation, the functional and mechanistic limits of its conservation have not yet been established. We have identified theSchizosaccharomyces pombe hst4<jats:sup>+</jats:sup>gene as a member of the SIR2/HST silencing gene family that is defined in organisms ranging from bacteria to humans.hst4\u0394 mutants grow more slowly than wild-type cells and have abnormal morphology and fragmented DNA. Mutant strains show decreased silencing of reporter genes at both telomeres and centromeres. hst4<jats:sup>+</jats:sup>appears to be important for centromere function as well because mutants have elevated chromosome-loss rates and are sensitive to a microtubule-destabilizing drug. Consistent with a role in chromatin structure, Hst4p localizes to the nucleus and appears concentrated in the nucleolus.hst4\u0394 mutant phenotypes, including growth and silencing phenotypes, are similar to those of the Saccharomyces cerevisiae HSTs, and at a molecular level,hst4<jats:sup>+</jats:sup>is most similar toHST4. Furthermore, hst4<jats:sup>+</jats:sup>is a functional homologue of S. cerevisiae HST3 andHST4 in that overexpression ofhst4<jats:sup>+</jats:sup>rescues the temperature-sensitivity and telomeric silencing defects of an hst3\u0394 hst4\u0394double mutant. These results together demonstrate that aSIR-like silencing mechanism is conserved in the distantly related yeasts and is likely to be found in other organisms from prokaryotes to mammals.</jats:p>",
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