DOI: 10.1091/mbc.10.1.63. Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast

Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast

10.1091/mbc.10.1.63

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The yeast transport GTPase Ypt6p is dispensable for cell growth and secretion, but its lack results in temperature sensitivity and missorting of vacuolar carboxypeptidase Y. We previously identified four yeast genes (SYS1, 2, 3, and 5) that on high expression suppressed these phenotypic alterations.SYS3 encodes a 105-kDa protein with a predicted high α-helical content. It is related to a variety of mammalian Golgi-associated proteins and to the yeast Uso1p, an essential protein involved in docking of endoplasmic reticulum–derived vesicles to thecis-Golgi. Like Uso1p, Sys3p is predominatly cytosolic. According to gel chromatographic, two-hybrid, and chemical cross-linking analyses, Sys3p forms dimers and larger protein complexes. Its loss of function results in partial missorting of carboxypeptidase Y. Double disruptions of SYS3and YPT6 lead to a significant growth inhibition of the mutant cells, to a massive accumulation of 40- to 50-nm vesicles, to an aggravation of vacuolar protein missorting, and to a defect in α-pheromone processing apparently attributable to a perturbation of protease Kex2p cycling between the Golgi and a post-Golgi compartment. The results of this study suggest that Sys3p, like Ypt6p, acts in vesicular transport (presumably at a vesicle-docking stage) between an endosomal compartment and the most distal Golgi compartment.

Bibliography

Tsukada, M., Will, E., & Gallwitz, D. (1999). Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast. Molecular Biology of the Cell, 10(1), 63â75.

Authors 3

Miki Tsukada (first)
Elke Will (additional)
Dieter Gallwitz (additional)

References 43 Referenced 74

10.1093/nar/25.17.3389 / Nucleic Acids. Res. by Altschul S.F. (1995)
10.1002/j.1460-2075.1991.tb08010.x / EMBO J. by Becker J. (1991)
10.1093/emboj/17.8.2156 / EMBO J. by Cao X. (1998)
10.1091/mbc.6.9.1089
10.1083/jcb.133.3.529 / J. Cell Biol. by Cooper A.A. (1996)
10.1016/0092-8674(92)90194-H / Cell by Dalton S. (1992)
10.1128/MCB.11.2.872 / Mol. Cell. Biol. by Dascher C. (1991)
10.1093/protein/1.4.289 / Protein Eng. by Deleage G. (1987)
10.1074/jbc.271.14.8328 / J. Biol. Chem. by Erlich R. (1996)
10.1007/BF02603120 / J. Mol. Evol. by Feng D.F. (1987)
{'key': 'B11', 'first-page': '681', 'volume': '11', 'author': 'Geourjon C.', 'year': '1995', 'journal-title': 'Comput. Appl. Biosci.'} / Comput. Appl. Biosci. by Geourjon C. (1995)
10.1016/0022-2836(87)90292-0 / J. Mol. Biol. by Gibrat J.-F. (1987)
10.1016/0092-8674(93)90499-G / Cell by Harper J.W. (1993)
10.1016/0378-1119(89)90358-2 / Gene by Ho S.N. (1989)
10.1002/j.1460-2075.1994.tb06382.x / EMBO J. by Horazdovsky B.F. (1994)
10.1128/JB.153.1.163-168.1983 / J. Bacteriol. by Ito H. (1983)
10.1016/0092-8674(83)90070-3 / Cell by Julius D. (1983)
10.1016/S0968-0004(97)01150-X / Trends Biochem. Sci. by Lazar T. (1997)
10.1016/0014-5793(86)80917-6 / FEBS Lett. by Levin J.M. (1986)
10.1074/jbc.271.28.16813 / J. Biol. Chem. by Li B. (1996)
10.1073/pnas.92.11.5102 / Proc. Natl. Acad. Sci. USA by Linstedt A.D. (1995)
10.1091/mbc.4.7.679
10.1074/jbc.270.22.13503 / J. Biol. Chem. by Mu F.-T. (1995)
10.1083/jcb.113.2.245 / J. Cell Biol. by Nakajima H. (1991)
10.1016/S0092-8674(00)80225-1 / Cell by Nakamura N. (1997)
10.1016/S0021-9258(17)34040-1 / J. Biol. Chem. by Nothwehr S.F. (1994)
10.1016/S0955-0674(97)80025-7 / Curr. Opin. Cell Biol. by Novick P. (1997)
10.1128/MCB.11.6.2980 / Mol. Cell. Biol. by Ossig R. (1991)
10.1091/mbc.3.12.1389
10.1002/prot.340190108 / Proteins by Rost B. (1994)
10.1083/jcb.132.5.755 / J. Cell Biol. by Sapperstein S.K. (1996)
10.1073/pnas.92.2.522 / Proc. Natl. Acad. Sci. USA by Sapperstein S.K. (1995)
10.1074/jbc.273.35.22161 / J. Biol. Chem. by Schimmöller F. (1998)
10.1128/MCB.14.4.2564 / Mol. Cell. Biol. by Seelig H.P. (1994)
10.1006/bbrc.1994.1497 / Biochem. Biophys. Res. Commun. by Seog D.-H. (1994)
10.1038/28879 / Nature by Simonsen A. (1998)
10.1083/jcb.125.2.283 / J. Cell Biol. by Singer-Krüger B. (1994)
10.1083/jcb.140.5.1013 / J. Cell Biol. by Sönnichsen B. (1998)
10.1038/361736a0 / Nature by Strom M. (1993)
10.1242/jcs.109.10.2471 / J. Cell Sci. by Tsukada M. (1996)
10.1083/jcb.128.5.779 / J. Cell Biol. by Vida T.A. (1995)
10.1016/S0955-0674(98)80064-1 / Curr. Opin. Cell Biol. by Warren G. (1998)
10.1016/0962-8924(93)90031-U / Trends Cell Biol. by Wilsbach K. (1993)

Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:17 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:37 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 13, 2024, 1:44 p.m.)
Issued	26 years, 8 months ago (Jan. 1, 1999)
Published	26 years, 8 months ago (Jan. 1, 1999)
Published Print	26 years, 8 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Tsukada_1999, title={Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast}, volume={10}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.10.1.63}, DOI={10.1091/mbc.10.1.63}, number={1}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Tsukada, Miki and Will, Elke and Gallwitz, Dieter}, editor={Pfeffer, Suzanne R.}, year={1999}, month=jan, pages={63–75} }

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  "abstract": "<jats:p>The yeast transport GTPase Ypt6p is dispensable for cell growth and secretion, but its lack results in temperature sensitivity and missorting of vacuolar carboxypeptidase Y. We previously identified four yeast genes (SYS1, 2, 3, and 5) that on high expression suppressed these phenotypic alterations.SYS3 encodes a 105-kDa protein with a predicted high \u03b1-helical content. It is related to a variety of mammalian Golgi-associated proteins and to the yeast Uso1p, an essential protein involved in docking of endoplasmic reticulum\u2013derived vesicles to thecis-Golgi. Like Uso1p, Sys3p is predominatly cytosolic. According to gel chromatographic, two-hybrid, and chemical cross-linking analyses, Sys3p forms dimers and larger protein complexes. Its loss of function results in partial missorting of carboxypeptidase Y. Double disruptions of SYS3and YPT6 lead to a significant growth inhibition of the mutant cells, to a massive accumulation of 40- to 50-nm vesicles, to an aggravation of vacuolar protein missorting, and to a defect in \u03b1-pheromone processing apparently attributable to a perturbation of protease Kex2p cycling between the Golgi and a post-Golgi compartment. The results of this study suggest that Sys3p, like Ypt6p, acts in vesicular transport (presumably at a vesicle-docking stage) between an endosomal compartment and the most distal Golgi compartment.</jats:p>",
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      "first-page": "597",
      "DOI": "10.1016/0092-8674(92)90194-H",
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      "author": "Dalton S.",
      "year": "1992",
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    {
      "key": "B7",
      "doi-asserted-by": "crossref",
      "first-page": "872",
      "DOI": "10.1128/MCB.11.2.872",
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      "author": "Dascher C.",
      "year": "1991",
      "journal-title": "Mol. Cell. Biol."
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    {
      "key": "B8",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1093/protein/1.4.289",
      "volume": "1",
      "author": "Deleage G.",
      "year": "1987",
      "journal-title": "Protein Eng."
    },
    {
      "key": "B9",
      "doi-asserted-by": "crossref",
      "first-page": "8328",
      "DOI": "10.1074/jbc.271.14.8328",
      "volume": "271",
      "author": "Erlich R.",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "B10",
      "doi-asserted-by": "crossref",
      "first-page": "351",
      "DOI": "10.1007/BF02603120",
      "volume": "25",
      "author": "Feng D.F.",
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      "journal-title": "J. Mol. Evol."
    },
    {
      "key": "B11",
      "first-page": "681",
      "volume": "11",
      "author": "Geourjon C.",
      "year": "1995",
      "journal-title": "Comput. Appl. Biosci."
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    {
      "key": "B12",
      "doi-asserted-by": "crossref",
      "first-page": "425",
      "DOI": "10.1016/0022-2836(87)90292-0",
      "volume": "198",
      "author": "Gibrat J.-F.",
      "year": "1987",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "B13",
      "doi-asserted-by": "crossref",
      "first-page": "805",
      "DOI": "10.1016/0092-8674(93)90499-G",
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    {
      "key": "B16",
      "doi-asserted-by": "crossref",
      "first-page": "1297",
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      "DOI": "10.1128/JB.153.1.163-168.1983",
      "volume": "153",
      "author": "Ito H.",
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      "DOI": "10.1016/S0968-0004(97)01150-X",
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      "author": "Lazar T.",
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      "doi-asserted-by": "crossref",
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      "doi-asserted-by": "crossref",
      "first-page": "5102",
      "DOI": "10.1073/pnas.92.11.5102",
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      "year": "1995",
      "journal-title": "J. Biol. Chem."
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      "first-page": "245",
      "DOI": "10.1083/jcb.113.2.245",
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      "author": "Nakajima H.",
      "year": "1991",
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      "first-page": "445",
      "DOI": "10.1016/S0092-8674(00)80225-1",
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      "year": "1997",
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    {
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      "DOI": "10.1016/S0021-9258(17)34040-1",
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      "author": "Nothwehr S.F.",
      "year": "1994",
      "journal-title": "J. Biol. Chem."
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    {
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      "author": "Novick P.",
      "year": "1997",
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      "key": "B30",
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      "DOI": "10.1091/mbc.3.12.1389"
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      "key": "B33",
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      "year": "1996",
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      "DOI": "10.1074/jbc.273.35.22161",
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      "author": "Schimm\u00f6ller F.",
      "year": "1998",
      "journal-title": "J. Biol. Chem."
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    {
      "key": "B36",
      "doi-asserted-by": "crossref",
      "first-page": "2564",
      "DOI": "10.1128/MCB.14.4.2564",
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      "key": "B37",
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      "doi-asserted-by": "crossref",
      "first-page": "494",
      "DOI": "10.1038/28879",
      "volume": "394",
      "author": "Simonsen A.",
      "year": "1998",
      "journal-title": "Nature"
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    {
      "key": "B39",
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      "year": "1994",
      "journal-title": "J. Cell Biol."
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      "first-page": "1013",
      "DOI": "10.1083/jcb.140.5.1013",
      "volume": "140",
      "author": "S\u00f6nnichsen B.",
      "year": "1998",
      "journal-title": "J. Cell Biol."
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      "doi-asserted-by": "crossref",
      "first-page": "736",
      "DOI": "10.1038/361736a0",
      "volume": "361",
      "author": "Strom M.",
      "year": "1993",
      "journal-title": "Nature"
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    {
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      "DOI": "10.1242/jcs.109.10.2471",
      "volume": "109",
      "author": "Tsukada M.",
      "year": "1996",
      "journal-title": "J. Cell Sci."
    },
    {
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      "doi-asserted-by": "crossref",
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      "DOI": "10.1083/jcb.128.5.779",
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      "year": "1995",
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      "author": "Warren G.",
      "year": "1998",
      "journal-title": "Curr. Opin. Cell Biol."
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      "first-page": "426",
      "DOI": "10.1016/0962-8924(93)90031-U",
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      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1999,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.10.1.63"
  ],
  "URL": "http://dx.doi.org/10.1091/mbc.10.1.63",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        1999,
        1
      ]
    ]
  }
}