DOI: 10.1091/mbc.02-05-0074. Human MPS1 Kinase Is Required for Mitotic Arrest Induced by the Loss of CENP-E from Kinetochores

Human MPS1 Kinase Is Required for Mitotic Arrest Induced by the Loss of CENP-E from Kinetochores

10.1091/mbc.02-05-0074

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

We have determined that the previously identified dual-specificity protein kinase TTK is the human orthologue of the yeast MPS1 kinase. Yeast MPS1 (monopolar spindle) is required for spindle pole duplication and the spindle checkpoint. Consistent with the recently identified vertebrate MPS1 homologues, we found that hMPS1 is localized to centrosomes and kinetochores. In addition, hMPS1 is part of a growing list of kinetochore proteins that are localized to nuclear pores. hMPS1 is required by cells to arrest in mitosis in response to spindle defects and kinetochore defects resulting from the loss of the kinesin-like protein, CENP-E. The pattern of kinetochore localization of hMPS1 in CENP-E defective cells suggests that their interaction with the kinetochore is sensitive to microtubule occupancy rather than kinetochore tension. hMPS1 is required for MAD1, MAD2 but not hBUB1, hBUBR1 and hROD to bind to kinetochores. We localized the kinetochore targeting domain in hMPS1 and found that it can abrogate the mitotic checkpoint in a dominant negative manner. Last, hMPS1 was found to associate with the anaphase promoting complex, thus raising the possibility that its checkpoint functions extend beyond the kinetochore.

Bibliography

Liu, S.-T., Chan, G. K. T., Hittle, J. C., Fujii, G., Lees, E., & Yen, T. J. (2003). Human MPS1 Kinase Is Required for Mitotic Arrest Induced by the Loss of CENP-E from Kinetochores. Molecular Biology of the Cell, 14(4), 1638â1651.

Authors 6

Song-Tao Liu (first)
Gordon K.T. Chan (additional)
James C. Hittle (additional)
Gregory Fujii (additional)
Emma Lees (additional)
Tim J. Yen (additional)

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Dates

Type	When
Created	22 years, 4 months ago (April 9, 2003, 2:54 p.m.)
Deposited	2 years, 4 months ago (April 26, 2023, 1:09 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 15, 2024, 4:47 p.m.)
Issued	22 years, 4 months ago (April 1, 2003)
Published	22 years, 4 months ago (April 1, 2003)
Published Print	22 years, 4 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{Liu_2003, title={Human MPS1 Kinase Is Required for Mitotic Arrest Induced by the Loss of CENP-E from Kinetochores}, volume={14}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.02-05-0074}, DOI={10.1091/mbc.02-05-0074}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Liu, Song-Tao and Chan, Gordon K.T. and Hittle, James C. and Fujii, Gregory and Lees, Emma and Yen, Tim J.}, editor={Salmon, Ted}, year={2003}, month=apr, pages={1638–1651} }

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