DOI: 10.1091/mbc.01-12-0593. Structure of the Golgi and Distribution of Reporter Molecules at 20°C Reveals the Complexity of the Exit Compartments

Structure of the Golgi and Distribution of Reporter Molecules at 20°C Reveals the Complexity of the Exit Compartments

10.1091/mbc.01-12-0593

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Incubating cells at 20°C blocks transport out of the Golgi complex and amplifies the exit compartments. We have used the 20°C block, followed by EM tomography and serial section reconstruction, to study the structure of Golgi exit sites in NRK cells. The dominant feature of Golgi structure in temperature-blocked cells is the presence of large bulging domains on the three trans-most cisternae. These domains extend laterally from the stack and are continuous with “cisternal” domains that maintain normal thickness and alignment with the other stacked Golgi cisternae. The bulging domains do not resemble the perpendicularly extending tubules associated with the trans-cisternae of control cells. Such tubules are completely absent in temperature-blocked cells. The three cisternae with bulging domains can be identified as trans by their association with specialized ER and the presence of clathrin-coated buds on the trans-most cisterna only. Immunogold labeling and immunoblots show a significant degradation of a medial- and a trans-Golgi marker with no evidence for their redistribution within the Golgi or to other organelles. These data suggest that exit from the Golgi occurs directly from three trans-cisternae and that specialized ER plays a significant role in trans-Golgi function.

Bibliography

Ladinsky, M. S., Wu, C. C., McIntosh, S., McIntosh, J. R., & Howell, K. E. (2002). Structure of the Golgi and Distribution of Reporter Molecules at 20Â°C Reveals the Complexity of the Exit Compartments. Molecular Biology of the Cell, 13(8), 2810â2825.

Authors 5

Mark S. Ladinsky (first)
Christine C. Wu (additional)
Shane McIntosh (additional)
J. Richard McIntosh (additional)
Kathryn E. Howell (additional)

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Dates

Type	When
Created	22 years, 10 months ago (Sept. 30, 2002, 1:42 p.m.)
Deposited	4 years, 2 months ago (May 28, 2021, 4:51 a.m.)
Indexed	1 year ago (Aug. 9, 2024, 1:56 a.m.)
Issued	23 years ago (Aug. 1, 2002)
Published	23 years ago (Aug. 1, 2002)
Published Print	23 years ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Ladinsky_2002, title={Structure of the Golgi and Distribution of Reporter Molecules at 20°C Reveals the Complexity of the Exit Compartments}, volume={13}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.01-12-0593}, DOI={10.1091/mbc.01-12-0593}, number={8}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Ladinsky, Mark S. and Wu, Christine C. and McIntosh, Shane and McIntosh, J. Richard and Howell, Kathryn E.}, editor={Bonifacino, Juan}, year={2002}, month=aug, pages={2810–2825} }

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