DOI: 10.1091/mbc.01-07-0348. Function of Dynein and Dynactin in Herpes Simplex Virus Capsid Transport

Function of Dynein and Dynactin in Herpes Simplex Virus Capsid Transport

10.1091/mbc.01-07-0348

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate most transport processes directed toward the minus-ends of MTs. Immunofluorescence microscopy experiments demonstrated that HSV1 capsids colocalized with cytoplasmic dynein and dynactin. We blocked the function of dynein by overexpressing the dynactin subunit dynamitin, which leads to the disruption of the dynactin complex. We then infected such cells with HSV1 and measured the efficiency of particle binding, virus entry, capsid transport to the nucleus, and the expression of immediate-early viral genes. High concentrations of dynamitin and dynamitin-GFP reduced the number of viral capsids transported to the nucleus. Moreover, viral protein synthesis was inhibited, whereas virus binding to the plasma membrane, its internalization, and the organization of the MT network were not affected. We concluded that incoming HSV1 capsids are propelled along MTs by dynein and that dynein and dynactin are required for efficient viral capsid transport to the nucleus.

Bibliography

DÃ¶hner, K., Wolfstein, A., Prank, U., Echeverri, C., Dujardin, D., Vallee, R., & Sodeik, B. (2002). Function of Dynein and Dynactin in Herpes Simplex Virus Capsid Transport. Molecular Biology of the Cell, 13(8), 2795â2809.

Authors 7

Katinka Döhner (first)
André Wolfstein (additional)
Ute Prank (additional)
Christophe Echeverri (additional)
Denis Dujardin (additional)
Richard Vallee (additional)
Beate Sodeik (additional)

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Dates

Type	When
Created	22 years, 11 months ago (Sept. 30, 2002, 1:42 p.m.)
Deposited	4 years, 3 months ago (May 28, 2021, 4:51 a.m.)
Indexed	3 months, 3 weeks ago (May 12, 2025, 11:03 a.m.)
Issued	23 years, 1 month ago (Aug. 1, 2002)
Published	23 years, 1 month ago (Aug. 1, 2002)
Published Print	23 years, 1 month ago (Aug. 1, 2002)

Funders 0

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BibTeX

@article{D_hner_2002, title={Function of Dynein and Dynactin in Herpes Simplex Virus Capsid Transport}, volume={13}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.01-07-0348}, DOI={10.1091/mbc.01-07-0348}, number={8}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Döhner, Katinka and Wolfstein, André and Prank, Ute and Echeverri, Christophe and Dujardin, Denis and Vallee, Richard and Sodeik, Beate}, editor={Goldstein, Lawrence S.}, year={2002}, month=aug, pages={2795–2809} }

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