DOI: 10.1091/mbc.01-05-0264. Mutation of<i>YCS4</i>, a Budding Yeast Condensin Subunit, Affects Mitotic and Nonmitotic Chromosome Behavior

Mutation ofYCS4, a Budding Yeast Condensin Subunit, Affects Mitotic and Nonmitotic Chromosome Behavior

10.1091/mbc.01-05-0264

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The budding yeast YCS4 gene encodes a conserved regulatory subunit of the condensin complex. We isolated an allele of this gene in a screen for mutants defective in sister chromatid separation or segregation. The phenotype of the ycs4-1mutant is similar to topoisomerase II mutants and distinct from theesp1-1 mutant: the topological resolution of sister chromatids is compromised in ycs4-1 despite normal removal of cohesins from mitotic chromosomes. Consistent with a role in sister separation, YCS4 function is required to localize DNA topoisomerase I and II to chromosomes. Unlike its homologs inXenopus and fission yeast, Ycs4p is associated with chromatin throughout the cell cycle; the only change in localization occurs during anaphase when the protein is enriched at the nucleolus. This relocalization may reveal the specific challenge that segregation of the transcriptionally hyperactive, repetitive array of rDNA genes can present during mitosis. Indeed, segregation of the nucleolus is abnormal in ycs4-1 at the nonpermissive temperature. Interrepeat recombination in the rDNA array is specifically elevated inycs4-1 at the permissive temperature, suggesting that the Ycs4p plays a role at the array aside from its segregation. Furthermore, ycs4-1 is defective in silencing at the mating type loci at the permissive temperature. Taken together, our data suggest that there are mitotic as well as nonmitotic chromosomal abnormalities associated with loss of condensin function in budding yeast.

Bibliography

Bhalla, N., Biggins, S., & Murray, A. W. (2002). Mutation ofYCS4, a Budding Yeast Condensin Subunit, Affects Mitotic and Nonmitotic Chromosome Behavior. Molecular Biology of the Cell, 13(2), 632â645.

Authors 3

Needhi Bhalla (first)
Sue Biggins (additional)
Andrew W. Murray (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 6:19 p.m.)
Deposited	4 years, 3 months ago (May 17, 2021, 11:36 a.m.)
Indexed	1 month, 1 week ago (July 12, 2025, 6:47 p.m.)
Issued	23 years, 6 months ago (Feb. 1, 2002)
Published	23 years, 6 months ago (Feb. 1, 2002)
Published Print	23 years, 6 months ago (Feb. 1, 2002)

Funders 0

None

BibTeX

@article{Bhalla_2002, title={Mutation ofYCS4, a Budding Yeast Condensin Subunit, Affects Mitotic and Nonmitotic Chromosome Behavior}, volume={13}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.01-05-0264}, DOI={10.1091/mbc.01-05-0264}, number={2}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Bhalla, Needhi and Biggins, Sue and Murray, Andrew W.}, editor={Yanagida, Mitsuhiro}, year={2002}, month=feb, pages={632–645} }

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  "abstract": "<jats:p>The budding yeast YCS4 gene encodes a conserved regulatory subunit of the condensin complex. We isolated an allele of this gene in a screen for mutants defective in sister chromatid separation or segregation. The phenotype of the ycs4-1mutant is similar to topoisomerase II mutants and distinct from theesp1-1 mutant: the topological resolution of sister chromatids is compromised in ycs4-1 despite normal removal of cohesins from mitotic chromosomes. Consistent with a role in sister separation, YCS4 function is required to localize DNA topoisomerase I and II to chromosomes. Unlike its homologs inXenopus and fission yeast, Ycs4p is associated with chromatin throughout the cell cycle; the only change in localization occurs during anaphase when the protein is enriched at the nucleolus. This relocalization may reveal the specific challenge that segregation of the transcriptionally hyperactive, repetitive array of rDNA genes can present during mitosis. Indeed, segregation of the nucleolus is abnormal in ycs4-1 at the nonpermissive temperature. Interrepeat recombination in the rDNA array is specifically elevated inycs4-1 at the permissive temperature, suggesting that the Ycs4p plays a role at the array aside from its segregation. Furthermore, ycs4-1 is defective in silencing at the mating type loci at the permissive temperature. Taken together, our data suggest that there are mitotic as well as nonmitotic chromosomal abnormalities associated with loss of condensin function in budding yeast.</jats:p>",
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