DOI: 10.1085/jgp.45.4.703. Photoenzymatic Repair of Ultraviolet Damage in DNA

Photoenzymatic Repair of Ultraviolet Damage in DNA

10.1085/jgp.45.4.703

Crossref journal-article

Rockefeller University Press

The Journal of General Physiology (291)

Abstract

As previously reported, ultraviolet-inactivated bacterial transforming DNA can be restored to activity by an enzyme-like agent from bakers' yeast which requires light for its activity. Kinetics of this reaction, in the presence and absence of inhibitors, are found consistent with the Michaelis-Menten reaction scheme, with the sites of ultraviolet damage on the DNA serving as substrate and the repaired structure as product. Kinetic studies with different light intensities suggest that the necessary illumination causes photolysis of the enzyme-substrate complex with concurrent repair of the DNA. Competitive inhibition of irradiated transforming DNA repair, which occurs when irradiated non-transforming DNA is present in the same reaction mixture, permits ultraviolet damage (of the kind capable of being photoreactivated) to be detected in any type of DNA.

Bibliography

Rupert, C. S. (1962). Photoenzymatic Repair of Ultraviolet Damage in DNA. The Journal of General Physiology, 45(4), 703â724.

Authors 1

Claud S. Rupert (first)

References 0 Referenced 104

None

Dates

Type	When
Created	21 years, 3 months ago (May 13, 2004, 6:11 p.m.)
Deposited	2 years, 1 month ago (July 27, 2023, 3:56 a.m.)
Indexed	1 year, 9 months ago (Dec. 5, 2023, 12:24 p.m.)
Issued	63 years, 6 months ago (March 1, 1962)
Published	63 years, 6 months ago (March 1, 1962)
Published Online	63 years, 6 months ago (March 1, 1962)
Published Print	63 years, 6 months ago (March 1, 1962)

Funders 0

None

BibTeX

@article{Rupert_1962, title={Photoenzymatic Repair of Ultraviolet Damage in DNA}, volume={45}, ISSN={0022-1295}, url={http://dx.doi.org/10.1085/jgp.45.4.703}, DOI={10.1085/jgp.45.4.703}, number={4}, journal={The Journal of General Physiology}, publisher={Rockefeller University Press}, author={Rupert, Claud S.}, year={1962}, month=mar, pages={703–724} }

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