DOI: 10.1085/jgp.36.2.173. UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI

UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI

10.1085/jgp.36.2.173

Crossref journal-article

Rockefeller University Press

Journal of General Physiology (291)

Abstract

1. The uptake of glycine-N15 by components of cell nuclei was studied. The nuclear components were derived both from tissues with high metabolic rates-mammalian liver, kidney, and pancreas-and from cells with relatively low rates of metabolism-avian erythrocytes and echinoderm sperm. N15 uptake by nuclear components of liver, kidney, and pancreas was far more rapid than by those of erythrocytes and sperm. 2. The nuclear components of liver, kidney, and pancreas for which measurements were made were DNA, histone, and residual protein of chromatin. Uptake into DNA was low, into histone higher, and into residual protein much higher still, being comparable with that into mixed cytoplasmic protein. 3. A comparison of the uptake of N15 by the chromosomal components, histone and DNA of liver, pancreas, and kidney showed that chromosomal "activity" varies in different cells and also in the same cell depending upon its over-all activity.

Bibliography

Daly, M. M., Allfrey, V. G., & Mirsky, A. E. (1952). UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI. Journal of General Physiology, 36(2), 173â179.

Authors 3

Marie M. Daly (first)
V. G. Allfrey (additional)
A. E. Mirsky (additional)

References 0 Referenced 78

None

Dates

Type	When
Created	21 years, 3 months ago (May 13, 2004, 5:41 p.m.)
Deposited	4 years, 10 months ago (Oct. 3, 2020, 3:31 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 3:57 p.m.)
Issued	72 years, 9 months ago (Nov. 20, 1952)
Published	72 years, 9 months ago (Nov. 20, 1952)
Published Online	72 years, 9 months ago (Nov. 20, 1952)
Published Print	72 years, 9 months ago (Nov. 20, 1952)

Funders 0

None

BibTeX

@article{Daly_1952, title={UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI}, volume={36}, ISSN={0022-1295}, url={http://dx.doi.org/10.1085/jgp.36.2.173}, DOI={10.1085/jgp.36.2.173}, number={2}, journal={Journal of General Physiology}, publisher={Rockefeller University Press}, author={Daly, Marie M. and Allfrey, V. G. and Mirsky, A. E.}, year={1952}, month=nov, pages={173–179} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T20:57:42Z",
    "timestamp": 1705006662384
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "2",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1952,
        11,
        20
      ]
    ]
  },
  "abstract": "<jats:p>1. The uptake of glycine-N15 by components of cell nuclei was studied. The nuclear components were derived both from tissues with high metabolic rates-mammalian liver, kidney, and pancreas-and from cells with relatively low rates of metabolism-avian erythrocytes and echinoderm sperm. N15 uptake by nuclear components of liver, kidney, and pancreas was far more rapid than by those of erythrocytes and sperm.</jats:p>\n               <jats:p>2. The nuclear components of liver, kidney, and pancreas for which measurements were made were DNA, histone, and residual protein of chromatin. Uptake into DNA was low, into histone higher, and into residual protein much higher still, being comparable with that into mixed cytoplasmic protein.</jats:p>\n               <jats:p>3. A comparison of the uptake of N15 by the chromosomal components, histone and DNA of liver, pancreas, and kidney showed that chromosomal \"activity\" varies in different cells and also in the same cell depending upon its over-all activity.</jats:p>",
  "DOI": "10.1085/jgp.36.2.173",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        5,
        13
      ]
    ],
    "date-time": "2004-05-13T21:41:10Z",
    "timestamp": 1084484470000
  },
  "page": "173-179",
  "update-policy": "http://dx.doi.org/10.1085/jgp.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 78,
  "title": "UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI",
  "prefix": "10.1085",
  "volume": "36",
  "author": [
    {
      "given": "Marie M.",
      "family": "Daly",
      "sequence": "first",
      "affiliation": [
        {
          "name": "From the Laboratories of The Rockefeller Institute for Medical Research"
        }
      ]
    },
    {
      "given": "V. G.",
      "family": "Allfrey",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratories of The Rockefeller Institute for Medical Research"
        }
      ]
    },
    {
      "given": "A. E.",
      "family": "Mirsky",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratories of The Rockefeller Institute for Medical Research"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1952,
        11,
        20
      ]
    ]
  },
  "container-title": "Journal of General Physiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://rupress.org/jgp/article-pdf/36/2/173/1240803/173.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://rupress.org/jgp/article-pdf/36/2/173/1240803/173.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2020,
        10,
        3
      ]
    ],
    "date-time": "2020-10-03T19:31:14Z",
    "timestamp": 1601753474000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jgp/article/36/2/173/47444/UPTAKE-OF-GLYCINEN15-BY-COMPONENTS-OF-CELL-NUCLEI"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1952,
        11,
        20
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1952,
          11,
          20
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1085/jgp.36.2.173",
  "relation": {},
  "ISSN": [
    "1540-7748",
    "0022-1295"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1952,
        11,
        20
      ]
    ]
  }
}