DOI: 10.1085/jgp.117.2.165. Molecular Architecture of Full-Length <i>KcsA</i>

Molecular Architecture of Full-Length KcsA

10.1085/jgp.117.2.165

Crossref journal-article

Rockefeller University Press

The Journal of General Physiology (291)

Abstract

The molecular architecture of the NH2 and COOH termini of the prokaryotic potassium channel KcsA has been determined using site-directed spin-labeling methods and paramagnetic resonance EPR spectroscopy. Cysteine mutants were generated (residues 5–24 and 121–160) and spin labeled, and the X-band CW EPR spectra were obtained from liposome-reconstituted channels at room temperature. Data on probe mobility (ΔHo−1), accessibility parameters (ΠO2 and ΠNiEdda), and inter-subunit spin-spin interaction (Ω) were used as structural constraints to build a three-dimensional folding model of these cytoplasmic domains from a set of simulated annealing and restrained molecular dynamics runs. 32 backbone structures were generated and averaged using fourfold symmetry, and a final mean structure was obtained from the eight lowest energy runs. Based on the present data, together with information from the KcsA crystal structure, a model for the three-dimensional fold of full-length KcsA was constructed. In this model, the NH2 terminus of KcsA forms an α-helix anchored at the membrane–water interface, while the COOH terminus forms a right-handed four-helix bundle that extend some 40–50 Å towards the cytoplasm. Functional analysis of COOH-terminal deletion constructs suggest that, while the COOH terminus does not play a substantial role in determining ion permeation properties, it exerts a modulatory role in the pH-dependent gating mechanism.

Bibliography

Cortes, D. M., Cuello, L. G., & Perozo, E. (2001). Molecular Architecture of Full-Length KcsA. The Journal of General Physiology, 117(2), 165â180.

Authors 3

D. Marien Cortes (first)
Luis G. Cuello (additional)
Eduardo Perozo (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:50 p.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 3:34 p.m.)
Indexed	1 month, 2 weeks ago (July 23, 2025, 8:58 a.m.)
Issued	24 years, 7 months ago (Jan. 29, 2001)
Published	24 years, 7 months ago (Jan. 29, 2001)
Published Online	24 years, 7 months ago (Jan. 29, 2001)
Published Print	24 years, 7 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Cortes_2001, title={Molecular Architecture of Full-Length KcsA}, volume={117}, ISSN={1540-7748}, url={http://dx.doi.org/10.1085/jgp.117.2.165}, DOI={10.1085/jgp.117.2.165}, number={2}, journal={The Journal of General Physiology}, publisher={Rockefeller University Press}, author={Cortes, D. Marien and Cuello, Luis G. and Perozo, Eduardo}, year={2001}, month=jan, pages={165–180} }

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  "abstract": "<jats:p>The molecular architecture of the NH2 and COOH termini of the prokaryotic potassium channel KcsA has been determined using site-directed spin-labeling methods and paramagnetic resonance EPR spectroscopy. Cysteine mutants were generated (residues 5\u201324 and 121\u2013160) and spin labeled, and the X-band CW EPR spectra were obtained from liposome-reconstituted channels at room temperature. Data on probe mobility (\u0394Ho\u22121), accessibility parameters (\u03a0O2 and \u03a0NiEdda), and inter-subunit spin-spin interaction (\u03a9) were used as structural constraints to build a three-dimensional folding model of these cytoplasmic domains from a set of simulated annealing and restrained molecular dynamics runs. 32 backbone structures were generated and averaged using fourfold symmetry, and a final mean structure was obtained from the eight lowest energy runs. Based on the present data, together with information from the KcsA crystal structure, a model for the three-dimensional fold of full-length KcsA was constructed. In this model, the NH2 terminus of KcsA forms an \u03b1-helix anchored at the membrane\u2013water interface, while the COOH terminus forms a right-handed four-helix bundle that extend some 40\u201350 \u00c5 towards the cytoplasm. Functional analysis of COOH-terminal deletion constructs suggest that, while the COOH terminus does not play a substantial role in determining ion permeation properties, it exerts a modulatory role in the pH-dependent gating mechanism.</jats:p>",
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