DOI: 10.1085/jgp.110.3.283. Plasma Membrane Water Permeability of Cultured Cells and Epithelia Measured by Light Microscopy with Spatial Filtering

Plasma Membrane Water Permeability of Cultured Cells and Epithelia Measured by Light Microscopy with Spatial Filtering

10.1085/jgp.110.3.283

Crossref journal-article

Rockefeller University Press

The Journal of General Physiology (291)

Abstract

A method was developed to measure the osmotic water permeability (Pf) of plasma membranes in cell layers and applied to cells and epithelia expressing molecular water channels. It was found that the integrated intensity of monochromatic light in a phase contrast or dark field microscope was dependent on relative cell volume. For cells of different size and shape (Sf9, MDCK, CHO, A549, tracheal epithelia, BHK), increased cell volume was associated with decreased signal intensity; generally the signal decreased 10–20% for a twofold increase in cell volume. A theory relating signal intensity to relative cell volume was developed based on spatial filtering and changes in optical path length associated with cell volume changes. Theory predictions were confirmed by signal measurements of cell layers bathed in solutions of various osmolarities and refractive indices. The excellent signal-to-noise ratio of the transmitted light detection permitted measurement of cell volume changes of &lt;1%. The method was applied to characterize transfected cells and tissues that natively express water channels. Pf in control Chinese hamster ovary cells was low (0.0012 cm/s at 23°C) and increased more than fourfold upon stable transfection with aquaporins 1, 2, 4, or 5. Pf in apical and basolateral membranes in polarized epithelial cells grown on porous supports was measured. Pfbl and Pfap were 0.0011 and 0.0024 cm/s (MDCK cells), and 0.0039 and 0.0052 cm/s (human tracheal cells) at 23°C. In intact toad urinary bladder, basolateral Pf was 0.036 cm/s and apical membrane Pf after vasopressin stimulation was 0.025 cm/s at 23°C. The results establish light microscopy with spatial filtering as a technically simple and quantitative method to measure water permeability in cell layers and provide the first measurement of the apical and basolateral membrane permeabilities of several important epithelial cell types.

Bibliography

Farinas, J., Kneen, M., Moore, M., & Verkman, A. S. (1997). Plasma Membrane Water Permeability of Cultured Cells and Epithelia Measured by Light Microscopy with Spatial Filtering. The Journal of General Physiology, 110(3), 283â296.

Authors 4

Javier Farinas (first)
Malea Kneen (additional)
Megan Moore (additional)
A.S. Verkman (additional)

References 37 Referenced 93

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10.1007/BF00371117 / Pflugers Arch / A simple method for monitoring changes in cell height using fluorescent microbeads and an Ussing-type chamber for the inverted microscope by Crowe (1991)
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10.1016/S0006-3495(95)80335-8 / Biophys J / Cell volume measured in adherent cells by total internal reflection microfluorimetry: application to permeability in cells transfected with water channel homologs by Farinas (1995)
10.1016/S0006-3495(96)79546-2 / Biophys J / Cell volume and plasma membrane osmotic water permeability in epithelial cell layers measured by interferometry by Farinas (1996)
10.1152/ajpcell.1993.265.5.C1412 / Am J Physiol / Determination of volume and water permeability of plated cells from measurements of light scattering by Fischbarg (1993)
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10.1073/pnas.92.10.4328 / Proc Natl Acad Sci USA / Immunolocalization of the mercurial-insensitive water channel and glycerol intrinsic protein in epithelial cell plasma membranes by Frigeri (1995)
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{'key': '2023072520202699600_B12'}
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{'key': '2023072520202699600_B14'}
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10.1016/S0021-9258(18)41591-8 / J Biol Chem / Localization and functional analysis of CHIP28k water channels in stably transfected CHO cells by Ma (1993)
{'key': '2023072520202699600_B21'}
10.1681/ASN.V13278 / J Am Soc Nephrol / Transepithelial water permeability in an in vitro model of renal cysts by Mangoo-Karim (1990)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:50 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 4:21 p.m.)
Indexed	2 months ago (July 1, 2025, 6:04 a.m.)
Issued	28 years ago (Sept. 1, 1997)
Published	28 years ago (Sept. 1, 1997)
Published Online	28 years ago (Sept. 1, 1997)
Published Print	28 years ago (Sept. 1, 1997)

Funders 0

None

BibTeX

@article{Farinas_1997, title={Plasma Membrane Water Permeability of Cultured Cells and Epithelia Measured by Light Microscopy with Spatial Filtering}, volume={110}, ISSN={1540-7748}, url={http://dx.doi.org/10.1085/jgp.110.3.283}, DOI={10.1085/jgp.110.3.283}, number={3}, journal={The Journal of General Physiology}, publisher={Rockefeller University Press}, author={Farinas, Javier and Kneen, Malea and Moore, Megan and Verkman, A.S.}, year={1997}, month=sep, pages={283–296} }

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