DOI: 10.1084/jem.99.2.167. PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

10.1084/jem.99.2.167

Crossref journal-article

Rockefeller University Press

Journal of Experimental Medicine (291)

Abstract

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 108 plaque-forming particles per ml., were obtained.

Bibliography

Dulbecco, R., & Vogt, M. (1954). PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES. Journal of Experimental Medicine, 99(2), 167â182.

Authors 2

R. Dulbecco (first)
Marguerite Vogt (additional)

References 0 Referenced 2,843

None

Dates

Type	When
Created	21 years, 2 months ago (June 22, 2004, 2:35 p.m.)
Deposited	4 years, 10 months ago (Oct. 3, 2020, 10:18 a.m.)
Indexed	4 days, 20 hours ago (Aug. 22, 2025, 12:46 a.m.)
Issued	71 years, 6 months ago (Feb. 1, 1954)
Published	71 years, 6 months ago (Feb. 1, 1954)
Published Online	71 years, 6 months ago (Feb. 1, 1954)
Published Print	71 years, 6 months ago (Feb. 1, 1954)

Funders 0

None

BibTeX

@article{Dulbecco_1954, title={PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES}, volume={99}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.99.2.167}, DOI={10.1084/jem.99.2.167}, number={2}, journal={Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Dulbecco, R. and Vogt, Marguerite}, year={1954}, month=feb, pages={167–182} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        22
      ]
    ],
    "date-time": "2025-08-22T04:46:15Z",
    "timestamp": 1755837975186
  },
  "reference-count": 0,
  "publisher": "Rockefeller University Press",
  "issue": "2",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1954,
        2,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum.</jats:p>\n               <jats:p>Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain.</jats:p>\n               <jats:p>High titer virus stocks, with titers up to 7 x 108 plaque-forming particles per ml., were obtained.</jats:p>",
  "DOI": "10.1084/jem.99.2.167",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        6,
        22
      ]
    ],
    "date-time": "2004-06-22T18:35:50Z",
    "timestamp": 1087929350000
  },
  "page": "167-182",
  "update-policy": "http://dx.doi.org/10.1084/jem.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 2843,
  "title": "PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES",
  "prefix": "10.1084",
  "volume": "99",
  "author": [
    {
      "given": "R.",
      "family": "Dulbecco",
      "sequence": "first",
      "affiliation": [
        {
          "name": "From the California Institute of Technology, Pasadena"
        }
      ]
    },
    {
      "given": "Marguerite",
      "family": "Vogt",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the California Institute of Technology, Pasadena"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1954,
        2,
        1
      ]
    ]
  },
  "container-title": "Journal of Experimental Medicine",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://rupress.org/jem/article-pdf/99/2/167/1185313/167.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://rupress.org/jem/article-pdf/99/2/167/1185313/167.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2020,
        10,
        3
      ]
    ],
    "date-time": "2020-10-03T14:18:06Z",
    "timestamp": 1601734686000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jem/article/99/2/167/10779/PLAQUE-FORMATION-AND-ISOLATION-OF-PURE-LINES-WITH"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1954,
        2,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1954,
          2,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1084/jem.99.2.167",
  "relation": {},
  "ISSN": [
    "1540-9538",
    "0022-1007"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1954,
        2,
        1
      ]
    ]
  }
}