DOI: 10.1084/jem.20051027. Interaction of CagA with Crk plays an important role in <i>Helicobacter pylori</i>–induced loss of gastric epithelial cell adhesion

Interaction of CagA with Crk plays an important role in Helicobacter pylori–induced loss of gastric epithelial cell adhesion

10.1084/jem.20051027

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

CagA protein is a major virulence factor of Helicobacter pylori, which is delivered into gastric epithelial cells and elicits growth factor–like responses. Once within the cells, CagA is tyrosine phosphorylated by Src family kinases and targets host proteins required to induce the cell responses. We show that the phosphorylated CagA binds Crk adaptor proteins (Crk-II, Crk-I, and Crk-L) and that the interaction is important for the CagA-mediated host responses during H. pylori infection. H. pylori–induced scattering of gastric epithelial cells in culture was blocked by overexpression of dominant-negative Crk and by RNA interference–mediated knockdown of endogenous Crk. H. pylori infection of the gastric epithelium induced disruption of E-cadherin/catenin–containing adherens junctions, which was also dependent on CagA/Crk signaling. Furthermore, inhibition of the SoS1/H-Ras/Raf1, C3G/Rap1/B-Raf, or Dock180/Rac1/Wiskott-Aldrich syndrome protein family verprolin homologous protein pathway, all of which are involved downstream of Crk adaptors, greatly diminished the CagA-associated host responses. Thus, CagA targeting of Crk plays a central role in inducing the pleiotropic cell responses to H. pylori infection that cause several gastric diseases, including gastric cancer.

Bibliography

Suzuki, M., Mimuro, H., Suzuki, T., Park, M., Yamamoto, T., & Sasakawa, C. (2005). Interaction of CagA with Crk plays an important role in Helicobacter pyloriâinduced loss of gastric epithelial cell adhesion. The Journal of Experimental Medicine, 202(9), 1235â1247.

Authors 6

Masato Suzuki (first)
Hitomi Mimuro (additional)
Toshihiko Suzuki (additional)
Morag Park (additional)
Tadashi Yamamoto (additional)
Chihiro Sasakawa (additional)

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Dates

Type	When
Created	19 years, 9 months ago (Nov. 7, 2005, 3:03 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 9:33 a.m.)
Indexed	4 months, 1 week ago (April 28, 2025, 7:27 p.m.)
Issued	19 years, 9 months ago (Nov. 7, 2005)
Published	19 years, 9 months ago (Nov. 7, 2005)
Published Online	19 years, 9 months ago (Nov. 7, 2005)
Published Print	19 years, 9 months ago (Nov. 7, 2005)

Funders 0

None

BibTeX

@article{Suzuki_2005, title={Interaction of CagA with Crk plays an important role in Helicobacter pylori–induced loss of gastric epithelial cell adhesion}, volume={202}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20051027}, DOI={10.1084/jem.20051027}, number={9}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Suzuki, Masato and Mimuro, Hitomi and Suzuki, Toshihiko and Park, Morag and Yamamoto, Tadashi and Sasakawa, Chihiro}, year={2005}, month=nov, pages={1235–1247} }

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