DOI: 10.1084/jem.20041930. Type I interferon dependence of plasmacytoid dendritic cell activation and migration

Type I interferon dependence of plasmacytoid dendritic cell activation and migration

10.1084/jem.20041930

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Differential expression of Toll-like receptor (TLR) by conventional dendritic cells (cDCs) and plasmacytoid DC (pDCs) has been suggested to influence the type of immune response induced by microbial pathogens. In this study we show that, in vivo, cDCs and pDCs are equally activated by TLR4, -7, and -9 ligands. Type I interferon (IFN) was important for pDC activation in vivo in response to all three TLR ligands, whereas cDCs required type I IFN signaling only for TLR9- and partially for TLR7-mediated activation. Although TLR ligands induced in situ migration of spleen cDC into the T cell area, spleen pDCs formed clusters in the marginal zone and in the outer T cell area 6 h after injection of TLR9 and TLR7 ligands, respectively. In vivo treatment with TLR9 ligands decreased pDC ability to migrate ex vivo in response to IFN-induced CXCR3 ligands and increased their response to CCR7 ligands. Unlike cDCs, the migration pattern of pDCs required type I IFN for induction of CXCR3 ligands and responsiveness to CCR7 ligands. These data demonstrate that mouse pDCs differ from cDCs in the in vivo response to TLR ligands, in terms of pattern and type I IFN requirement for activation and migration.

Bibliography

Asselin-Paturel, C., Brizard, G., Chemin, K., Boonstra, A., OâGarra, A., Vicari, A., & Trinchieri, G. (2005). Type I interferon dependence of plasmacytoid dendritic cell activation and migration. The Journal of Experimental Medicine, 201(7), 1157â1167.

Authors 7

Carine Asselin-Paturel (first)
Géraldine Brizard (additional)
Karine Chemin (additional)
Andre Boonstra (additional)
Anne O'Garra (additional)
Alain Vicari (additional)
Giorgio Trinchieri (additional)

References 43 Referenced 275

10.1038/32588 / Nature. (1998)
10.1038/35100529 / Nat. Rev. Immunol. (2001)
10.1016/S0092-8674(00)80059-8 / Cell. (1999)
10.1034/j.1600-065X.2000.17717.x / Immunol. Rev. (2000)
10.1038/nri746 / Nat. Rev. Immunol. (2002)
{'key': '2023072513044800200_BIB6', 'first-page': '120', 'volume': '4', 'year': '1985', 'journal-title': 'Nat. Immun. Cell Growth Regul.'} / Nat. Immun. Cell Growth Regul. (1985)
10.1126/science.284.5421.1835 / Science. (1999)
10.4049/jimmunol.166.4.2291 / J. Immunol. (2001)
10.1038/ni736 / Nat. Immunol. (2001)
10.1084/jem.194.8.1171 / J. Exp. Med. (2001)
10.1182/blood.V98.13.3520 / Blood. (2001)
10.1084/jem.191.3.411 / J. Exp. Med. (2000)
10.1084/jem.20021908 / J. Exp. Med. (2003)
10.1126/science.283.5405.1183 / Science. (1999)
10.1016/S0092-8674(01)00456-1 / Cell. (2001)
10.1002/jlb.66.2.224 / J. Leukoc. Biol. (1999)
10.1084/jem.20012040 / J. Exp. Med. (2002)
10.1084/jem.164.1.180 / J. Exp. Med. (1986)
10.1002/eji.200323797 / Eur. J. Immunol. (2003)
10.1016/j.tim.2004.06.001 / Trends Microbiol. (2004)
10.1016/j.virol.2004.01.033 / Virology. (2004)
10.1073/pnas.0400937101 / Proc. Natl. Acad. Sci. USA. (2004)
10.1084/jem.20030162 / J. Exp. Med. (2003)
{'key': '2023072513044800200_BIB24', 'first-page': '2489', 'volume': '58', 'year': '1998', 'journal-title': 'Cancer Res.'} / Cancer Res. (1998)
10.1111/j.1600-065X.1995.tb00090.x / Immunol. Rev. (1995)
10.1016/S1074-7613(01)00154-6 / Immunity. (2001)
10.1073/pnas.1934678100 / Proc. Natl. Acad. Sci. USA. (2003)
10.1093/intimm/dxf089 / Int. Immunol. (2002)
10.1128/IAI.72.2.824-832.2004 / Infect. Immun. (2004)
10.1084/jem.186.11.1819 / J. Exp. Med. (1997)
10.4049/jimmunol.171.12.6466 / J. Immunol. (2003)
10.4049/jimmunol.169.11.6079 / J. Immunol. (2002)
10.1016/S0198-8859(02)00755-3 / Hum. Immunol. (2002)
10.1002/1521-4141(200111)31:11<3388::AID-IMMU3388>3.0.CO;2-Q / Eur. J. Immunol. (2001)
10.1182/blood.V99.9.3263 / Blood. (2002)
10.4049/jimmunol.161.4.1947 / J. Immunol. (1998)
10.1111/j.1600-065X.1997.tb00956.x / Immunol. Rev. (1997)
10.1046/j.1365-3083.1999.00514.x / Scand. J. Immunol. (1999)
10.4049/jimmunol.167.4.1862 / J. Immunol. (2001)
10.1084/jem.20020437 / J. Exp. Med. (2003)
10.1093/intimm/dxh093 / Int. Immunol. (2004)
10.1189/jlb.0603291 / J. Leukoc. Biol. (2004)
10.1189/jlb.71.4.669 / J. Leukoc. Biol. (2002)

Dates

Type	When
Created	20 years, 5 months ago (March 28, 2005, 8:12 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 9:09 a.m.)
Indexed	1 month ago (July 24, 2025, 8:09 a.m.)
Issued	20 years, 5 months ago (March 28, 2005)
Published	20 years, 5 months ago (March 28, 2005)
Published Online	20 years, 5 months ago (March 28, 2005)
Published Print	20 years, 4 months ago (April 4, 2005)

Funders 0

None

BibTeX

@article{Asselin_Paturel_2005, title={Type I interferon dependence of plasmacytoid dendritic cell activation and migration}, volume={201}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20041930}, DOI={10.1084/jem.20041930}, number={7}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Asselin-Paturel, Carine and Brizard, Géraldine and Chemin, Karine and Boonstra, Andre and O’Garra, Anne and Vicari, Alain and Trinchieri, Giorgio}, year={2005}, month=mar, pages={1157–1167} }

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      "first-page": "134",
      "DOI": "10.1034/j.1600-065X.2000.17717.x",
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    },
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      "first-page": "151",
      "DOI": "10.1038/nri746",
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    },
    {
      "key": "2023072513044800200_BIB6",
      "first-page": "120",
      "volume": "4",
      "year": "1985",
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    },
    {
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      "first-page": "1835",
      "DOI": "10.1126/science.284.5421.1835",
      "volume": "284",
      "year": "1999",
      "journal-title": "Science."
    },
    {
      "key": "2023072513044800200_BIB8",
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      "first-page": "2291",
      "DOI": "10.4049/jimmunol.166.4.2291",
      "volume": "166",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072513044800200_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "1144",
      "DOI": "10.1038/ni736",
      "volume": "2",
      "year": "2001",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "2023072513044800200_BIB10",
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      "first-page": "1171",
      "DOI": "10.1084/jem.194.8.1171",
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      "DOI": "10.1182/blood.V98.13.3520",
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      "journal-title": "Blood."
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      "first-page": "411",
      "DOI": "10.1084/jem.191.3.411",
      "volume": "191",
      "year": "2000",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072513044800200_BIB13",
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      "first-page": "101",
      "DOI": "10.1084/jem.20021908",
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      "year": "2003",
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      "key": "2023072513044800200_BIB15",
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      "DOI": "10.1016/S0092-8674(01)00456-1",
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      "journal-title": "Cell."
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    {
      "key": "2023072513044800200_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "224",
      "DOI": "10.1002/jlb.66.2.224",
      "volume": "66",
      "year": "1999",
      "journal-title": "J. Leukoc. Biol."
    },
    {
      "key": "2023072513044800200_BIB17",
      "doi-asserted-by": "crossref",
      "first-page": "F9",
      "DOI": "10.1084/jem.20012040",
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      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072513044800200_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "180",
      "DOI": "10.1084/jem.164.1.180",
      "volume": "164",
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      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072513044800200_BIB19",
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      "first-page": "827",
      "DOI": "10.1002/eji.200323797",
      "volume": "33",
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      "key": "2023072513044800200_BIB20",
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      "DOI": "10.1016/j.tim.2004.06.001",
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      "DOI": "10.1073/pnas.0400937101",
      "volume": "101",
      "year": "2004",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072513044800200_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "513",
      "DOI": "10.1084/jem.20030162",
      "volume": "198",
      "year": "2003",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072513044800200_BIB24",
      "first-page": "2489",
      "volume": "58",
      "year": "1998",
      "journal-title": "Cancer Res."
    },
    {
      "key": "2023072513044800200_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "5",
      "DOI": "10.1111/j.1600-065X.1995.tb00090.x",
      "volume": "148",
      "year": "1995",
      "journal-title": "Immunol. Rev."
    },
    {
      "key": "2023072513044800200_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "661",
      "DOI": "10.1016/S1074-7613(01)00154-6",
      "volume": "14",
      "year": "2001",
      "journal-title": "Immunity."
    },
    {
      "key": "2023072513044800200_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "10872",
      "DOI": "10.1073/pnas.1934678100",
      "volume": "100",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072513044800200_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "1225",
      "DOI": "10.1093/intimm/dxf089",
      "volume": "14",
      "year": "2002",
      "journal-title": "Int. Immunol."
    },
    {
      "key": "2023072513044800200_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "824",
      "DOI": "10.1128/IAI.72.2.824-832.2004",
      "volume": "72",
      "year": "2004",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "2023072513044800200_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "1819",
      "DOI": "10.1084/jem.186.11.1819",
      "volume": "186",
      "year": "1997",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072513044800200_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "6466",
      "DOI": "10.4049/jimmunol.171.12.6466",
      "volume": "171",
      "year": "2003",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072513044800200_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "6079",
      "DOI": "10.4049/jimmunol.169.11.6079",
      "volume": "169",
      "year": "2002",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072513044800200_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "1164",
      "DOI": "10.1016/S0198-8859(02)00755-3",
      "volume": "63",
      "year": "2002",
      "journal-title": "Hum. Immunol."
    },
    {
      "key": "2023072513044800200_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "3388",
      "DOI": "10.1002/1521-4141(200111)31:11<3388::AID-IMMU3388>3.0.CO;2-Q",
      "volume": "31",
      "year": "2001",
      "journal-title": "Eur. J. Immunol."
    },
    {
      "key": "2023072513044800200_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "3263",
      "DOI": "10.1182/blood.V99.9.3263",
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      "journal-title": "Blood."
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    {
      "key": "2023072513044800200_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "1947",
      "DOI": "10.4049/jimmunol.161.4.1947",
      "volume": "161",
      "year": "1998",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072513044800200_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "25",
      "DOI": "10.1111/j.1600-065X.1997.tb00956.x",
      "volume": "156",
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      "journal-title": "Immunol. Rev."
    },
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      "key": "2023072513044800200_BIB38",
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      "first-page": "391",
      "DOI": "10.1046/j.1365-3083.1999.00514.x",
      "volume": "49",
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      "first-page": "1862",
      "DOI": "10.4049/jimmunol.167.4.1862",
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      "key": "2023072513044800200_BIB40",
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      "first-page": "823",
      "DOI": "10.1084/jem.20020437",
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