DOI: 10.1084/jem.20031059. CpG-matured Murine Plasmacytoid Dendritic Cells Are Capable of In Vivo Priming of Functional CD8 T Cell Responses to Endogenous but Not Exogenous Antigens

CpG-matured Murine Plasmacytoid Dendritic Cells Are Capable of In Vivo Priming of Functional CD8 T Cell Responses to Endogenous but Not Exogenous Antigens

10.1084/jem.20031059

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Plasmacytoid dendritic cells (PDCs) are a unique leukocyte population capable of secreting high levels of type I interferon (IFN) in response to viruses and bacterial stimuli. In vitro experiments have shown that upon maturation, human and murine PDCs develop into potent immunostimulatory cells; however, their ability to prime an immune response in vivo remains to be addressed. We report that CpG-matured murine PDCs are capable of eliciting in naive mice antigen-specific CTLs against endogenous antigens as well as exogenous peptides, but not against an exogenous antigen. Type I IFN is not required for priming, as injection of CpG-matured PDCs into type I IFN receptor–deficient mice elicits functional CTL responses. Mature PDCs prime CTLs that secrete IFN-γ and protect mice from a tumor challenge. In contrast, immature PDCs are unable to prime antigen-specific CTLs. However, mice injected with immature PDCs are fully responsive to secondary antigenic challenges, suggesting that PDCs have not induced long-lasting tolerance via anergic or regulatory T cells. Our results underline the heterogeneity and plasticity of different antigen-presenting cells, and reveal an important role of mature PDCs in priming CD8 responses to endogenous antigens, in addition to their previously reported ability to modulate antiviral responses via type I IFN.

Bibliography

Salio, M., Palmowski, M. J., Atzberger, A., Hermans, I. F., & Cerundolo, V. (2004). CpG-matured Murine Plasmacytoid Dendritic Cells Are Capable of In Vivo Priming of Functional CD8 T Cell Responses to Endogenous but Not Exogenous Antigens. The Journal of Experimental Medicine, 199(4), 567â579.

Authors 5

Mariolina Salio (first)
Michael J. Palmowski (additional)
Ann Atzberger (additional)
Ian F. Hermans (additional)
Vincenzo Cerundolo (additional)

References 64 Referenced 158

10.1038/32588 / Nature. (1998)
10.1038/nri746 / Nat. Rev. Immunol. (2002)
10.1038/11360 / Nat. Med. (1999)
10.1126/science.284.5421.1835 / Science. (1999)
10.1002/eji.200323676 / Eur. J. Immunol. (2003)
10.1182/blood-2002-10-3063 / Blood. (2003)
10.1084/jem.20011603 / J. Exp. Med. (2002)
10.1038/ni736 / Nat. Immunol. (2001)
10.1182/blood.V98.13.3520 / Blood. (2001)
10.1084/jem.194.8.1171 / J. Exp. Med. (2001)
10.1084/jem.20021091 / J. Exp. Med. (2003)
10.4049/jimmunol.169.12.6711 / J. Immunol. (2002)
10.1182/blood.V100.2.383 / Blood. (2002)
10.1182/blood-2002-03-0974 / Blood. (2003)
10.1084/jem.20021908 / J. Exp. Med. (2003)
10.1046/j.1365-2567.2003.01606.x / Immunology. (2003)
{'key': '2023072512282183200_BIB17'}
10.1084/jem.20020045 / J. Exp. Med. (2002)
10.1126/science.8009221 / Science. (1994)
10.1126/science.274.5284.94 / Science. (1996)
10.1006/viro.1997.8627 / Virology. (1997)
10.4049/jimmunol.154.9.4414 / J. Immunol. (1995)
10.1002/(SICI)1521-4141(199907)29:07<2213::AID-IMMU2213>3.0.CO;2-8 / Eur. J. Immunol. (1999)
10.4049/jimmunol.168.9.4391 / J. Immunol. (2002)
{'key': '2023072512282183200_BIB25'}
10.4049/jimmunol.161.5.2187 / J. Immunol. (1998)
10.1084/jem.189.5.821 / J. Exp. Med. (1999)
10.4049/jimmunol.167.7.3756 / J. Immunol. (2001)
10.1038/ng1296-474 / Nat. Genet. (1996)
10.1084/jem.143.5.1283 / J. Exp. Med. (1976)
10.1038/18038 / Nature. (1999)
10.4049/jimmunol.169.10.5522 / J. Immunol. (2002)
10.1002/1521-4141(200206)32:6<1688::AID-IMMU1688>3.0.CO;2-9 / Eur. J. Immunol. (2002)
10.1084/jem.189.3.521 / J. Exp. Med. (1999)
10.1111/j.1600-065X.1999.tb01327.x / Immunol. Rev. (1999)
10.1084/jem.20021542 / J. Exp. Med. (2003)
10.1084/jem.20021522 / J. Exp. Med. (2003)
10.1038/333742a0 / Nature. (1988)
10.1006/jsre.1993.1069 / J. Surg. Res. (1993)
10.1126/science.1900951 / Science. (1991)
10.1093/intimm/9.10.1423 / Int. Immunol. (1997)
10.1126/science.283.5408.1745 / Science. (1999)
10.1084/jem.193.2.233 / J. Exp. Med. (2001)
10.1084/jem.192.9.1213 / J. Exp. Med. (2000)
10.1084/jem.20021598 / J. Exp. Med. (2002)
10.1182/blood-2002-10-3229 / Blood. (2003)
10.1084/jem.20011672 / J. Exp. Med. (2002)
{'key': '2023072512282183200_BIB48', 'first-page': '69', 'volume': '4', 'year': '2003', 'journal-title': 'Nat. Immunol.'} / Nat. Immunol. (2003)
10.1038/ni873 / Nat. Immunol. (2003)
10.1182/blood-2002-04-1088 / Blood. (2003)
10.1084/jem.20021263 / J. Exp. Med. (2002)
10.4049/jimmunol.170.3.1131 / J. Immunol. (2003)
10.4049/jimmunol.170.6.3059 / J. Immunol. (2003)
10.1073/pnas.1934678100 / Proc. Natl. Acad. Sci. USA. (2003)
10.1093/intimm/dxf089 / Int. Immunol. (2002)
10.1084/jem.20030645 / J. Exp. Med. (2003)
10.1084/jem.185.6.1101 / J. Exp. Med. (1997)
10.1182/blood.V93.7.2302 / Blood. (1999)
10.1084/jem.193.8.881 / J. Exp. Med. (2001)
10.1084/jem.192.12.1685 / J. Exp. Med. (2000)
10.1084/jem.20020161 / J. Exp. Med. (2002)
10.4049/jimmunol.166.9.5327 / J. Immunol. (2001)
10.1084/jem.189.3.587 / J. Exp. Med. (1999)
10.1038/ni978 / Nat. Immunol. (2003)

Dates

Type	When
Created	21 years, 6 months ago (Feb. 17, 2004, 6:23 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 8:39 a.m.)
Indexed	1 month ago (July 24, 2025, 7:57 a.m.)
Issued	21 years, 6 months ago (Feb. 16, 2004)
Published	21 years, 6 months ago (Feb. 16, 2004)
Published Online	21 years, 6 months ago (Feb. 17, 2004)
Published Print	21 years, 6 months ago (Feb. 16, 2004)

Funders 0

None

BibTeX

@article{Salio_2004, title={CpG-matured Murine Plasmacytoid Dendritic Cells Are Capable of In Vivo Priming of Functional CD8 T Cell Responses to Endogenous but Not Exogenous Antigens}, volume={199}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20031059}, DOI={10.1084/jem.20031059}, number={4}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Salio, Mariolina and Palmowski, Michael J. and Atzberger, Ann and Hermans, Ian F. and Cerundolo, Vincenzo}, year={2004}, month=feb, pages={567–579} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        24
      ]
    ],
    "date-time": "2025-07-24T11:57:19Z",
    "timestamp": 1753358239165
  },
  "reference-count": 64,
  "publisher": "Rockefeller University Press",
  "issue": "4",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        2,
        16
      ]
    ]
  },
  "abstract": "<jats:p>Plasmacytoid dendritic cells (PDCs) are a unique leukocyte population capable of secreting high levels of type I interferon (IFN) in response to viruses and bacterial stimuli. In vitro experiments have shown that upon maturation, human and murine PDCs develop into potent immunostimulatory cells; however, their ability to prime an immune response in vivo remains to be addressed. We report that CpG-matured murine PDCs are capable of eliciting in naive mice antigen-specific CTLs against endogenous antigens as well as exogenous peptides, but not against an exogenous antigen. Type I IFN is not required for priming, as injection of CpG-matured PDCs into type I IFN receptor\u2013deficient mice elicits functional CTL responses. Mature PDCs prime CTLs that secrete IFN-\u03b3 and protect mice from a tumor challenge. In contrast, immature PDCs are unable to prime antigen-specific CTLs. However, mice injected with immature PDCs are fully responsive to secondary antigenic challenges, suggesting that PDCs have not induced long-lasting tolerance via anergic or regulatory T cells. Our results underline the heterogeneity and plasticity of different antigen-presenting cells, and reveal an important role of mature PDCs in priming CD8 responses to endogenous antigens, in addition to their previously reported ability to modulate antiviral responses via type I IFN.</jats:p>",
  "DOI": "10.1084/jem.20031059",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        2,
        17
      ]
    ],
    "date-time": "2004-02-17T23:23:47Z",
    "timestamp": 1077060227000
  },
  "page": "567-579",
  "update-policy": "http://dx.doi.org/10.1084/jem.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 158,
  "title": "CpG-matured Murine Plasmacytoid Dendritic Cells Are Capable of In Vivo Priming of Functional CD8 T Cell Responses to Endogenous but Not Exogenous Antigens",
  "prefix": "10.1084",
  "volume": "199",
  "author": [
    {
      "given": "Mariolina",
      "family": "Salio",
      "sequence": "first",
      "affiliation": [
        {
          "name": "1Cancer Research Tumor Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DS Oxford, UK"
        }
      ]
    },
    {
      "given": "Michael J.",
      "family": "Palmowski",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Cancer Research Tumor Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DS Oxford, UK"
        }
      ]
    },
    {
      "given": "Ann",
      "family": "Atzberger",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "2Medical Research Council Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DS Oxford, UK"
        }
      ]
    },
    {
      "given": "Ian F.",
      "family": "Hermans",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Cancer Research Tumor Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DS Oxford, UK"
        }
      ]
    },
    {
      "given": "Vincenzo",
      "family": "Cerundolo",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Cancer Research Tumor Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DS Oxford, UK"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        2004,
        2,
        17
      ]
    ]
  },
  "reference": [
    {
      "key": "2023072512282183200_BIB1",
      "doi-asserted-by": "crossref",
      "first-page": "245",
      "DOI": "10.1038/32588",
      "volume": "392",
      "year": "1998",
      "journal-title": "Nature."
    },
    {
      "key": "2023072512282183200_BIB2",
      "doi-asserted-by": "crossref",
      "first-page": "151",
      "DOI": "10.1038/nri746",
      "volume": "2",
      "year": "2002",
      "journal-title": "Nat. Rev. Immunol."
    },
    {
      "key": "2023072512282183200_BIB3",
      "doi-asserted-by": "crossref",
      "first-page": "919",
      "DOI": "10.1038/11360",
      "volume": "5",
      "year": "1999",
      "journal-title": "Nat. Med."
    },
    {
      "key": "2023072512282183200_BIB4",
      "doi-asserted-by": "crossref",
      "first-page": "1835",
      "DOI": "10.1126/science.284.5421.1835",
      "volume": "284",
      "year": "1999",
      "journal-title": "Science."
    },
    {
      "key": "2023072512282183200_BIB5",
      "doi-asserted-by": "crossref",
      "first-page": "1052",
      "DOI": "10.1002/eji.200323676",
      "volume": "33",
      "year": "2003",
      "journal-title": "Eur. J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "3520",
      "DOI": "10.1182/blood-2002-10-3063",
      "volume": "101",
      "year": "2003",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "695",
      "DOI": "10.1084/jem.20011603",
      "volume": "195",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "1144",
      "DOI": "10.1038/ni736",
      "volume": "2",
      "year": "2001",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "2023072512282183200_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "3520",
      "DOI": "10.1182/blood.V98.13.3520",
      "volume": "98",
      "year": "2001",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB10",
      "doi-asserted-by": "crossref",
      "first-page": "1171",
      "DOI": "10.1084/jem.194.8.1171",
      "volume": "194",
      "year": "2001",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB11",
      "doi-asserted-by": "crossref",
      "first-page": "899",
      "DOI": "10.1084/jem.20021091",
      "volume": "197",
      "year": "2003",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB12",
      "doi-asserted-by": "crossref",
      "first-page": "6711",
      "DOI": "10.4049/jimmunol.169.12.6711",
      "volume": "169",
      "year": "2002",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB13",
      "doi-asserted-by": "crossref",
      "first-page": "383",
      "DOI": "10.1182/blood.V100.2.383",
      "volume": "100",
      "year": "2002",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "1453",
      "DOI": "10.1182/blood-2002-03-0974",
      "volume": "101",
      "year": "2003",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB15",
      "doi-asserted-by": "crossref",
      "first-page": "101",
      "DOI": "10.1084/jem.20021908",
      "volume": "197",
      "year": "2003",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "481",
      "DOI": "10.1046/j.1365-2567.2003.01606.x",
      "volume": "108",
      "year": "2003",
      "journal-title": "Immunology."
    },
    {
      "key": "2023072512282183200_BIB17"
    },
    {
      "key": "2023072512282183200_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "953",
      "DOI": "10.1084/jem.20020045",
      "volume": "195",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "1918",
      "DOI": "10.1126/science.8009221",
      "volume": "264",
      "year": "1994",
      "journal-title": "Science."
    },
    {
      "key": "2023072512282183200_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "94",
      "DOI": "10.1126/science.274.5284.94",
      "volume": "274",
      "year": "1996",
      "journal-title": "Science."
    },
    {
      "key": "2023072512282183200_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "62",
      "DOI": "10.1006/viro.1997.8627",
      "volume": "234",
      "year": "1997",
      "journal-title": "Virology."
    },
    {
      "key": "2023072512282183200_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "4414",
      "DOI": "10.4049/jimmunol.154.9.4414",
      "volume": "154",
      "year": "1995",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "2213",
      "DOI": "10.1002/(SICI)1521-4141(199907)29:07<2213::AID-IMMU2213>3.0.CO;2-8",
      "volume": "29",
      "year": "1999",
      "journal-title": "Eur. J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "4391",
      "DOI": "10.4049/jimmunol.168.9.4391",
      "volume": "168",
      "year": "2002",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB25"
    },
    {
      "key": "2023072512282183200_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "2187",
      "DOI": "10.4049/jimmunol.161.5.2187",
      "volume": "161",
      "year": "1998",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "821",
      "DOI": "10.1084/jem.189.5.821",
      "volume": "189",
      "year": "1999",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "3756",
      "DOI": "10.4049/jimmunol.167.7.3756",
      "volume": "167",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "474",
      "DOI": "10.1038/ng1296-474",
      "volume": "14",
      "year": "1996",
      "journal-title": "Nat. Genet."
    },
    {
      "key": "2023072512282183200_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "1283",
      "DOI": "10.1084/jem.143.5.1283",
      "volume": "143",
      "year": "1976",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1038/18038",
      "volume": "398",
      "year": "1999",
      "journal-title": "Nature."
    },
    {
      "key": "2023072512282183200_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "5522",
      "DOI": "10.4049/jimmunol.169.10.5522",
      "volume": "169",
      "year": "2002",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "1688",
      "DOI": "10.1002/1521-4141(200206)32:6<1688::AID-IMMU1688>3.0.CO;2-9",
      "volume": "32",
      "year": "2002",
      "journal-title": "Eur. J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "521",
      "DOI": "10.1084/jem.189.3.521",
      "volume": "189",
      "year": "1999",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "39",
      "DOI": "10.1111/j.1600-065X.1999.tb01327.x",
      "volume": "170",
      "year": "1999",
      "journal-title": "Immunol. Rev."
    },
    {
      "key": "2023072512282183200_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "111",
      "DOI": "10.1084/jem.20021542",
      "volume": "198",
      "year": "2003",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "885",
      "DOI": "10.1084/jem.20021522",
      "volume": "197",
      "year": "2003",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "742",
      "DOI": "10.1038/333742a0",
      "volume": "333",
      "year": "1988",
      "journal-title": "Nature."
    },
    {
      "key": "2023072512282183200_BIB39",
      "doi-asserted-by": "crossref",
      "first-page": "437",
      "DOI": "10.1006/jsre.1993.1069",
      "volume": "54",
      "year": "1993",
      "journal-title": "J. Surg. Res."
    },
    {
      "key": "2023072512282183200_BIB40",
      "doi-asserted-by": "crossref",
      "first-page": "1225",
      "DOI": "10.1126/science.1900951",
      "volume": "251",
      "year": "1991",
      "journal-title": "Science."
    },
    {
      "key": "2023072512282183200_BIB41",
      "doi-asserted-by": "crossref",
      "first-page": "1423",
      "DOI": "10.1093/intimm/9.10.1423",
      "volume": "9",
      "year": "1997",
      "journal-title": "Int. Immunol."
    },
    {
      "key": "2023072512282183200_BIB42",
      "doi-asserted-by": "crossref",
      "first-page": "1745",
      "DOI": "10.1126/science.283.5408.1745",
      "volume": "283",
      "year": "1999",
      "journal-title": "Science."
    },
    {
      "key": "2023072512282183200_BIB43",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1084/jem.193.2.233",
      "volume": "193",
      "year": "2001",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB44",
      "doi-asserted-by": "crossref",
      "first-page": "1213",
      "DOI": "10.1084/jem.192.9.1213",
      "volume": "192",
      "year": "2000",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB45",
      "doi-asserted-by": "crossref",
      "first-page": "1627",
      "DOI": "10.1084/jem.20021598",
      "volume": "196",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB46",
      "doi-asserted-by": "crossref",
      "first-page": "4862",
      "DOI": "10.1182/blood-2002-10-3229",
      "volume": "101",
      "year": "2003",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB47",
      "doi-asserted-by": "crossref",
      "first-page": "517",
      "DOI": "10.1084/jem.20011672",
      "volume": "195",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB48",
      "first-page": "69",
      "volume": "4",
      "year": "2003",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "2023072512282183200_BIB49",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1038/ni873",
      "volume": "4",
      "year": "2003",
      "journal-title": "Nat. Immunol."
    },
    {
      "key": "2023072512282183200_BIB50",
      "doi-asserted-by": "crossref",
      "first-page": "305",
      "DOI": "10.1182/blood-2002-04-1088",
      "volume": "101",
      "year": "2003",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB51",
      "doi-asserted-by": "crossref",
      "first-page": "1415",
      "DOI": "10.1084/jem.20021263",
      "volume": "196",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB52",
      "doi-asserted-by": "crossref",
      "first-page": "1131",
      "DOI": "10.4049/jimmunol.170.3.1131",
      "volume": "170",
      "year": "2003",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB53",
      "doi-asserted-by": "crossref",
      "first-page": "3059",
      "DOI": "10.4049/jimmunol.170.6.3059",
      "volume": "170",
      "year": "2003",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB54",
      "doi-asserted-by": "crossref",
      "first-page": "10872",
      "DOI": "10.1073/pnas.1934678100",
      "volume": "100",
      "year": "2003",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072512282183200_BIB55",
      "doi-asserted-by": "crossref",
      "first-page": "1225",
      "DOI": "10.1093/intimm/dxf089",
      "volume": "14",
      "year": "2002",
      "journal-title": "Int. Immunol."
    },
    {
      "key": "2023072512282183200_BIB56",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1084/jem.20030645",
      "volume": "198",
      "year": "2003",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB57",
      "doi-asserted-by": "crossref",
      "first-page": "1101",
      "DOI": "10.1084/jem.185.6.1101",
      "volume": "185",
      "year": "1997",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB58",
      "doi-asserted-by": "crossref",
      "first-page": "2302",
      "DOI": "10.1182/blood.V93.7.2302",
      "volume": "93",
      "year": "1999",
      "journal-title": "Blood."
    },
    {
      "key": "2023072512282183200_BIB59",
      "doi-asserted-by": "crossref",
      "first-page": "881",
      "DOI": "10.1084/jem.193.8.881",
      "volume": "193",
      "year": "2001",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB60",
      "doi-asserted-by": "crossref",
      "first-page": "1685",
      "DOI": "10.1084/jem.192.12.1685",
      "volume": "192",
      "year": "2000",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB61",
      "doi-asserted-by": "crossref",
      "first-page": "1289",
      "DOI": "10.1084/jem.20020161",
      "volume": "195",
      "year": "2002",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB62",
      "doi-asserted-by": "crossref",
      "first-page": "5327",
      "DOI": "10.4049/jimmunol.166.9.5327",
      "volume": "166",
      "year": "2001",
      "journal-title": "J. Immunol."
    },
    {
      "key": "2023072512282183200_BIB63",
      "doi-asserted-by": "crossref",
      "first-page": "587",
      "DOI": "10.1084/jem.189.3.587",
      "volume": "189",
      "year": "1999",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "2023072512282183200_BIB64",
      "doi-asserted-by": "crossref",
      "first-page": "1009",
      "DOI": "10.1038/ni978",
      "volume": "4",
      "year": "2003",
      "journal-title": "Nat. Immunol."
    }
  ],
  "container-title": "The Journal of Experimental Medicine",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jem/article-pdf/199/4/567/1713530/jem1994567.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jem/article-pdf/199/4/567/1713530/jem1994567.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        25
      ]
    ],
    "date-time": "2023-07-25T12:39:19Z",
    "timestamp": 1690288759000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jem/article/199/4/567/40077/CpG-matured-Murine-Plasmacytoid-Dendritic-Cells"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        2,
        16
      ]
    ]
  },
  "references-count": 64,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2004,
          2,
          16
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1084/jem.20031059",
  "relation": {},
  "ISSN": [
    "1540-9538",
    "0022-1007"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2004,
        2,
        16
      ]
    ]
  }
}