DOI: 10.1084/jem.20020470. Dectin-1 Is A Major β-Glucan Receptor On Macrophages

Dectin-1 Is A Major β-Glucan Receptor On Macrophages

10.1084/jem.20020470

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Zymosan is a β-glucan– and mannan-rich particle that is widely used as a cellular activator for examining the numerous responses effected by phagocytes. The macrophage mannose receptor (MR) and complement receptor 3 (CR3) have historically been considered the major macrophage lectins involved in the nonopsonic recognition of these yeast-derived particles. Using specific carbohydrate inhibitors, we show that a β-glucan receptor, but not the MR, is a predominant receptor involved in this process. Furthermore, nonopsonic zymosan binding was unaffected by genetic CD11b deficiency or a blocking monoclonal antibody (mAb) against CR3, demonstrating that CR3 was not the β-glucan receptor mediating this activity. To address the role of the recently described β-glucan receptor, Dectin-1, we generated a novel anti–Dectin-1 mAb, 2A11. Using this mAb, we show here that Dectin-1 was almost exclusively responsible for the β-glucan–dependent, nonopsonic recognition of zymosan by primary macro-phages. These findings define Dectin-1 as the leukocyte β-glucan receptor, first described over 50 years ago, and resolves the long-standing controversy regarding the identity of this important molecule. Furthermore, these results identify Dectin-1 as a new target for examining the immunomodulatory properties of β-glucans for therapeutic drug design.

Bibliography

Brown, G. D., Taylor, P. R., Reid, D. M., Willment, J. A., Williams, D. L., Martinez-Pomares, L., Wong, S. Y. C., & Gordon, S. (2002). Dectin-1 Is A Major Î²-Glucan Receptor On Macrophages. The Journal of Experimental Medicine, 196(3), 407â412.

Authors 8

Gordon D. Brown (first)
Philip R. Taylor (additional)
Delyth M. Reid (additional)
Janet A. Willment (additional)
David L. Williams (additional)
Luisa Martinez-Pomares (additional)
Simon Y.C. Wong (additional)
Siamon Gordon (additional)

References 30 Referenced 852

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Dates

Type	When
Created	22 years, 10 months ago (Sept. 30, 2002, 1:03 p.m.)
Deposited	2 years ago (July 25, 2023, 7:44 a.m.)
Indexed	1 week, 3 days ago (Aug. 12, 2025, 5:31 p.m.)
Issued	23 years ago (July 29, 2002)
Published	23 years ago (July 29, 2002)
Published Online	23 years ago (July 29, 2002)
Published Print	23 years ago (Aug. 5, 2002)

Funders 0

None

BibTeX

@article{Brown_2002, title={Dectin-1 Is A Major β-Glucan Receptor On Macrophages}, volume={196}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20020470}, DOI={10.1084/jem.20020470}, number={3}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Brown, Gordon D. and Taylor, Philip R. and Reid, Delyth M. and Willment, Janet A. and Williams, David L. and Martinez-Pomares, Luisa and Wong, Simon Y.C. and Gordon, Siamon}, year={2002}, month=jul, pages={407–412} }

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