DOI: 10.1084/jem.20020161. The CD8+ Dendritic Cell Subset Selectively Endocytoses Dying Cells in Culture and In Vivo

The CD8+ Dendritic Cell Subset Selectively Endocytoses Dying Cells in Culture and In Vivo

10.1084/jem.20020161

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Dendritic cells (DCs) are able in tissue culture to phagocytose and present antigens derived from infected, malignant, and allogeneic cells. Here we show directly that DCs in situ take up these types of cells after fluorescent labeling with carboxyfluorescein succinimidyl ester (CFSE) and injection into mice. The injected cells include syngeneic splenocytes and tumor cell lines, induced to undergo apoptosis ex vivo by exposure to osmotic shock, and allogeneic B cells killed by NK cells in situ. The CFSE-labeled cells in each case are actively endocytosed by DCs in vivo, but only the CD8+ subset. After uptake, all of the phagocytic CD8+ DCs can form major histocompatibility complex class II–peptide complexes, as detected with a monoclonal antibody specific for these complexes. The CD8+ DCs also selectively present cell-associated antigens to both CD4+ and CD8+ T cells. Similar events take place with cultured DCs; CD8+ DCs again selectively take up and present dying cells. In contrast, both CD8+ and CD8− DCs phagocytose latex particles in culture, and both DC subsets present soluble ovalbumin captured in vivo. Therefore CD8+ DCs are specialized to capture dying cells, and this helps to explain their selective ability to cross present cellular antigens to both CD4+ and CD8+ T cells.

Bibliography

Iyoda, T., Shimoyama, S., Liu, K., Omatsu, Y., Akiyama, Y., Maeda, Y., Takahara, K., Steinman, R. M., & Inaba, K. (2002). The CD8+ Dendritic Cell Subset Selectively Endocytoses Dying Cells in Culture and In Vivo. The Journal of Experimental Medicine, 195(10), 1289â1302.

Authors 9

Tomonori Iyoda (first)
Susumu Shimoyama (additional)
Kang Liu (additional)
Yoshiki Omatsu (additional)
Yuji Akiyama (additional)
Yasuhiro Maeda (additional)
Kazuhiko Takahara (additional)
Ralph M. Steinman (additional)
Kayo Inaba (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 7:20 a.m.)
Indexed	3 days, 7 hours ago (Aug. 30, 2025, 12:59 p.m.)
Issued	23 years, 3 months ago (May 20, 2002)
Published	23 years, 3 months ago (May 20, 2002)
Published Online	23 years, 3 months ago (May 20, 2002)
Published Print	23 years, 3 months ago (May 20, 2002)

Funders 0

None

BibTeX

@article{Iyoda_2002, title={The CD8+ Dendritic Cell Subset Selectively Endocytoses Dying Cells in Culture and In Vivo}, volume={195}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20020161}, DOI={10.1084/jem.20020161}, number={10}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Iyoda, Tomonori and Shimoyama, Susumu and Liu, Kang and Omatsu, Yoshiki and Akiyama, Yuji and Maeda, Yasuhiro and Takahara, Kazuhiko and Steinman, Ralph M. and Inaba, Kayo}, year={2002}, month=may, pages={1289–1302} }

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