DOI: 10.1084/jem.20011500. Lipid Raft Microdomains

10.1084/jem.20011500

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.

Bibliography

Bavari, S., Bosio, C. M., Wiegand, E., Ruthel, G., Will, A. B., Geisbert, T. W., Hevey, M., Schmaljohn, C., Schmaljohn, A., & Aman, M. J. (2002). Lipid Raft Microdomains. The Journal of Experimental Medicine, 195(5), 593â602.

Authors 10

Sina Bavari (first)
Catharine M. Bosio (additional)
Elizabeth Wiegand (additional)
Gordon Ruthel (additional)
Amy B. Will (additional)
Thomas W. Geisbert (additional)
Michael Hevey (additional)
Connie Schmaljohn (additional)
Alan Schmaljohn (additional)
M. Javad Aman (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 7:33 a.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 6:44 a.m.)
Issued	23 years, 5 months ago (March 4, 2002)
Published	23 years, 5 months ago (March 4, 2002)
Published Online	23 years, 5 months ago (March 4, 2002)
Published Print	23 years, 5 months ago (March 4, 2002)

Funders 0

None

BibTeX

@article{Bavari_2002, title={Lipid Raft Microdomains}, volume={195}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20011500}, DOI={10.1084/jem.20011500}, number={5}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Bavari, Sina and Bosio, Catharine M. and Wiegand, Elizabeth and Ruthel, Gordon and Will, Amy B. and Geisbert, Thomas W. and Hevey, Michael and Schmaljohn, Connie and Schmaljohn, Alan and Aman, M. Javad}, year={2002}, month=mar, pages={593–602} }

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