DOI: 10.1084/jem.20010898. Major Histocompatibility Complex Class II Presentation of Cell-associated Antigen Is Mediated by CD8α+ Dendritic Cells In Vivo

Major Histocompatibility Complex Class II Presentation of Cell-associated Antigen Is Mediated by CD8α+ Dendritic Cells In Vivo

10.1084/jem.20010898

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Antigen-specific B cells express major histocompatibility complex class II and can present antigen directly to T cells. Adoptive transfer experiments using transgenic B and T cells demonstrated that antigen-specific B cells can also efficiently transfer antigen to another cell for presentation to T cells in vivo. To identify the antigen-presenting cell that receives antigens from B cells, a strategy was developed to follow the traffic of B cell–derived proteins in vivo. B cells were labeled with the fluorescent dye CFSE and loaded with antigen, before adoptive transfer into recipient mice. Populations of splenocytes from the recipient mice were later assayed for the presence of fluorescent proteins and for the ability to activate T cells. A small number of CD8α+CD4−CD11blo dendritic cells (DCs) contain proteins transferred from B cells and these DCs effectively present antigens derived from the B cells to T cells. The results suggest that CD8α+ DCs sample the cells and membranes in their environment for presentation to T cells circulating through the T cell zone. This function of CD8α+ DCs may be relevant to the priming of an immune response or the induction of T cell tolerance.

Bibliography

Valdez, Y., Mah, W., Winslow, M. M., Xu, L., Ling, P., & Townsend, S. E. (2002). Major Histocompatibility Complex Class II Presentation of Cell-associated Antigen Is Mediated by CD8Î±+ Dendritic Cells In Vivo. The Journal of Experimental Medicine, 195(6), 683â694.

Authors 6

Yanet Valdez (first)
Weiling Mah (additional)
Monte M. Winslow (additional)
Lixin Xu (additional)
Peter Ling (additional)
Sarah E. Townsend (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 7:33 a.m.)
Indexed	3 weeks ago (Aug. 12, 2025, 5:57 p.m.)
Issued	23 years, 5 months ago (March 11, 2002)
Published	23 years, 5 months ago (March 11, 2002)
Published Online	23 years, 5 months ago (March 11, 2002)
Published Print	23 years, 5 months ago (March 18, 2002)

Funders 0

None

BibTeX

@article{Valdez_2002, title={Major Histocompatibility Complex Class II Presentation of Cell-associated Antigen Is Mediated by CD8α+ Dendritic Cells In Vivo}, volume={195}, ISSN={0022-1007}, url={http://dx.doi.org/10.1084/jem.20010898}, DOI={10.1084/jem.20010898}, number={6}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Valdez, Yanet and Mah, Weiling and Winslow, Monte M. and Xu, Lixin and Ling, Peter and Townsend, Sarah E.}, year={2002}, month=mar, pages={683–694} }

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