DOI: 10.1084/jem.192.6.823. Interleukin (Il)-4 Is a Major Regulatory Cytokine Governing Bioactive IL-12 Production by Mouse and Human Dendritic Cells

Interleukin (Il)-4 Is a Major Regulatory Cytokine Governing Bioactive IL-12 Production by Mouse and Human Dendritic Cells

10.1084/jem.192.6.823

Crossref journal-article

Rockefeller University Press

The Journal of Experimental Medicine (291)

Abstract

Interleukin (IL)-12 may be secreted as a bioactive T helper type 1 (Th1) cell–inducing heterodimer, as a monomer, or as an antagonistic homodimer. We analyzed the IL-12 produced by mouse splenic dendritic cells (DCs), human thymic DCs, and cultured human monocyte-derived DCs. IL-12 production required both a microbial or T cell–derived stimulus and an appropriate cytokine milieu. The different IL-12 forms were differentially regulated by the cytokines present rather than the stimulus used. IL-4 alone or together with granulocyte/macrophage colony-stimulating factor or interferon γ effectively enhanced the production of the bioactive heterodimer and selectively reduced the antagonistic homodimer of IL-12. Therefore, IL-4, the major Th2-driving cytokine, provides a negative feedback causing DCs to produce the major Th1-inducing cytokine, bioactive IL-12.

Bibliography

Hochrein, H., OâKeeffe, M., Luft, T., Vandenabeele, S., Grumont, R. J., Maraskovsky, E., & Shortman, K. (2000). Interleukin (Il)-4 Is a Major Regulatory Cytokine Governing Bioactive IL-12 Production by Mouse and Human Dendritic Cells. The Journal of Experimental Medicine, 192(6), 823â834.

Authors 7

Hubertus Hochrein (first)
Meredith O'Keeffe (additional)
Thomas Luft (additional)
Stéphane Vandenabeele (additional)
Raelene J. Grumont (additional)
Eugene Maraskovsky (additional)
Ken Shortman (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:48 p.m.)
Deposited	2 years, 1 month ago (July 25, 2023, 6:41 a.m.)
Indexed	4 months, 3 weeks ago (April 9, 2025, 2:02 a.m.)
Issued	24 years, 11 months ago (Sept. 11, 2000)
Published	24 years, 11 months ago (Sept. 11, 2000)
Published Online	24 years, 11 months ago (Sept. 11, 2000)
Published Print	24 years, 11 months ago (Sept. 18, 2000)

Funders 0

None

BibTeX

@article{Hochrein_2000, title={Interleukin (Il)-4 Is a Major Regulatory Cytokine Governing Bioactive IL-12 Production by Mouse and Human Dendritic Cells}, volume={192}, ISSN={1540-9538}, url={http://dx.doi.org/10.1084/jem.192.6.823}, DOI={10.1084/jem.192.6.823}, number={6}, journal={The Journal of Experimental Medicine}, publisher={Rockefeller University Press}, author={Hochrein, Hubertus and O’Keeffe, Meredith and Luft, Thomas and Vandenabeele, Stéphane and Grumont, Raelene J. and Maraskovsky, Eugene and Shortman, Ken}, year={2000}, month=sep, pages={823–834} }

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